MITOGENIC REGULATION OF SKELETAL MUSCLE DIFFERENTIATION

这个项目的长期目标是了解骨骼肌 分化是有规律的。 具体目标是确定如何 某些生长因子抑制肌肉分化， 当特定的有丝分裂原 以及M-和B-肌酸激酶基因是如何被调节的 在肌肉分化末期。 本项目中使用的主要方法有：细胞培养、生长因子 受体分析，突变体选择，体细胞遗传学，基因克隆，基因 测序、基因结构分析、融合和突变基因构建， 将基因导入细胞，并分析内源和外源 基因表达。 该项目与健康有关的方面是其对 了解正常发育和基因表达。 可能的医疗 应用于发育障碍，遗传疾病， 退行性肌肉疾病，正常和肿瘤生长调节 以及涉及M-和B-肌酸的一般临床诊断程序 激酶。

The long term goal of this project is to understand how skeletal muscle differentiation is regulated. The specific aims are to determine how certain growth factors repress muscle differentiation, how proliferating myoblasts become "committed" to a post-mitotic state when specific mitogens are withdrawn, and how the M- and B-creatine kinase genes are regulated during terminal muscle differentiation. Major methodologies used in this project are: cell culture, growth factor receptor analysis, mutant selection, somatic genetics, gene cloning, gene sequencing, gene structural analysis, fusion and mutant gene construction, introduction of genes into cells, and analysis of endogenous and exogenous gene expression. Health-related aspects of the project are its contribution toward the understanding of normal development and gene expression. Possible medical applications are to developmental disorders, to genetic diseases, to degenerative muscle diseases, to normal and neoplastic growth regulation and to general clinical diagnostic procedures involving M- and B-creatine kinase.