Investigating and Exploiting Nonadride Biosynthesis for the Development of a New Generation of Herbicides

研究和利用 Nonadride 生物合成来开发新一代除草剂

• 批准号: BB/J006289/1
• 负责人: Russell Cox
• 金额: $ 56.73万
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ006289%2F1
• 关键词: Investigating; Exploiting; Nonadride; Biosynthesis; Development

Fungi make a range of useful compounds such as penicillin and anti-cholesterol drugs as well as very many other compounds with diverse uses for mankind. Some fungi make complex molecules which have useful properties as herbicides which could be used to control weeds in crop species, or plants grown as bio-fuels. The use of such herbicides from a natural source could help increase crop yields and make farming more efficient and effective. This project focuses on a class of fungal herbicides known as nonadrides. It is not known how fungi produce these compounds, but it is known that genes within the fungi control the process. These compounds are unique because they kill weeds by a mechanism which is different from all other known herbicides. This project aims to discover the genes for nonadride biosynthesis with the aim of manipulating them to produce higher amounts of nonadrides and related compounds. This will be useful for the partner organisation Syngenta who will test the compounds produced for herbicide activity. We also aim to manipulate the nonadride genes to make a range of related compounds which will also be tested as herbicides. Establishing the relationships between the chemical structures of the compounds and their herbicidal effects will allow Syngenta and other agrochemical companies to design and make more effective compounds for use in agriculture. Eventually we may be able to ferment modified fungi on large scale to produce safer, more effective herbicides. Using fermentation in fungi rather than synthesis in a chemical factory will also make the production of herbicides cheaper and more environmentally sustainable.

真菌制造一系列有用的化合物，如青霉素和抗胆固醇药物，以及许多其他对人类有不同用途的化合物。一些真菌产生复杂的分子，这些分子具有除草剂的有用特性，可以用来控制作物中的杂草，或者作为生物燃料种植的植物。使用这种天然来源的除草剂可以帮助提高作物产量，使农业更加高效。这个项目的重点是一类真菌除草剂，被称为非除草剂。目前尚不清楚真菌是如何产生这些化合物的，但已知真菌内部的基因控制着这一过程。这些化合物是独一无二的，因为它们通过一种不同于所有其他已知除草剂的机制杀死杂草。该项目旨在发现非甾体生物合成的基因，目的是操纵它们产生更多的非甾体和相关化合物。这将对合作组织先正达有用，先正达将测试所生产的化合物的除草剂活性。我们还打算操纵非除草剂基因来制造一系列相关的化合物，这些化合物也将作为除草剂进行测试。建立化合物的化学结构与其除草效果之间的关系将使先正达和其他农用化学品公司能够设计和制造更有效的农业用化合物。最终，我们可能能够大规模发酵改性真菌，以生产更安全、更有效的除草剂。在真菌中使用发酵而不是在化学工厂中进行合成也将使除草剂的生产更便宜，更环保。

项目成果

Novel nonadride, heptadride and maleic acid metabolites from the byssochlamic acid producer Byssochlamys fulva IMI 40021 - an insight into the biosynthesis of maleidrides.

• DOI: 10.1039/c5cc06988b
• 发表时间: 2015-12-14
• 期刊: Chemical communications (Cambridge, England)
• 作者: Szwalbe AJ;Williams K;O'Flynn DE;Bailey AM;Mulholland NP;Vincent JL;Willis CL;Cox RJ;Simpson TJ
• 通讯作者: Simpson TJ

Characterisation of the biosynthetic pathway to agnestins A and B reveals the reductive route to chrysophanol in fungi

Agnestin A 和 B 生物合成途径的表征揭示了真菌中大黄酚的还原途径

• DOI: 10.15488/4291
• 发表时间: 2019
• 作者: Szwalbe A

Characterisation of the biosynthetic pathway to agnestins A and B reveals the reductive route to chrysophanol in fungi.

Agnestin A 和 B 生物合成途径的表征揭示了真菌中大黄酚的还原途径。

• DOI: 10.1039/c8sc03778g
• 发表时间: 2019
• 期刊: Chemical science
• 影响因子: 8.4
• 作者: Szwalbe AJ

In silico analyses of maleidride biosynthetic gene clusters.

• DOI: 10.1186/s40694-022-00132-z
• 发表时间: 2022-02-17
• 期刊: Fungal biology and biotechnology
• 作者: Williams K;de Mattos-Shipley KMJ;Willis CL;Bailey AM
• 通讯作者: Bailey AM

Minimum Information about a Biosynthetic Gene cluster.

• DOI: 10.1038/nchembio.1890
• 发表时间: 2015-09
• 期刊: Nature chemical biology
• 影响因子: 14.8
• 作者: Medema MH;Kottmann R;Yilmaz P;Cummings M;Biggins JB;Blin K;de Bruijn I;Chooi YH;Claesen J;Coates RC;Cruz-Morales P;Duddela S;Düsterhus S;Edwards DJ;Fewer DP;Garg N;Geiger C;Gomez-Escribano JP;Greule A;Hadjithomas M;Haines AS;Helfrich EJ;Hillwig ML;Ishida K;Jones AC;Jones CS;Jungmann K;Kegler C;Kim HU;Kötter P;Krug D;Masschelein J;Melnik AV;Mantovani SM;Monroe EA;Moore M;Moss N;Nützmann HW;Pan G;Pati A;Petras D;Reen FJ;Rosconi F;Rui Z;Tian Z;Tobias NJ;Tsunematsu Y;Wiemann P;Wyckoff E;Yan X;Yim G;Yu F;Xie Y;Aigle B;Apel AK;Balibar CJ;Balskus EP;Barona-Gómez F;Bechthold A;Bode HB;Borriss R;Brady SF;Brakhage AA;Caffrey P;Cheng YQ;Clardy J;Cox RJ;De Mot R;Donadio S;Donia MS;van der Donk WA;Dorrestein PC;Doyle S;Driessen AJ;Ehling-Schulz M;Entian KD;Fischbach MA;Gerwick L;Gerwick WH;Gross H;Gust B;Hertweck C;Höfte M;Jensen SE;Ju J;Katz L;Kaysser L;Klassen JL;Keller NP;Kormanec J;Kuipers OP;Kuzuyama T;Kyrpides NC;Kwon HJ;Lautru S;Lavigne R;Lee CY;Linquan B;Liu X;Liu W;Luzhetskyy A;Mahmud T;Mast Y;Méndez C;Metsä-Ketelä M;Micklefield J;Mitchell DA;Moore BS;Moreira LM;Müller R;Neilan BA;Nett M;Nielsen J;O'Gara F;Oikawa H;Osbourn A;Osburne MS;Ostash B;Payne SM;Pernodet JL;Petricek M;Piel J;Ploux O;Raaijmakers JM;Salas JA;Schmitt EK;Scott B;Seipke RF;Shen B;Sherman DH;Sivonen K;Smanski MJ;Sosio M;Stegmann E;Süssmuth RD;Tahlan K;Thomas CM;Tang Y;Truman AW;Viaud M;Walton JD;Walsh CT;Weber T;van Wezel GP;Wilkinson B;Willey JM;Wohlleben W;Wright GD;Ziemert N;Zhang C;Zotchev SB;Breitling R;Takano E;Glöckner FO
• 通讯作者: Glöckner FO