Molecular mechanism and control of a fungal exocytosis pathway in the plant pathogens Ustilago maydis and Mycosphaerella graminicola

植物病原体玉米黑粉菌和禾本科球腔菌真菌胞吐途径的分子机制和控制

• 批准号: BB/I020667/1
• 负责人: Gero Steinberg
• 金额: $ 45.3万
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI020667%2F1
• 关键词: Molecular; mechanism; control; fungal; exocytosis

Pathogenic fungi are a major threat to human food security. While they are controlled by active pest management, fungi rapidly adjust and develop resistances. Thus, research on fungal specific and pathogenicity-relevant cellular processes is required to identify new targets for fungicide development. Fungi invade the host tissue by expanding at the end of their elongated cells. This process is named 'tip growth' and it involves the polar release of enzymes that participate in the formation of the fungal cell wall. We have recently reported that a particular fungal-specific myosin-chitin synthase-like protein (Mcs1) has a key role in tip growth in the corn smut fungus Ustilago maydis. This protein is also found in many other pathogenic fungi, where it also essential for plant infection. In this project we will join up with an industrial partner, Syngenta Ltd., to identify regulators that control this important Mcs1 protein. We will elucidate the role of a so far unknown Mcs1 protein in the fungus Mycosphaerella graminicola, the causal agent of Septoria tritici blotch on wheat, which is the most devastating wheat pathogen in the UK and Europe. In addition, we will use our existing knowledge and molecular tools in U. maydis to identify new factors that control the function of Mcs1. When considered together the project promises to provide fundamental new insight into a fungal specific pathway, essential for plant infection. This will provide knowledge that will help to develop new fungicides and therefore is of high value to the agricultural biotechnology industry in the UK.

病原真菌是人类粮食安全的主要威胁。当它们受到积极的害虫管理控制时，真菌迅速调整并产生抗性。因此，需要对真菌特异性和致病性相关的细胞过程进行研究，以确定杀菌剂开发的新目标。真菌通过在其细长细胞的末端扩张来侵入宿主组织。这个过程被称为“尖端生长”，它涉及参与真菌细胞壁形成的酶的极性释放。我们最近报道了一种特殊的真菌特异性肌球蛋白几丁质酶样蛋白（Mcs1）在玉米黑粉菌的茎尖生长中起着关键作用。这种蛋白质也存在于许多其他病原真菌中，在那里它也是植物感染所必需的。在这个项目中，我们将与工业合作伙伴先正达有限公司合作，来鉴定控制这种重要的Mcs1蛋白的调节因子。我们将阐明迄今为止未知的Mcs1蛋白在真菌禾生球腔菌中的作用，禾生球腔菌是小麦上壳针孢菌斑点病的病原体，小麦上壳针孢菌斑点病是英国和欧洲最具破坏性的小麦病原体。此外，我们将利用我们现有的知识和分子工具在美国。maydis来鉴定控制Mcs1功能的新因子。当一起考虑时，该项目有望为植物感染所必需的真菌特异性途径提供基本的新见解。这将提供有助于开发新杀菌剂的知识，因此对英国农业生物技术产业具有很高的价值。

项目成果

Cell biology of Zymoseptoria tritici: Pathogen cell organization and wheat infection.

• DOI: 10.1016/j.fgb.2015.04.002
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Steinberg G

Fluorescent markers for the Spitzenkörper and exocytosis in Zymoseptoria tritici.

• DOI: 10.1016/j.fgb.2015.04.014
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Guo M;Kilaru S;Schuster M;Latz M;Steinberg G
• 通讯作者: Steinberg G

A codon-optimized green fluorescent protein for live cell imaging in Zymoseptoria tritici.

• DOI: 10.1016/j.fgb.2015.03.022
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Kilaru S;Schuster M;Studholme D;Soanes D;Lin C;Talbot NJ;Steinberg G
• 通讯作者: Steinberg G

Conditional promoters for analysis of essential genes in Zymoseptoria tritici.

• DOI: 10.1016/j.fgb.2015.03.024
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Kilaru S;Ma W;Schuster M;Courbot M;Steinberg G
• 通讯作者: Steinberg G

Libraries for two-hybrid screening of yeast and hyphal growth forms in Zymoseptoria tritici.

• DOI: 10.1016/j.fgb.2015.03.023
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Ma W;Kilaru S;Collins C;Courbot M;Steinberg G
• 通讯作者: Steinberg G