SOLID TUMOR CHROMOSOME ANALYSIS

实体瘤染色体分析

• 批准号: 3633031
• 负责人: AVERY A SANDBERG
• 金额: $ 0.45万
• 依托单位: ROSWELL PARK CANCER INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-06-30 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3633031
• 关键词: cancer risk; chromosome aberrations; chromosome disorders; human tissue; karyotype; neoplasm /cancer genetics; neoplastic cell; stainings

This laboratory will be sent fresh solid tumor specimens which they will then disaggregate and incubate under appropriate growth conditions. The laboratory personnel will then make slides from mitotically active cultures. When metaphases have been identified under the microscope, the chromosomes in them will be stained by suitable methods (primarily with Wright's stain, giemsatrypsin or quinacrine) and the individual cells will be analyzed to determine the karyotype. Photographs will be taken of good metaphase spreads, and the chromosomes in them will be studied in detail. The goal of the contract is to identify changes in chromosome number and/or structure that may be important in the etiology of specific types of solid tumors.

该实验室将发送新鲜的实体瘤标本， 然后它们将分解并在适当的生长条件下孵化 条件 然后实验室人员将从 有丝分裂活跃的培养物。 当分裂中期 在显微镜下识别，它们中的染色体将被 用合适的方法染色（主要用瑞氏染色， giemsatrypsin或奎纳克林），并且将单个细胞 分析以确定核型。 将拍摄照片 良好的中期扩散，其中的染色体将 详细研究 合同的目标是确定变更 在染色体数目和/或结构中， 特定类型实体瘤的病因学。

项目成果

Effect of lipid A-associated protein and lipid A on the expression of lipopolysaccharide activity. I. Immunological activity.

• 发表时间: 1980-07
• 期刊: Immunology
• 影响因子: 6.4
• 作者: S. Izui;D. Morrison;B. Curry;F. Dixon

Radioiodination of proteins by the use of the chloramine-T method.

• DOI: 10.1016/s0076-6879(80)70050-2
• 发表时间: 1980
• 期刊: Methods in enzymology
• 作者: P. McConahey;F. Dixon

Inhibition of T cells proliferation and SLE-like syndrome of MRL/1 mice by whole body or total lymphoid irradiation.

通过全身或全淋巴照射抑制 MRL/1 小鼠的 T 细胞增殖和 SLE 样综合征。

• 发表时间: 1980
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Theofilopoulos,AN;Balderas,R;Shawler,DL;Izui,S;Kotzin,BL;Strober,S;Dixon,FJ
• 通讯作者: Dixon,FJ

Subclass-restricted IgG polyclonal antibody production in mice injected with lipid A-rich lipopolysaccharides.

注射富含脂质 A 的脂多糖的小鼠中产生亚类限制的 IgG 多克隆抗体。

• DOI: 10.1084/jem.153.2.324
• 发表时间: 1981
• 期刊: The Journal of experimental medicine
• 作者: Izui,S;Eisenberg,RA;Dixon,FJ
• 通讯作者: Dixon,FJ

New cytogenetic subtype of renal tumors.

肾肿瘤的新细胞遗传学亚型。

• DOI: 10.1016/0165-4608(88)90301-9
• 发表时间: 1988
• 期刊: Cancer genetics and cytogenetics
• 作者: DalCin,P;Sandberg,AA;Huben,R;Li,FP;Prout,GR
• 通讯作者: Prout,GR