SOLID TUMOR CHROMOSOME ANALYSIS

实体瘤染色体分析

• 批准号: 3633021
• 负责人: AVERY A SANDBERG
• 金额: $ 3.77万
• 依托单位: ROSWELL PARK CANCER INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-06-30 至 1992-06-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3633021
• 关键词: cancer risk; chromosome disorders; human tissue; karyotype; neoplasm /cancer genetics; neoplastic cell; stainings

This laboratory will be sent fresh solid tumor specimens which they will then disaggregate and incubate under appropriate growth conditions. The laboratory personnel will then make slides from mitotically active cultures. When metaphases have been identified under the microscope, the chromosomes in them will be stained by suitable methods (primarily with Wright's stain, giemsatrypsin or quinacrine) and the individual cells will be analyzed to determine the karyotype. Photographs will be taken of good metaphase spreads, and the chromosomes in them will be studied in detail. The goal of the contract is to identify changes in chromosome number and/or structure that may be important in the etiology of specific types of solid tumors.

该实验室将送来新鲜的实体瘤标本 然后它们会分解并在适当的生长下孵化 状况。 然后实验室人员将制作载玻片 有丝分裂活跃的培养物。 当中期已 在显微镜下鉴定，其中的染色体将 用适当的方法染色（主要用赖特氏染色法， 吉姆萨胰蛋白酶或奎纳克林）和单个细胞将是 分析以确定核型。 将会拍摄照片 良好的中期扩散，其中的染色体将 详细研究了。 合同的目标是识别变更 染色体数量和/或结构可能很重要 特定类型实体瘤的病因学。