Spatial and temporal mapping of the pea root secretome and its control of bacterial rhizosphere colonisation

豌豆根分泌组的时空图谱及其对细菌根际定植的控制

• 批准号: BB/K001868/1
• 负责人: Philip Poole
• 金额: $ 57.51万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK001868%2F1
• 关键词: Spatial; temporal; mapping; pea; root

Bacteria are simple single celled organisms that lack the membrane bound structures found in higher cells of plants and animals. However, while bacteria may have a less complex cellular organisation they carry out a huge range of chemical reactions not found in plants and animals. Bacteria are responsible for the cycling of many nutrients such as N2 (N2 is also known as nitrogen gas and consists of two nitrogen atoms bound by a strong triple bond), which is a very inert atmospheric gas. N2 makes up 78% of the atmosphere but is very unreactive and cannot be used directly as a source of nitrogen, which is needed for amino acid, protein and DNA synthesis. However, a small number of bacteria can reduce (add hydrogen) to N2 and convert it into ammonia (NH3), which is readily incorporated into amino acids and then all the other building blocks of life, by a wide range of organisms including bacteria and plants. In many parts of the world the limitation to growth of plants, which in turn support animal life, is the supply of nitrogen as ammonia or related compounds. Since up to 65% of available nitrogen (eg ammonia) comes from bacteria this makes them essential for life on earth. Within the bacteria, most of the nitrogen is actually produced by one family known as the Rhizobiacea. This remarkable group of bacteria form a symbiotic association (both partners benefit) with plants of the legume family, that results in the formation of root nodules (on pea plants these are 2-3 mm bulbs that can easily be seen by pulling up a plant and inspecting its roots). The rhizobia are held inside the nodules where the plant provides them with an ideal environment (low O2 and lots of energy) in which they can reduce N2 to ammonia. The ammonia is supplied to the plant as its nitrogen source so this is why this is known as a symbiotic interaction. It means that the plant does need any nitrogen added to the the soil and enables rapid growth. The purpose of this research is to understand how the bacteria (rhizobia) first associate with roots in a process called colonisation. This is a vital step if the bacteria are going to go on to elicit nodule formation by the plants and reduce N2 to ammonia. Many rhizobia with desirable argonomic features, such as the ability to fix large amounts of N2 to ammonia, are out competed for nodule formation by less desirable rhizobia. We want to understand the genetic basis for the "fitness" of some strains that allows them to out compete other strains.

细菌是简单的单细胞生物，缺乏在植物和动物的高等细胞中发现的膜结合结构。然而，虽然细菌的细胞组织可能不那么复杂，但它们可以进行植物和动物中没有的大量化学反应。细菌负责许多营养物质的循环，例如N2（N2也被称为氮气，由两个氮原子通过强三键结合组成），这是一种非常惰性的大气气体。N2占大气的78%，但非常不活泼，不能直接用作氨基酸，蛋白质和DNA合成所需的氮源。然而，少数细菌可以将N2还原（添加氢）并将其转化为氨（NH3），氨很容易被包括细菌和植物在内的各种生物体纳入氨基酸，然后是所有其他生命的构建单元。在世界上许多地方，限制植物生长的因素是以氨或相关化合物的形式供应氮，而植物又支持动物的生命。由于高达65%的可用氮（如氨）来自细菌，这使得它们对地球上的生命至关重要。在细菌中，大部分氮实际上是由一个被称为根瘤菌科的家族产生的。这一显著的细菌群与豆科植物形成共生关系（双方都受益），导致根瘤的形成（在豌豆植物上，这些是2-3毫米的鳞茎，可以通过拔起植物并检查其根部而容易地看到）。根瘤菌被保存在根瘤中，植物为它们提供了一个理想的环境（低O2和大量能量），它们可以将N2还原为氨。氨作为氮源供应给植物，所以这就是为什么这被称为共生相互作用。这意味着植物确实需要任何氮添加到土壤中，并使其快速生长。这项研究的目的是了解细菌（根瘤菌）如何在一个称为殖民化的过程中首先与根部联系起来。这是一个至关重要的步骤，如果细菌要继续诱导植物形成根瘤，并将N2还原为氨。许多具有理想的农艺学特征的根瘤菌，如将大量N2固定为氨的能力，在根瘤形成方面被不太理想的根瘤菌所竞争。我们想了解一些菌株的“适应性”的遗传基础，这些适应性使它们能够与其他菌株竞争。

项目成果

A novel biosensor to monitor proline in pea root exudates and nodules under osmotic stress and recovery.

一种新型生物传感器，用于监测渗透胁迫和恢复下豌豆根分泌物和根瘤中的脯氨酸。

• DOI: 10.1007/s11104-020-04577-2
• 发表时间: 2020
• 期刊: Plant and soil
• 影响因子: 4.9
• 作者: Rubia MI

Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere.

• DOI: 10.1007/s11104-015-2389-5
• 发表时间: 2015
• 期刊: PLANT AND SOIL
• 影响因子: 4.9
• 作者: Garcia-Fraile, Paula;Seaman, Jonathan C.;Karunakaran, Ramakrishnan;Edwards, Anne;Poole, Philip S.;Downie, J. Allan
• 通讯作者: Downie, J. Allan

Mutation of praR in Rhizobium leguminosarum enhances root biofilms, improving nodulation competitiveness by increased expression of attachment proteins.

• DOI: 10.1111/mmi.12670
• 发表时间: 2014-08
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Frederix M;Edwards A;Swiderska A;Stanger A;Karunakaran R;Williams A;Abbruscato P;Sanchez-Contreras M;Poole PS;Downie JA
• 通讯作者: Downie JA

Genome sequence of Rhizobium leguminosarum bv trifolii strain WSM1689, the microsymbiont of the one flowered clover Trifolium uniflorum.

• DOI: 10.4056/sigs.4988693
• 发表时间: 2014-06-15
• 期刊: Standards in genomic sciences
• 作者: Terpolilli J;Rui T;Yates R;Howieson J;Poole P;Munk C;Tapia R;Han C;Markowitz V;Tatiparthi R;Mavrommatis K;Ivanova N;Pati A;Goodwin L;Woyke T;Kyrpides N;Reeve W
• 通讯作者: Reeve W