GROWTH FACTORS IN NORMAL AND NEOPLASTIC HEMATOPOIESIS
GROWTH FACTORS IN NORMAL AND NEOPLASTIC HEMATOPOIESIS
批准号:
3169705
负责人:
JOHN W ADAMSON
金额:
$15.24万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1982
资助国家:
美国
项目状态:
已结题
起止时间:
1982-04-01 至 1992-06-30
关键词:
RNA binding proteins cell growth regulation cellular oncology colony stimulating factor complementary DNA embryo /fetus tissue /cell culture enzyme linked immunosorbent assay feline leukemia /sarcoma virus fibroblasts gel electrophoresis gene expression genetic manipulation genetic transcription glucose 6 phosphate dehydrogenase deficiency glycosylation granulocyte hematopoiesis hematopoietic stem cells high performance liquid chromatography human subject human tissue hybridomas immunomodulators interferons interleukin 1 interleukin 3 laboratory mouse laboratory rabbit lipopolysaccharides macrophage molecular cloning molecular oncology monoclonal antibody myeloproliferative neoplasm neoplastic growth nucleic acid probes oligonucleotides point mutation protein biosynthesis thrombin transfection tumor necrosis factor alpha tumor necrosis factor beta ultracentrifugation vascular endothelium
中文摘要
研究计划的目标是描述
造血生长因子,影响增殖,
早期人类祖细胞,包括正常的和肿瘤性的。 四个领域
将被强调。 首先,我们会继续研究
调节间充质细胞释放生长因子。
这将包括对各种生长因子的北方分析
从细胞如淋巴细胞、成纤维细胞、内皮细胞
细胞和平滑肌细胞 人粒细胞探针
/巨噬细胞(GM)、粒细胞(G)、巨噬细胞(M)和多-
(白细胞介素-3)集落刺激因子(CSF)将被
就业。 各种免疫介质的作用,
将研究炎症反应,包括
白细胞介素1,脂多糖,肿瘤坏死因子,
光敏素和凝血酶。 相对转录率
的基因的CSF将被测量,以确定他们是否
在不同的介质中有不同的表达
炎症
第二,我们将探讨结构/功能的关系,
人GM-和G-CSF在蛋白质和分子水平。 为
在蛋白质水平的研究,我们将产生单克隆
人GM-和G-CSF的抗体,
用这些因子的cDNA转染的哺乳动物细胞。 在
在分子水平上,将进行位点特异性诱变,
以确定基因的哪些元件对
完整蛋白质的生物学功能。
第三,我们将研究纯化的重组GM-和
G-CSF,单独和一起,对从
正常志愿者和患者的外周血和骨髓
骨髓增生性疾病 为了评估
生长因子与祖细胞的相互作用,
细胞将在无血清条件下以低浓度生长,
条件
第四,我们将寻求克隆cDNA,
猫白血病病毒感染产生的生长因子
胚胎猫成纤维细胞。 战略将涉及减法
杂交以富集生长因子的mRNA,产生
在表达载体中的cDNA文库,以及鉴定
通过生物学测定获得有用的克隆。
英文摘要
The goals of the research program are to characterize
hematopoietic growth factors which influence the proliferation of
early human progenitors, both normal and neoplastic. Four areas
will be emphasized. First, we will continue studies of the
regulation of growth factors release form mesenchymal cells.
This will include Northern analyses of the kinds of growth factors
released from cells such as lymphocytes, fibroblasts, endothelial
cells, and smooth muscle cells. Probes for human granulocyte
/macrophage (GM), granulocyte (G), macrophage (M) and multi-
(interleukin-3) colony-stimulating factors (CSFs) will be
employed. The effects of various mediators of immune and
inflammatory responses will be studied and will include
interleukin1, lipoppolysaccharide, tumor necrosis factor,
lymphotoxin, and thrombin. Relative transcription rates
of the genes for the CSFs will be measured to determine if they
are differentially expressed by the various mediators of
inflammation.
Second, we will explore the structure/function relationship of
human GM- and G-CSF at the protein and molecular levels. For
studies at the protein level, we will generate monoclonal
antibodies to human GM- and G-CSF by purifying them from
mammalian cells transfected with cDNA for these factors. At
the molecular level, sitespecific mutagenesis will be carried out
to determine which element of the gene are important for
biological function of the intact protein.
Third, we will study the effect of purified recombinant GM- and
G-CSF, alone and together, on progenitor cells obtained from the
peripheral blood and marrow of normal volunteers and patients
with myeloproliferative disorders. To assess the nature of the
interaction of the growth factors with the progenitor cells, the
cells will be grown at low concentrations under serumfree
conditions.
Fourth, we will seek to clone the cDNA for a potentially novel
growth factor produced by feline leukemia virusinfected
embtyonic cat fibroblasts. The strategy will involve subtraction
hybridization to enrich mRNA for the growth factor, generation
of a cDNA library in an expression vector, and identification of
useful clones by biological assays.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
SMALL INSTRUMENTATION GRANT
-
批准号:2231695
-
项目类别:
-
资助金额:$2.3万
-
财政年份:1994
-
负责人:JOHN W ADAMSON
-
依托单位:
PROLIFERATION AND DIFFERENTIATION OF ISOLATED STEM CELLS
-
批准号:3365640
-
项目类别:
-
资助金额:$15.95万
-
财政年份:1991
-
负责人:JOHN W ADAMSON
-
依托单位:
PROLIFERATION AND DIFFERENTIATION OF ISOLATED STEM CELLS
-
批准号:3365642
-
项目类别:
-
资助金额:$20.85万
-
财政年份:1991
-
负责人:JOHN W ADAMSON
-
依托单位:
PROLIFERATION AND DIFFERENTIATION OF ISOLATED STEM CELLS
-
批准号:3365641
-
项目类别:
-
资助金额:$20.06万
-
财政年份:1991
-
负责人:JOHN W ADAMSON
-
依托单位:
PROLIFERATION AND DIFFERENTIATION OF ISOLATED STEM CELLS
-
批准号:2223012
-
项目类别:
-
资助金额:$21.7万
-
财政年份:1991
-
负责人:JOHN W ADAMSON
-
依托单位:
PATHOBIOLOGY OF BONE MARROW SUPPRESSION IN AIDS OR ARC
-
批准号:3242929
-
项目类别:
-
资助金额:$8.07万
-
财政年份:1989
-
负责人:JOHN W ADAMSON
-
依托单位:
PATHOBIOLOGY OF BONE MARROW SUPPRESSION IN AIDS OR ARC
-
批准号:3242930
-
项目类别:
-
资助金额:$11.09万
-
财政年份:1989
-
负责人:JOHN W ADAMSON
-
依托单位:
PATHOBIOLOGY OF BONE MARROW SUPPRESSION IN AIDS OR ARC
-
批准号:3242931
-
项目类别:
-
资助金额:$11.2万
-
财政年份:1989
-
负责人:JOHN W ADAMSON
-
依托单位:
PATHOBIOLOGY OF BONE MARROW SUPPRESSION IN AIDS OR ARC
-
批准号:3242932
-
项目类别:
-
资助金额:$3.97万
-
财政年份:1989
-
负责人:JOHN W ADAMSON
-
依托单位:
MARROW FAILURE: VIRUS-INDUCED DISEASE
-
批准号:3343026
-
项目类别:
-
资助金额:$15.34万
-
财政年份:1983
-
负责人:JOHN W ADAMSON
-
依托单位:
GROWTH FACTORS IN NORMAL AND NEOPLASTIC HEMATOPOIESIS
-
批准号:3169704
-
项目类别:
-
资助金额:$15.41万
-
财政年份:1982
-
负责人:JOHN W ADAMSON
-
依托单位:
GROWTH FACTORS IN NORMAL AND NEOPLASTIC HEMATOPOIESIS
-
批准号:3169703
-
项目类别:
-
资助金额:$14.99万
-
财政年份:1982
-
负责人:JOHN W ADAMSON
-
依托单位:
GROWTH FACTORS IN NORMAL & NEOPLASTIC HEMATOPOIESIS
-
批准号:3169701
-
项目类别:
-
资助金额:$13.36万
-
财政年份:1982
-
负责人:JOHN W ADAMSON
-
依托单位:
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:2281995
-
项目类别:
-
资助金额:$1.5万
-
财政年份:1981
-
负责人:JOHN W ADAMSON
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT
-
批准号:3516180
-
项目类别:
-
资助金额:$4.82万
-
财政年份:1979
-
负责人:JOHN W ADAMSON
-
依托单位:
BIOMEDICAL RESEARCH SUPPORT
-
批准号:3516181
-
项目类别:
-
资助金额:$2.41万
-
财政年份:1979
-
负责人:JOHN W ADAMSON
-
依托单位:
IN VITRO STUDIES OF HEMATOPOIETIC REGULATION
-
批准号:3151214
-
项目类别:
-
资助金额:$16.03万
-
财政年份:1976
-
负责人:JOHN W ADAMSON
-
依托单位:
IN VITRO STUDIES OF HEMATOPOIETIC REGULATION
-
批准号:3226368
-
项目类别:
-
资助金额:$16.27万
-
财政年份:1976
-
负责人:JOHN W ADAMSON
-
依托单位:
IN VITRO STUDIES OF HEMATOPOIETIC REGULATION
-
批准号:3226369
-
项目类别:
-
资助金额:$17.73万
-
财政年份:1976
-
负责人:JOHN W ADAMSON
-
依托单位:
IN VITRO STUDIES OF HEMATOPOIETIC REGULATION
-
批准号:3226370
-
项目类别:
-
资助金额:$18.66万
-
财政年份:1976
-
负责人:JOHN W ADAMSON
-
依托单位:
海外基金