CHROMOSOMES OF HUMAN BLADDER TUMORS

人类膀胱肿瘤的染色体

• 批准号: 3163962
• 负责人: AVERY A SANDBERG
• 金额: $ 18.13万
• 依托单位: SOUTHWEST BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3163962
• 关键词: bladder neoplasm; chromosome disorders; chromosome translocation; cytogenetics; gene expression; genetic markers; human tissue; karyotype; metastasis; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplasm /cancer genetics; neoplasm /cancer invasiveness; neoplasm /cancer relapse /recurrence; neurochemistry; oncogenes; urinary bladder epithelium

The aim of the proposed research continue to include 1) the establishment of further primary (specific) chromosome changes in bladder cancer akin to those already described by us and others (e.g., 5q-/-5, +7, 9q-/-9, 11p-/illq), 2) correlation of the cytogenetic findings with a number of parameters of each case (histology, including stage and grade, marker status, course of disease) to possibly delineate subtypes of bladder cancer on the basis of the primary karyotypic change, 3) to develop the application of in-situ hybridization techniques utilizing repeat sequenced probes for the recognition of at least some of the karyotypic changes (e.g., +7, -9) in interphase nuclei of superficial bladder lesions (e.g., carcinoma-in-situ) which often cannot be examined cytogenetically and 4) molecular studies of bladder cancer, as related to the chromosome changes, e.g., gene modification or altered gene expression. To further delineate primary chromosome changes much emphasis will be put on obtaining bladder cancers with a single cytogenetic anomaly or event. Since correlations appear to exist between the primary karyotypic event and prognosis (e.g., relatively good prognosis for tumors with +7 or -9 and poor prognosis for those with 5q-, i5p, or 11p-), such studies will be extended not only to larger series of tumors with known primary chromosome changes, but also to newly established subtypes. The molecular studies will be especially important in characterizing superficial bladder lesions in which cytogenetic analyses are usually not successful and in which current predictive parameters of recurrence or invasiveness are not reliable. Particularly promising appears to be in situ hybridization techniques with appropriate probes of interphase nuclei of superficial or small lesions to demonstrate such cytogenetic changes as -7 and +9 (relatively good prognosis) or loss of the Y (poor prognosis).

拟议研究的目的继续包括：1） 确定进一步的初级（特异性）染色体变化 与我们和其他人已经描述的膀胱癌相似， (e.g., 5 q-/-5，+7，9q-/-9，11p-/illq）; 2）各基因型间的相关性 每个病例的细胞遗传学结果和一些参数 （组织学，包括阶段和等级，标志物状态， 疾病），以可能描绘膀胱癌的亚型， 第一次核型变化的基础，3）发展 重复序列原位杂交技术的应用 测序探针，用于识别至少一些 核型变化（例如，+7，-9）的间期核 膀胱病变（例如，原位癌），通常不能 对膀胱进行了细胞遗传学和分子生物学研究 癌症，与染色体变化有关，例如，基因 改变或改变基因表达。 为了进一步阐明初级染色体的变化， 将用于获得具有单一细胞遗传学的膀胱癌， 异常或事件。 由于相关性似乎存在于 初级核型事件和预后（例如，相对较好 +7或-9的肿瘤预后不良， 与5 q-，i5 p，或11 p-），这样的研究将不仅扩展到 具有已知原发染色体改变的较大系列肿瘤， 而且还与新建立的亚型有关。 分子研究将 在表征浅表膀胱时尤其重要 细胞遗传学分析通常不成功的病变 其中，当前预测复发或 入侵性是不可靠的。 特别有希望的是， 使用适当的探针进行原位杂交技术， 表面或小病灶的间期核，以显示 细胞遗传学改变为-7和+9（预后较好） 或者说，是一种不好的行为。