Confocal Laser Scanning Microscopy to Investigate Cellular Dynamics in Host-Pathogen Interactions

共焦激光扫描显微镜研究宿主与病原体相互作用的细胞动力学

• 批准号: BB/L014866/1
• 负责人: Gero Steinberg
• 金额: $ 46.47万
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL014866%2F1
• 关键词: Confocal; Laser; Scanning; Microscopy; Investigate

Two of the major global challenges for the next century will be how to ensure global food security and how to contend with emerging diseases which threaten ecosystem health. It emerges that fungi are a major threat to mankind, as no other group of pathogens are more deadly to crop plants than fungi. Fungal disease accounts for persistent crop losses of 10-12% each year and substantially higher losses in epidemic years. Consequently, fungal diseases decimate our harvests and cause widespread malnutrition and starvation. The burden of disease is increasing as global climate change hastens the geographic spread and the variety of plants infected. The University of Exeter has established itself as a centre of fungal-related plant disease research excellence. Our quest is to better understand the intimacy of the relationship between rice, maize and wheat and their respective fungal pathogens, as these crops occupy some 40% of our global crop-land. We will provide technical knowledge from our work on the rice blast fungus Magnaporthe oryzae, the wheat pathogen Mycosphaerella graminicola and the maize smut fungus Ustilago maydis to gain a better insight into the dynamic exchanges between fungi and their hosts at the interface where they first find conflict. Exeter University currently provides many technical facilities to support their research and to meet this challenge. However, lacking is high-end equipment that allows fast, sensitive live-cell imaging of the pathogen in infected plant tissue. In recent years, this technology has enabled plant-pathologists in the EU to investigate the communication between host plant and the invading pathogen. In this application we seek support to purchase a modern confocal laser scanning microscopy, which will complement the existing facilities at the Exeter Bioimaging Centre.

下一个世纪的两大全球性挑战将是如何确保全球粮食安全和如何对付威胁生态系统健康的新出现的疾病。真菌是对人类的主要威胁，因为没有其他种类的病原体比真菌对农作物更致命。真菌病害每年造成10-12%的作物损失，在流行年份损失更大。因此，真菌疾病大量破坏我们的收成，并造成广泛的营养不良和饥饿。随着全球气候变化加速了受感染植物的地理传播和多样性，疾病的负担正在增加。埃克塞特大学已成为真菌相关植物病害研究的卓越中心。我们的目标是更好地了解水稻、玉米和小麦与其各自的真菌病原体之间的密切关系，因为这些作物占据了全球约40%的耕地。我们将从我们对稻瘟病菌Magnaporthe graminicola，小麦病原体Mycosphaerella graminicola和玉米黑粉病真菌Ustilago maydis的研究中提供技术知识，以更好地了解真菌与其宿主之间的动态交换，在界面上它们首先发现冲突。埃克塞特大学目前提供了许多技术设施，以支持他们的研究，并迎接这一挑战。然而，缺乏的是高端设备，允许快速，灵敏的活细胞成像的病原体在受感染的植物组织。近年来，这项技术使欧盟的植物病理学家能够研究宿主植物和入侵病原体之间的通讯。在这个应用程序中，我们寻求支持，购买一个现代化的共聚焦激光扫描显微镜，这将补充现有的设施在埃克塞特生物成像中心。

项目成果

Yeast recombination-based cloning as an efficient way of constructing vectors for Zymoseptoria tritici.

• DOI: 10.1016/j.fgb.2015.03.017
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Kilaru S;Steinberg G
• 通讯作者: Steinberg G

Fluorescent markers of the endocytic pathway in Zymoseptoria tritici.

• DOI: 10.1016/j.fgb.2015.03.019
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Kilaru S;Schuster M;Latz M;Guo M;Steinberg G
• 通讯作者: Steinberg G

Asynchronous development of Zymoseptoria tritici infection in wheat.

• DOI: 10.1016/j.fgb.2020.103504
• 发表时间: 2021-01
• 期刊: Fungal genetics and biology : FG & B
• 作者: Fantozzi E;Kilaru S;Gurr SJ;Steinberg G
• 通讯作者: Steinberg G

A codon-optimized green fluorescent protein for live cell imaging in Zymoseptoria tritici.

• DOI: 10.1016/j.fgb.2015.03.022
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Kilaru S;Schuster M;Studholme D;Soanes D;Lin C;Talbot NJ;Steinberg G
• 通讯作者: Steinberg G

A gene locus for targeted ectopic gene integration in Zymoseptoria tritici.

• DOI: 10.1016/j.fgb.2015.03.018
• 发表时间: 2015-06
• 期刊: Fungal genetics and biology : FG & B
• 作者: Kilaru S;Schuster M;Latz M;Das Gupta S;Steinberg N;Fones H;Gurr SJ;Talbot NJ;Steinberg G
• 通讯作者: Steinberg G