ANGIOTENSINS IN CUTANEOUS INFLAMMATION & DISEASE

血管紧张素在皮肤炎症中的作用

• 批准号: 3152374
• 负责人: BRUCE U WINTROUB
• 金额: $ 15.86万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-07-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3152374
• 关键词: cellular immunity; chemotaxis; electron microscopy; eosinophil; fibrin; fibrinogen; histamine; humoral immunity; immunologic techniques; lymphocyte; neutrophil; pathologic process; pemphigus; peptidases; peptides; physical chemical interaction; proteolysis; vesicular skin disorder

This proposal is divided into (1) study of the contribution of mast cell and eosinophil-dependent pathways to the pathogenesis of the human cutaneous blistering disorder bullous pemphigoid, and (2) isolation, characterization, and development of specific assays for components of the human neutrophil neutral peptide generating pathway, so as to permit study of this pathway in appropriate human cutaneous disorders. Neutrophil, eosinophil and lymphocyte chemotactic factors from bullous pemphigoid blister fluid will be purified and characterized so that their targets in human skin and their cell of origin may be determined. The cellular and humoral contribution to the regulation of histamine in bullous pemphigoid blister fluid will be studied. The chemical data will be correlated with the order of pathologic alterations as determined by electron microscopic and histopathologic study of developing skin lesions. Components of the neutrophil pathway for the generation of a neutral peptide mediator and the degradation of fibrinogen and fibrin will be fully characterized. The neutral peptide product will be purified, characterized, structurally defined and synthesized in vitro. The biology of components of this pathway will be studied and specific immunoassays will be developed for quantitation of each component in complex biologic fluids and detection in tissue.

该建议分为（1）肥大细胞的贡献的研究， 嗜酸性粒细胞依赖性途径对人皮肤癌发病机制的影响 水疱性疾病大疱性类天疱疮，和（2）分离，表征， 人中性粒细胞组分特异性测定方法的建立 肽产生途径，以便允许在适当的条件下研究该途径。 人类皮肤疾病 中性粒细胞、嗜酸性粒细胞和淋巴细胞趋化 大疱性类天疱疮水疱液中的因子将被纯化和表征 从而可以确定它们在人皮肤中的靶点及其来源细胞。 大疱性结肠炎中组胺的细胞和体液调节作用 将研究类天疱疮水疱液。 化学数据将被关联 病理改变的顺序由电子显微镜确定 和发展中皮肤病变的组织病理学研究。 中性粒细胞生成中性肽途径的组分 介质和纤维蛋白原和纤维蛋白的降解将充分 表征了 中性肽产物将被纯化，表征， 结构确定并在体外合成。 生物学的组成部分， 将对这一途径进行研究，并开发特异性免疫测定法， 定量复杂生物流体中的每种组分， 组织.