PIGMENT GALLSTONES: COMPOSITION AND FORMATION

色素性胆结石：成分和形成

• 批准号: 3151016
• 负责人: ROGER DAVID SOLOWAY
• 金额: $ 19.61万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151016
• 关键词: aging; bile pigments; bilirubin; calcium carbonate; calcium phosphate; cholelithiasis; electron microscopy; glucosamine; human tissue; noninvasive diagnosis; palmitates; pathology; phosphates; polymers

The purpose of this study is to examine the pathogenesis of the two major types of pigment gallstones: (1) Black stones which contain calcium salts of bilirubinate, pigment polymers, carbonates and phosphate a well as protein and (2) bilirubinate stones which contain predominantly calcium salts of bilirubinate and palmitate and other fatty acids with a small amount of protein. We have proposed that black stones form through entrapment of calcium salts by calcium-binding proteins while bilirubinate stones form secondary to stasis and/or infection which breaks down biliary lipids into components which become insoluable through precipitation with calcium. The aims of this project are: (1) characterize, quantitate and examine the regulation of protein synthesis and secretion using 3H glucosamine incorporation into gallbladder explants from guinea pigs and from patients with pigment and cholesterol gallstones. Studies include regulation of intra- and extracellular calcium, and effects on these properties of aging, lithogenic diet and stasis. (2) To utilize the non-invasive microanalytic techniques of 2-dimensional elemental microprobe analysis and ESCA surface analysis following argon and oxygen plasma etching, coupled with scanning electron microscopy to examine stone nidation and growth. (3) To examine stone composition utilizing Fourier transform infrared spectroscopy to answer previously unanswerable questions such as: the form of bilirubin in black stones, the distribution of bile acids between the center and periphery of gallstones and the location of proteins within stones.

本研究的目的是探讨这两种主要的发病机制

项目成果

Inhibition of calcium phosphate precipitation by bile salts: a test of the Ca(2+)-buffering hypothesis.

胆汁盐抑制磷酸钙沉淀：Ca(2 ) 缓冲假说的检验。

• 发表时间: 1994
• 期刊: Journal of lipid research
• 影响因子: 6.5
• 作者: Crowther,RS;Okido,M
• 通讯作者: Okido,M

Surgical significance of dilatation of the common bile duct--with special reference to choledocholithiasis.

胆总管扩张的手术意义——特别是胆总管结石。

• DOI: 10.1007/bf02468791
• 发表时间: 1980
• 期刊: The Japanese journal of surgery
• 作者: Nagase,M;Hikasa,Y;Soloway,RD;Tanimura,H;Setoyama,M;Mukaihara,S;Kato,H
• 通讯作者: Kato,H

Interaction of bile salts with calcium hydroxyapatite: Inhibitors of apatite formation exhibit high–affinity premicellar binding

胆汁盐与羟基磷灰石钙的相互作用：磷灰石形成抑制剂表现出高亲和力的前胶束结合

• DOI: 10.1002/hep.1840160526
• 发表时间: 1992
• 期刊: Hepatology
• 影响因子: 13.5
• 作者: Suimmin Qiu;R. Soloway;R. Crowther
• 通讯作者: R. Crowther

Gallstone composition in relation to buoyancy at oral cholecystography.

胆结石成分与口腔胆囊造影浮力的关系。

• DOI: 10.1148/radiology.177.1.2399314
• 发表时间: 1990
• 期刊: Radiology
• 影响因子: 19.7
• 作者: Malet,PF;Baker,J;Kahn,MJ;Soloway,RD
• 通讯作者: Soloway,RD

Interaction of human gallbladder mucin with calcium hydroxyapatite: Binding studies and the effect on hydroxyapatite formation

• DOI: 10.1002/hep.1840210621
• 发表时间: 1995-06
• 期刊: Hepatology
• 影响因子: 13.5
• 作者: S. Qiu;G. Wen;J. Wen;R. Soloway;R. Crowther