RADIOACTIVE LABELING OF BLOOD CELLS AND THEIR PRODUCTS

该项目旨在改进目前标记白细胞的方法 和血小板与γ-发射剂， 闪烁引起的炎性病变、内皮损伤和血栓 照相机成像。 将开发新的放射性药物，包括 8-羟基喹啉的类似物、二硫化物化合物和 双缩氨基硫脲 这些亲脂性螯合剂将被标记 99mTc、111In、197Hg、203Pb或97Ru。 连续流淘析 将使用具有特殊离心机转子的系统来收获白细胞 用Metrizamide或Stractan密度梯度离心， 用于采集血小板 分离的标记细胞的活力将被 通过重新注射到实验动物中并测量细胞 在血流中恢复和存活。 它们的功效在于证明 将在动物中评估局灶性炎性病变和血管损伤 通过伽马照相机成像和通过直接组织放射性测定。

This project seeks to improve the current methods of labeling leukocytes and platelets with gamma-emitting agents for the delineation of focal inflammatory lesions, endothelial injury and thrombi by scintillation camera imaging. New radiopharmaceuticals will be developed, including analogues of 8-hydroxyquinoline, disulphide compounds and bis-thiosemicarbazones. These lipophilic chelating agents will be labeled with 99mTc, 111In, 197Hg, 203Pb or 97Ru. A continuous flow elutriation system with a special centrifuge rotor will be used to harvest leukocytes and centrifugation with Metrizamide or Stractan density gradients will be used to harvest platelets. Viability of the isolated labeled cells will be assessed by reinjection into experimental animals and measurement of cell recovery and survival in the blood stream. Their efficacy in demonstrating focal inflammatory lesions and vascular damage will be assessed in animals by gamma camera imaging and by direct tissue radioassay.