Nature's solution to the iron problem: Mechanisms of iron management in ferritins

铁问题的自然解决方案:铁蛋白中铁的管理机制

基本信息

  • 批准号:
    BB/I021884/1
  • 负责人:
  • 金额:
    $ 43.18万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2012
  • 资助国家:
    英国
  • 起止时间:
    2012 至 无数据
  • 项目状态:
    已结题

项目摘要

Iron is essential for virtually all forms of life, playing central roles in many of the reactions on which life depends. Although this metal is highly abundant in the earth's crust, it is largely 'locked up' in minerals that are highly insoluble and this severely limits its availability to living organisms. The scarcity of iron has led to the evolution in microbes of many ingenious mechanisms to obtain sufficient quantities to sustain growth. These include the secretion and re-absorption of small organic molecules that can bind iron very tightly and therefore hoover up what iron there is. Pathogenic bacteria can effectively steal iron from iron-containing proteins in their hosts, and the success of such mechanisms is a key determinant of whether or not a successful infection is established. Another important aspect of how cells overcome the iron problem, is their ability to store iron. This is achieved through a remarkable family of proteins known as ferritins. These can be thought of as being football-like molecules with a hollow centre, inside which thousands of iron atoms can be stored in the form of an iron mineral - one that would form insoluble precipititates were it not for the solubilising effect of the protein coat. The storage of iron serves two important functions. Firstly, it enables microbes to draw on reserves when iron in the immediate environment becomes particularly low, and secondly, it overcomes the potential toxicity of iron that results from the very properties that make it useful to life: without proper control, iron can lead to the generation of reactive oxygen species that can cause severe cellular damage. In this research we propose to better understand how two particular ferritins from two very different microbes take up iron and orchestrate the deposition of their iron mineral cargo, and how they subsequently release it. One of these is from the organism Escherichia coli - a bacterium that has for decades been a workhorse for understanding cellular processes, but which can also be pathogenic. The other ferritin is one that was very recently identified in phytoplankton. These organisms are responsible for a large proportion of the primary production of organic molecules from carbon dioxide in the oceans. Furthermore, they also form spectacular blooms in areas of temporary nutrient sufficiency. In many areas, the growth limiting nutrient is iron and it is now known that the presence of ferritin in these phytoplankton is crucial for their ability to utilise temporarily available iron. This research will lead to a significant advance in our understanding of iron cycling processes and of the relationship between structural and functional properties of ferritins. This will also have more general impact in understanding the reactivities of iron proteins and in protein-driven biomineralisation processes.
铁对几乎所有形式的生命都是必不可少的,在生命赖以生存的许多反应中发挥着核心作用。尽管这种金属在地壳中含量非常丰富,但它在很大程度上被高度不溶的矿物“锁住”,这严重限制了它对生物的可获得性。铁的稀缺导致微生物进化出许多巧妙的机制,以获得维持生长所需的足够数量。这包括小有机分子的分泌和再吸收,这些小有机分子可以非常紧密地结合铁,从而吸收那里的铁。病原菌可以有效地从宿主的含铁蛋白中窃取铁,而这种机制的成功是能否成功感染的关键决定因素。细胞克服铁问题的另一个重要方面是它们储存铁的能力。这是通过一种叫做铁蛋白的蛋白质家族来实现的。这些分子可以被认为是具有中空中心的足球般的分子,其中数千个铁原子可以以铁矿物的形式储存--如果不是因为蛋白质外壳的溶解作用,这种矿物将形成不可溶的沉淀物。铁的储存有两个重要的功能。首先,当附近环境中的铁含量特别低时,它使微生物能够利用储备;其次,它克服了铁的潜在毒性,这种毒性源于铁对生命有用的特性:如果没有适当的控制,铁可能会导致产生活性氧物种,从而导致严重的细胞损害。在这项研究中,我们建议更好地了解来自两种截然不同的微生物的两种特定铁蛋白是如何吸收铁并协调它们的铁矿物货物的沉积,以及它们随后如何释放铁。其中之一是来自生物体大肠杆菌--一种几十年来一直是了解细胞过程的主力细菌,但它也可能是致病的。另一种铁蛋白是最近在浮游植物中发现的一种。这些生物负责海洋中二氧化碳初级生产有机分子的很大比例。此外,它们还在暂时营养充足的地区形成壮观的水华。在许多地区,限制生长的营养物质是铁,现在人们知道,这些浮游植物中存在的铁蛋白对它们利用暂时可用的铁的能力至关重要。这项研究将使我们对铁的循环过程以及铁蛋白的结构和功能性质之间的关系的理解有很大的进步。这也将对理解铁蛋白质的反应性和蛋白质驱动的生物矿化过程产生更广泛的影响。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mechanism of ferrous iron binding and oxidation by ferritin from a pennate diatom.
  • DOI:
    10.1074/jbc.m113.454496
  • 发表时间:
    2013-05-24
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Pfaffen S;Abdulqadir R;Le Brun NE;Murphy ME
  • 通讯作者:
    Murphy ME
Ferritins: furnishing proteins with iron.
Three Aromatic Residues are Required for Electron Transfer during Iron Mineralization in Bacterioferritin.
A Diatom Ferritin Optimized for Iron Oxidation but Not Iron Storage.
  • DOI:
    10.1074/jbc.m115.669713
  • 发表时间:
    2015-11-20
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Pfaffen S;Bradley JM;Abdulqadir R;Firme MR;Moore GR;Le Brun NE;Murphy MEP
  • 通讯作者:
    Murphy MEP
Bioinorganic chemistry.
生物无机化学。
  • DOI:
    10.1039/c2dt90214a
  • 发表时间:
    2013
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Raven E
  • 通讯作者:
    Raven E
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Nicolas Le Brun其他文献

Nicolas Le Brun的其他文献

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{{ truncateString('Nicolas Le Brun', 18)}}的其他基金

Iron-sulfur cluster-containing sensor regulators: mechanistic and structural studies of DNA-binding
含铁硫簇的传感器调节器:DNA 结合的机制和结构研究
  • 批准号:
    BB/V006851/1
  • 财政年份:
    2022
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
The iron-regulated control network of nutrient uptake in plants
植物养分吸收的铁调节控制网络
  • 批准号:
    BB/V014625/1
  • 财政年份:
    2021
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
New high resolution mass spectrometry facilities for macromolecules and metabolites at the University of East Anglia
东安格利亚大学新的大分子和代谢物高分辨率质谱设备
  • 批准号:
    BB/T017708/1
  • 财政年份:
    2020
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
Understanding the molecular mechanism of iron-sulfur cluster biogenesis
了解铁硫簇生物发生的分子机制
  • 批准号:
    BB/S001018/1
  • 财政年份:
    2019
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
A high sensitivity elemental mass spectrometry facility to support metallo-biology research on the Norwich Research Park
支持诺维奇研究园金属生物学研究的高灵敏度元素质谱仪
  • 批准号:
    BB/R013578/1
  • 财政年份:
    2018
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
Mechanistic and Structural Insights into NO sensing by Iron-Sulfur Cluster Regulators
铁硫簇调节器对 NO 传感的机理和结构见解
  • 批准号:
    BB/P006140/1
  • 财政年份:
    2017
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
Mechanistic studies of mitochondrial ferritin, a key player in iron mediated oxidative stress response and cellular iron metabolism
线粒体铁蛋白的机制研究,铁介导的氧化应激反应和细胞铁代谢的关键参与者
  • 批准号:
    BB/R002363/1
  • 财政年份:
    2017
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
A new pathway for iron-sulfur cluster repair
铁硫簇修复的新途径
  • 批准号:
    BB/L007673/1
  • 财政年份:
    2014
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
Advanced iron-specific spectroscopies for the study of iron-sulfur cluster transcriptional regulators
用于研究铁硫簇转录调节因子的先进铁特异性光谱
  • 批准号:
    BB/K02115X/1
  • 财政年份:
    2013
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant
Biological roles and mechanisms of nitric oxide reactions with iron-sulfur cluster transcriptional regulators
一氧化氮与铁硫簇转录调节因子反应的生物学作用和机制
  • 批准号:
    BB/J003247/1
  • 财政年份:
    2012
  • 资助金额:
    $ 43.18万
  • 项目类别:
    Research Grant

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    $ 43.18万
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