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Rhizobium bacteroid development

根瘤菌类菌发育

基本信息

批准号：
BB/J007749/2
负责人：
Philip Poole
金额：
$ 38万
依托单位：
University of Oxford
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2013
资助国家：
英国
起止时间：
2013 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FJ007749%2F2
关键词：
Rhizobium bacteroid development

项目摘要

Bacteria are simple single celled organisms that lack the membrane bound structures found in higher cells of plants and animals. However, while bacteria may have a less complex cellular organisation they carry out a huge range of chemical reactions not found in plants and animals. Bacteria are responsible for the cycling of many nutrients such as N2 (N2 is also known as nitrogen gas and consists of two nitrogen atoms bound by a strong triple bond), which is a very inert atmospheric gas. N2 makes up 78% of the atmosphere but is very unreactive and cannot be used directly as a source of nitrogen, which is needed for amino acid, protein and DNA synthesis. However, a small number of bacteria can reduce (add hydrogen) to N2 and convert it into ammonia (NH3), which is readily incorporated into amino acids and then all the other building blocks of life, by a wide range of organisms including bacteria and plants. In many parts of the world the limitation to growth of plants, which in turn support animal life, is the supply of nitrogen as ammonia or related compounds. Since up to 65% of available nitrogen (eg ammonia) comes from bacteria this makes them essential for life on earth. Within the bacteria, most of the nitrogen is actually produced by one family known as the Rhizobiacea. This remarkable group of bacteria form a symbiotic association (both partners benefit) with plants of the legume family, that results in the formation of root nodules (on pea plants these are 2-3 mm bulbs that can easily be seen by pulling up a plant and inspecting its roots). The rhizobia are held inside the nodules where the plant provides them with an ideal environment (low O2 and lots of energy) in which they can reduce N2 to ammonia. The ammonia is supplied to the plant as its nitrogen source so this is why this is known as a symbiotic interaction. It means that the plant does need any nitrogen added to the the soil and enables rapid growth. The purpose of this research is to understand how the bacteria develop inside legume root nodules. In this reserach we use peas as our model legume. Questions include how do the bacteria grow inside plants and what factors control this process? How do the bacteria know when to switch on N2 fixation?

细菌是简单的单细胞生物，缺乏在植物和动物的高等细胞中发现的膜结合结构。然而，虽然细菌的细胞组织可能不那么复杂，但它们可以进行植物和动物中没有的大量化学反应。细菌负责许多营养物质的循环，例如N2（N2也被称为氮气，由两个氮原子通过强三键结合组成），这是一种非常惰性的大气气体。N2占大气的78%，但非常不活泼，不能直接用作氨基酸，蛋白质和DNA合成所需的氮源。然而，少数细菌可以将N2还原（添加氢）并将其转化为氨（NH3），氨很容易被包括细菌和植物在内的各种生物体纳入氨基酸，然后是所有其他生命的组成部分。在世界上许多地方，限制植物生长的因素是以氨或相关化合物的形式供应氮，而植物又支持动物的生命。由于高达65%的可用氮（如氨）来自细菌，这使得它们对地球上的生命至关重要。在细菌中，大部分氮实际上是由一个被称为根瘤菌科的家族产生的。这一显著的细菌群与豆科植物形成共生关系（双方都受益），导致根瘤的形成（在豌豆植物上，这些是2-3毫米的鳞茎，可以通过拔起植物并检查其根部而容易地看到）。根瘤菌被保存在根瘤中，植物为它们提供了一个理想的环境（低O2和大量能量），它们可以将N2还原为氨。氨作为氮源供应给植物，所以这就是为什么这被称为共生相互作用。这意味着植物确实需要任何氮添加到土壤中，并使其快速生长。本研究的目的是了解细菌是如何在豆科植物根瘤内发育的。在本研究中，我们使用豌豆作为我们的模式豆类。问题包括细菌如何在植物体内生长以及什么因素控制这一过程？细菌如何知道何时开启N2固定？

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere.

DOI：
10.1007/s11104-015-2389-5
发表时间：
2015
期刊：
PLANT AND SOIL
影响因子：
4.9
作者：
Garcia-Fraile, Paula;Seaman, Jonathan C.;Karunakaran, Ramakrishnan;Edwards, Anne;Poole, Philip S.;Downie, J. Allan
通讯作者：
Downie, J. Allan

Rhizobial Chemotaxis and Motility Systems at Work in the Soil.