REGULATION OF BROWN ADIPOSE TISSUE THERMOGENESIS

棕色脂肪组织产热的调节

• 批准号: 3152585
• 负责人: EARL S SHRAGO
• 金额: $ 4.2万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1986-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3152585
• 关键词: acyl coA; body temperature regulation; brown fat; cold temperature; environmental adaptation; guanosine diphosphate; high performance liquid chromatography; ionophores; lipid metabolism; liposomes; membrane reconstitution /synthesis; mitochondria; obesity; purine nucleotides

The hypothesis that non-shivering thermogenesis (NST), mediated by brown adipose tissue mitochondria (BATM), is linked to obesity and the regulation of body weight has been recently proposed. The molecular basis for this weight regulating ability is an occurrence of a nucleotide binding protein (NbP) within the BATM inner membrane which imparts the unique capability of controlled uncoupling of respiration from normal regulatory mechanisms, thus permitting loss of energy as heat. The metabolic regulation of the NbP, and thus NST, has not been ascertained. It is known that purine nucleotides will bind and inhibit the function of the NbP, however, no activator of the NbP function has been clearly established. The NbP has many physical similarities to the adenine nucleotide translocase (ANT), which is regulated by long chain acyl CoA thioesters. It is our hypothesis that these ligands may act in a similar fashion with the NbP. This investigation will first attempt to reconstitute the purified NbP from cold adapted hamsters and use this model to characterize the effect long chain acyl CoA's have as potential metabolic regulators. The second objective will be the study of regulatory ligand interactions with NbP isolated from the genetically obese, ob/ob mouse. The characterization of NbP regulation in the ob/ob mouse has implications in the study of human obesity as these rodents have a defective NST capacity although total NbP quantity remains unchanged. Thirdly, the BAT and subcellular fraction concentration of long chain acyl CoA's will be analyzed by high pressure liquid chromatography to determine at what potential concentrations these ligands could be acting.

假设非寒颤产热（NST），介导棕色

项目成果

Functional and morphological studies of mitochondria exposed to undecagold clusters: biologic surfaces labeling with gold clusters.

暴露于十一金簇的线粒体的功能和形态学研究：用金簇标记的生物表面。

• 发表时间: 1992
• 期刊: Scanning microscopy
• 作者: Valdivia,E;Gabel,C;Reardon,JE;CaJacob,CA;Yang,H;Wehbi,RS;Scott,GL;Frey,PA;Fahien,LA
• 通讯作者: Fahien,LA

Specific interaction of fatty acyl-CoA esters with brown adipose tissue mitochondria.

脂肪酰辅酶A酯与棕色脂肪组织线粒体的特异性相互作用。

• DOI: 10.1152/ajpendo.1985.248.6.e699
• 发表时间: 1985
• 期刊: The American journal of physiology
• 作者: Strieleman,PJ;Shrago,E
• 通讯作者: Shrago,E

Partial purification and functional reconstitution of GDP sensitive brown adipose tissue mitochondria uncoupling protein using octyl glucoside.

使用辛基葡萄糖苷对 GDP 敏感的棕色脂肪组织线粒体解偶联蛋白进行部分纯化和功能重建。

• DOI: 10.1016/s0006-291x(85)80189-3
• 发表时间: 1985
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Strieleman,PJ;Schalinske,KL;Shrago,E
• 通讯作者: Shrago,E

Fatty acid activation of the reconstituted brown adipose tissue mitochondria uncoupling protein.

脂肪酸激活重建的棕色脂肪组织线粒体解偶联蛋白。

• 发表时间: 1985
• 期刊: The Journal of biological chemistry
• 作者: Strieleman,PJ;Schalinske,KL;Shrago,E
• 通讯作者: Shrago,E