HUMAN ANTI-H INFLUENZAE B IDOTYPES

人类抗 H 流感双型

• 批准号: 3138297
• 负责人: Alexander H. Lucas
• 金额: $ 12.28万
• 依托单位: CHILDREN'S HOSPITAL & RES CTR AT OAKLAND
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3138297
• 关键词: Haemophilus influenzae; Haemophilus influenzae vaccines; affinity chromatography; antibacterial antibody; antibody specificity; antiidiotype antibody; antiserum; autoradiography; bacterial cytopathogenic effect; bacterial disease; bacterial meningitis; bacterial somatic antigen; bactericidal immunity; cross immunity; disease /disorder model; enzyme linked immunosorbent assay; epiglottis; gel electrophoresis; gene expression; human population study; human subject; hybridomas; immunity; immunoelectrophoresis; immunoglobulin genes; immunoglobulin idiotypes; immunologic techniques; immunological substance; infant animal; infant human (0-1 year); laboratory mouse; laboratory rat; monoclonal antibody; pneumonia; preschool child (1-5); radiotracer; serum; sugar phosphates

Despite the availability of antibiotics, Hemophilus influenzae b remains a leading cause of morbidity and mortality in children. This study proposes to analyze the idiotypic diversity of human antibodies specific for the major antigen and virulence factor of H. influenzae b, the capsular carbohydrate, polyribosyl-ribitol- phosphate (PRP). Using hybridoma methodology, murine monoclonal antibodies specific from cross-reactive idiotopes of PRP-specific antibodies will be prepared. These anti-idiotopic reagents will be used to determine: the extent to which idiotopes are shared between unrelated humans, whether infants and adults express similar idiotypic repertoires, and whether anti-idiotopic antibodies can prime neonatal rats for protection from H. influenzae b infection. Also, attempts will be made to identify isologous human anti-idiotypic antibodies. These studies will provide insight into the variable region diversity and ontogeny of the human anti-PRP antibody repertoire as well as test the feasibility of using anti-idiotypic antibodies as a protective H. influenzae b vaccine. Identification of human idiotypes and anti- idiotypes is necessary for future studies which seek to determine whether anti-idiotypic antibodies regulate B cell expression in the infant and the adult.

尽管有抗生素可用，但流感型血友病B 仍然是儿童发病和死亡率的主要原因。 这项研究建议分析人类的白痴多样性 对主要抗原和毒力因子的特异性抗体 H.流感B，胶囊碳水化合物，多氧蛋白蛋白蛋白基核酸 磷酸盐（PRP）。 使用杂交瘤方法，鼠 来自交叉反应性白痴的单克隆抗体 将制备PRP特异性抗体。 这些抗二元 试剂将用于确定：白痴的程度 无论是婴儿和成年人，在不相关的人之间共享 表达类似的白痴型曲目，以及是否抗二动 抗体可以为新生大鼠提供促进抗体。 流感B感染。 另外，将尝试识别 人类的抗二动抗体。 这些研究会 提供有关可变区域多样性和个体发育的见解 人类抗PRP抗体库以及测试 将抗二动抗体用作保护性H的可行性。 流感B疫苗。 鉴定人类愚蠢和抗 对于以后的研究，愚蠢是企图确定的必要 抗IDiotypic抗体是否调节B细胞表达 婴儿和成人。