Control of plastid biogenesis by the ubiquitin-proteasome system

泛素-蛋白酶体系统对质体生物发生的控制

• 批准号: BB/K018442/1
• 负责人: Paul Jarvis
• 金额: $ 47.71万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FK018442%2F1
• 关键词: Control; plastid; biogenesis; ubiquitin; proteasome

Chloroplasts and mitochondria are normal components of many cells - they are sub-cellular structures called organelles. Interestingly, these two organelles evolved from bacteria that were engulfed by other cells over a billion years ago, and in many ways they still resemble free-living bacteria. Chloroplasts are found in plant cells, contain the green pigment chlorophyll, and are responsible for the reactions of photosynthesis (the process that captures sunlight energy and uses it to power the activities of the cell). Since photosynthesis is the only significant mechanism of energy-input into the living world, chloroplasts are of inestimable importance, not just to plants but to all life on Earth. Actually, chloroplasts belong to a wider family of related organelles called plastids. Other members of the family are the highly-pigmented chromoplasts in ripe fruits, and etioplasts in dark-grown plants. Although plastids do contain DNA (a relic from their evolutionary past as free-living photosynthetic bacteria), and so can make some of their own proteins, most of the proteins needed to form a functional plastid are encoded on DNA in the cell nucleus; these proteins are made outside of the plastid in the cellular matrix known as the cytosol. As plastids are each surrounded by a double membrane, or envelope, that is impervious to the passive movement of proteins, this presents a significant problem. To overcome the problem, plastids evolved a sophisticated protein import apparatus, which uses energy (in the form of ATP) to drive the import of proteins from the cytosol, across the envelope, to the plastid interior. This import apparatus comprises two molecular machines: one in the outer envelope membrane called TOC (an abbreviation of "Translocon at the outer envelope membrane of chloroplasts"), and another in the inner envelope membrane called TIC. Each machine is made up of several different proteins which cooperate to ensure the efficiency of import. We work on a model plant called Arabidopsis that has many advantages for research, such as an availability of numerous mutants (each one with a mutation in a specific gene). One such mutant plant, ppi1, has a defect in a TOC gene such that plastid protein import does not work efficiently. Several years ago, we identified another mutation called sp1 (this stands for "suppressor of ppi1") that counteracts the negative effects of ppi1. The gene disrupted by sp1 (the SP1 gene) encodes a type of regulatory protein called a "ubiquitin E3 ligase". These work by labelling-up unwanted proteins and targeting them for removal. Because this control mechanism was not previously known to operate in plastids, this discovery was an important breakthrough in biology. The SP1 E3 ligase carefully controls the composition of the TOC machinery so that the right proteins are always imported (this is normally good, but in the abnormal ppi1 background it is apparently a hindrance). Such control is very important when plastids need to convert from one form to another; e.g. when dark-germinated plants emerge into the light, etioplasts must change into chloroplasts so that photosynthesis can begin. In this project we will investigate whether SP1 is important for the conversion of chloroplasts into chromoplasts in tomato fruit. If it is, then our work may have commercial, agricultural importance by enabling the manipulation of fruit ripening in crops (e.g. tomato, bell pepper, citrus). We will also study in much greater detail how SP1 and related proteins control plastid development. For example, our work may elucidate how plants respond to stresses like salinity and drought, which are major limits on crop yield across the world. Photosynthetic performance (and thus the energy available to plants for growth) is strongly affected by stress, and we suspect that SP1 is involved in this process. Thus, knowledge gained from our work may enable improved adaptation of crops to adverse environmental conditions.

叶绿体和线粒体是许多细胞的正常组成部分-它们是称为细胞器的亚细胞结构。有趣的是，这两种细胞器是从10亿年前被其他细胞吞噬的细菌进化而来的，在许多方面它们仍然类似于自由生活的细菌。叶绿体存在于植物细胞中，含有绿色色素叶绿素，并负责光合作用的反应（捕获阳光能量并利用其为细胞活动提供动力的过程）。由于光合作用是能量输入到生命世界的唯一重要机制，叶绿体具有不可估量的重要性，不仅对植物，而且对地球上的所有生命。实际上，叶绿体属于一个更广泛的家族，叫做质体。该家族的其他成员是成熟果实中高度着色的色素体和黑暗生长植物中的黄化体。虽然质体确实含有DNA（这是它们作为自由生活的光合细菌的进化历史的遗物），因此可以制造它们自己的一些蛋白质，但形成功能质体所需的大多数蛋白质都编码在细胞核中的DNA上;这些蛋白质在质体外的细胞基质（称为细胞质）中制造。由于每个质体都被双层膜或包膜包围，蛋白质的被动运动是不受影响的，这就提出了一个重要的问题。为了克服这个问题，质体进化出一种复杂的蛋白质输入装置，它使用能量（以ATP的形式）来驱动蛋白质从胞质溶胶穿过包膜进入质体内部。该进口装置包括两个分子机器：一个在被称为TOC（“叶绿体外被膜处的转位子”的缩写）的外被膜中，另一个在被称为TIC的内被膜中。每个机器都由几种不同的蛋白质组成，它们相互配合以确保导入效率。我们研究了一种名为拟南芥的模式植物，它具有许多研究优势，例如可以获得许多突变体（每一个突变体都有一个特定的基因突变）。一种这样的突变体植物ppi 1在TOC基因中具有缺陷，使得质体蛋白质输入不能有效地工作。几年前，我们发现了另一种称为sp1的突变（这代表“ppi 1抑制因子”），可以抵消ppi 1的负面影响。被sp1破坏的基因（SP1基因）编码一种称为“泛素E3连接酶”的调节蛋白。这些工作原理是标记不需要的蛋白质并将其靶向去除。由于这种控制机制以前不知道在质体中运作，这一发现是生物学上的一个重要突破。SP1 E3连接酶仔细控制TOC机制的组成，以便总是输入正确的蛋白质（这通常是好的，但在异常ppi 1背景下，它显然是一个障碍）。当质体需要从一种形式转化为另一种形式时，这种控制是非常重要的;例如，当黑暗萌发的植物出现在光照下时，黄化体必须转变为叶绿体，以便光合作用可以开始。在这个项目中，我们将调查是否SP1是重要的番茄果实中的叶绿体转化为色素体。如果是，那么我们的工作可能具有商业，农业的重要性，使作物（如番茄，甜椒，柑橘）的果实成熟的操纵。我们还将更详细地研究SP1和相关蛋白如何控制质体发育。例如，我们的工作可以阐明植物如何应对盐和干旱等胁迫，这是世界各地作物产量的主要限制。光合作用的表现（以及植物生长所需的能量）受到胁迫的强烈影响，我们怀疑SP1参与了这一过程。因此，从我们的工作中获得的知识可能使作物能够更好地适应不利的环境条件。

项目成果

The chloroplast-associated protein degradation pathway controls chromoplast development and fruit ripening in tomato

• DOI: 10.1038/s41477-021-00916-y
• 发表时间: 2021-05-01
• 期刊: NATURE PLANTS
• 影响因子: 18
• 作者: Ling, Qihua;Sadali, Najiah Mohd;Jarvis, R. Paul
• 通讯作者: Jarvis, R. Paul

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

• DOI: 10.3791/54717
• 发表时间: 2016-11-01
• 期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
• 影响因子: 1.2
• 作者: Ling, Qihua;Jarvis, Paul
• 通讯作者: Jarvis, Paul

Regulation of Chloroplast Protein Import by the Ubiquitin E3 Ligase SP1 Is Important for Stress Tolerance in Plants.

• DOI: 10.1016/j.cub.2015.08.015
• 发表时间: 2015-10-05
• 期刊: Current biology : CB
• 作者: Ling Q;Jarvis P
• 通讯作者: Jarvis P

Evolutionary, molecular and genetic analyses of Tic22 homologues in Arabidopsis thaliana chloroplasts.

• DOI: 10.1371/journal.pone.0063863
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kasmati AR;Töpel M;Khan NZ;Patel R;Ling Q;Karim S;Aronsson H;Jarvis P
• 通讯作者: Jarvis P

The ubiquitin-proteasome system regulates chloroplast biogenesis.

• DOI: 10.4161/cib.23001
• 发表时间: 2013-03-01
• 期刊: Communicative & integrative biology
• 作者: Huang W;Ling Q;Jarvis P
• 通讯作者: Jarvis P