Microtools for downstream processing

用于下游加工的微型工具

• 批准号: BB/L000997/1
• 负责人: Nicolas Szita
• 金额: $ 140.8万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL000997%2F1
• 关键词: Microtools; downstream; processing

Biocatalysis uses enzymes to enable greener production process. To fully harness the advantages of this technology, it isnecessary to understand not only the reaction steps, i.e. the steps where the desired product is produced, but also thosesteps where the desired product is separated from by-products and residual (i.e. unreacted) substrate. This typicallyrequires several purification steps. And it is often the cost of these purification steps which determine the economic viabilityof such a biocatalytic process. To rapidly understand the process and operating conditions of these purification steps -which in turn determines the cost and viability of the whole process - it is necessary to rapidly analyse different purificationstep 'candidates' and select the most efficient one(s). The more rapidly such an investigation can be conducted, the fastercritical decisions about the economic viability can be taken, and the faster one can bring a product to the market.Here, we propose to use the advances in microfabrication to create miniaturised devices which can mimic the larger onestypically used for purification, so that the different steps can be evaluated more rapidly. Miniaturisation will mean a morecost-effective use of resources to undertake the investigations. Miniaturisation will also mean that particular effects can bestudied with greater detail. We envisage for example to study the effects of how product molecules 'transition' from onesolvent (e.g. aqueous one) to another solvent (e.g. an organic one), and under what conditions they do so best, in aprocess that is called liquid-liquid extraction. We will then use this information to determine how larger systems (ie thesystems where the products will effectively be produced and purified) should be designed to improve their yield. Such animprovement in yield should make biocatalysis an even greener process and make it also economically more viable.

生物催化使用酶来实现更绿色的生产过程。为了充分利用该技术的优点，不仅需要了解反应步骤，即产生所需产物的步骤，而且还需要了解将所需产物与副产物和残余（即未反应的）底物分离的步骤。这通常需要几个纯化步骤。而且这些纯化步骤的成本通常决定了这种生物催化过程的经济可行性。为了快速了解这些纯化步骤的工艺和操作条件-这反过来又决定了整个工艺的成本和可行性-有必要快速分析不同的纯化步骤“候选者”并选择最有效的步骤。越快这样的调查可以进行，fastercritical决定的经济可行性，可以更快地把一个产品推向市场，在这里，我们建议使用的进步，在微细加工，以创建可模仿较大的onestypically用于净化设备，使不同的步骤可以更快地进行评估。小型化将意味着以最具成本效益的方式利用资源进行调查。微型化还意味着可以更详细地研究特定的效果。例如，我们设想研究产品分子如何从一种溶剂（例如水性溶剂）“过渡”到另一种溶剂（例如有机溶剂）的影响，以及在什么条件下它们在称为液-液萃取的过程中表现最佳。然后，我们将使用这些信息来确定如何设计更大的系统（即有效生产和纯化产品的系统）以提高产量。这种产量的提高应该使生物催化成为一种更加绿色的过程，并使其在经济上更加可行。

项目成果

Removal and dispersal of biofluid films by powered medical devices: Modeling infectious agent spreading in dentistry.

• DOI: 10.1016/j.isci.2021.103344
• 发表时间: 2021-11-19
• 期刊: iScience
• 影响因子: 5.8
• 作者: Eames I;D'Aiuto F;Shahreza S;Javanmardi Y;Balachandran R;Hyde M;Ng YL;Gulabivala K;Watson S;Davies H;Szita N;Khajeh J;Suvan J;Moeendarbary E
• 通讯作者: Moeendarbary E

Quantifying cell-generated forces: Poisson's ratio matters.

• DOI: 10.1038/s42005-021-00740-y
• 发表时间: 2021-11-04
• 期刊: Communications physics
• 影响因子: 5.5
• 作者: Javanmardi Y;Colin-York H;Szita N;Fritzsche M;Moeendarbary E
• 通讯作者: Moeendarbary E

Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system.

• DOI: 10.1038/s41598-017-11533-1
• 发表时间: 2017-09-21
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Dimov N;Kastner E;Hussain M;Perrie Y;Szita N
• 通讯作者: Szita N

Enzymatic synthesis of chiral amino-alcohols by coupling transketolase and transaminase-catalyzed reactions in a cascading continuous-flow microreactor system.

• DOI: 10.1002/bit.26470
• 发表时间: 2018-03
• 期刊: Biotechnology and bioengineering
• 影响因子: 3.8
• 作者: Gruber P;Carvalho F;Marques MPC;O'Sullivan B;Subrizi F;Dobrijevic D;Ward J;Hailes HC;Fernandes P;Wohlgemuth R;Baganz F;Szita N
• 通讯作者: Szita N

Microfluidic Devices as Process Development Tools for Cellular Therapy Manufacturing.

微流体装置作为细胞治疗制造的工艺开发工具。

• DOI: 10.1007/10_2021_169
• 发表时间: 2022
• 期刊: Advances in biochemical engineering/biotechnology
• 作者: Aranda Hernandez J