MAGNETIC FIELD EFFECTS ON MACROPHAGES
MAGNETIC FIELD EFFECTS ON MACROPHAGES
批准号:
3180995
负责人:
Joseph D Brain
金额:
$16.08万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-12-01 至 1992-11-30
关键词:
aerosols alveolar macrophages cell migration cell motility chemotaxis cytoplasm diagnostic respiratory lavage hamsters intracellular parasitism iron oxide lysosomes magnetic field magnetic resonance imaging magnetism membrane fusion nonblood rheology particle phagocytosis photomicrography tissue /cell culture vesicle /vacuole video recording system viscosity
中文摘要
活体核磁共振(NMR)将活体组织暴露于
英文摘要
In vivo nuclear magnetic resonance (NMR) exposes living tissue to
large magnetic fields. Tissue constituents interact weakly with
magnetic fields, and the direct effects of magnetic fields on
cellular, biochemical, and genetic processes have not been
reproducibly established. However, microscopic ferromagnetic
particles be present as contaminants in the lungs and other organs.
in many occupational settings, humans inhale magnetic-particle
aerosols; a fraction of the particles are retained in the lungs and
are ingested by pulmonary macrophages. Strong magnetic fields can
cause these magnetic particles to move, rotate, and agglomerate.
The toxicology of NMR fields on cells and tissues containing
ferromagnetic particles is unknown.
Years 01 and 02 of the project demonstrated that microscopic
magnetic particles can be observed both optically and magnetically
in cells. Moreover, such particles can serve as an experimental
indicator of macrophage function by noninvasively providing
information on the motions of cell organelles. We found that for
phagocytized particles, cytoplasmic motions rotate particles and
produce detectable changes in the magnetic field. In addition,
intracellular particles can be twisted by an applied external
field, which allows measurement of cytosol viscoelasticity.
The proposed researeh will examine magnetie field effects on
particle-containing lung macrophages. In years 04-06 we will
address the following questions:
(1) Can magnetic forces overcome the motile forces generated by
the macrophage cytoplasm? Can magnetic forces restrain chemotaxis?
We will quantify the magnitude of traction forces
magnetometrically.
(2) Can motion imposed by magnet fields on magnetic-particle
containing cell organelles modulate the fusion of phagocytic and
lysosomal vesicles?
(3) Can alterations in cell organelle motions, magnetometrically
detected, be used to identify the existence and activity of an
intracellular infection (e.g., AIDS) in macrophages?
The goal is to examine how phagocytized ferromagnetic particles
modify cell function when cells are exposed to strong magnetic
fields. At the same time, we will measure the forces generated in
cell motility, we will examine the role of organelle motions in
phagolysosome processing, and we will identify changes in motion
and rheology caused by viral infection.
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Kinetics of phagocytosis and phagosome-lysosome fusion in hamster lung and peritoneal macrophages.
仓鼠肺和腹膜巨噬细胞吞噬作用和吞噬体-溶酶体融合的动力学。
DOI:
10.1002/jlb.50.3.229
发表时间:
1991
期刊:
Journal of leukocyte biology
影响因子:
5.5
作者:
[Bizal,CL, Butler,JP, Feldman,HA, Valberg,PA]
通讯作者:
Valberg,PA
DOI:
10.1083/jcb.109.5.2233
发表时间:
1989-11
期刊:
The Journal of cell biology
影响因子:
--
作者:
[Zaner KS, Valberg PA]
通讯作者:
Valberg PA
DOI:
10.1016/s0006-3495(87)83244-7
发表时间:
1987-10
期刊:
Biophysical journal
影响因子:
3.4
作者:
[P. Valberg;H. Feldman]
通讯作者:
P. Valberg;H. Feldman
Intracellular oxidant production by isolated diaphragm.
通过隔离隔膜产生细胞内氧化剂。
DOI:
10.1378/chest.97.3_supplement.97s-b
发表时间:
1990
期刊:
Chest
影响因子:
9.6
作者:
[Reid,MB, Valberg,PA]
通讯作者:
Valberg,PA
Cell organelle motions in bronchoalveolar lavage macrophages from smokers and nonsmokers.
吸烟者和非吸烟者支气管肺泡灌洗巨噬细胞中的细胞器运动。
DOI:
10.1164/ajrccm/141.5_pt_1.1272
发表时间:
1990
期刊:
The American review of respiratory disease
影响因子:
--
作者:
[Valberg,PA, Jensen,WA, Rose,RM]
通讯作者:
Rose,RM
共 6 条
METAL MIXTURES AND CHILDREN'S HEALTH
-
批准号:7392827
-
项目类别:
-
资助金额:$59.5万
-
财政年份:2004
-
负责人:Joseph D Brain
-
依托单位:
Program Enrichment
-
批准号:6999085
-
项目类别:
-
资助金额:$0.98万
-
财政年份:2004
-
负责人:Joseph D Brain
-
依托单位:
Newly Recruited Invest
-
批准号:6999089
-
项目类别:
-
资助金额:$11.81万
-
财政年份:2004
-
负责人:Joseph D Brain
-
依托单位:
Administrative Core
-
批准号:6721096
-
项目类别:
-
资助金额:$18.27万
-
财政年份:2004
-
负责人:Joseph D Brain
-
依托单位:
Manganese, Iron, Cadmium & Lead Transport from the Environment to Critical Organs
-
批准号:6968759
-
项目类别:
-
资助金额:$9.5万
-
财政年份:2004
-
负责人:Joseph D Brain
-
依托单位:
Pilot Projects
-
批准号:6721116
-
项目类别:
-
资助金额:$23.03万
-
财政年份:2004
-
负责人:Joseph D Brain
-
依托单位:
CORE--RESPIRATORY BIOLOGY AND INHALATION TOXICOLOGY
-
批准号:6577753
-
项目类别:
-
资助金额:$22.85万
-
财政年份:2002
-
负责人:Joseph D Brain
-
依托单位:
CORE--RESPIRATORY BIOLOGY AND INHALATION TOXICOLOGY
-
批准号:6495606
-
项目类别:
-
资助金额:$22.85万
-
财政年份:2001
-
负责人:Joseph D Brain
-
依托单位:
CORE--RESPIRATORY BIOLOGY AND INHALATION TOXICOLOGY
-
批准号:6442276
-
项目类别:
-
资助金额:$28.07万
-
财政年份:2001
-
负责人:Joseph D Brain
-
依托单位:
CORE--RESPIRATORY BIOLOGY AND INHALATION TOXICOLOGY
-
批准号:6301235
-
项目类别:
-
资助金额:$13.27万
-
财政年份:2000
-
负责人:Joseph D Brain
-
依托单位:
CORE--RESPIRATORY BIOLOGY AND INHALATION TOXICOLOGY
-
批准号:6217583
-
项目类别:
-
资助金额:$13.27万
-
财政年份:1999
-
负责人:Joseph D Brain
-
依托单位:
CORE--RESPIRATORY BIOLOGY AND INHALATION TOXICOLOGY
-
批准号:6105983
-
项目类别:
-
资助金额:$13.27万
-
财政年份:1999
-
负责人:Joseph D Brain
-
依托单位:
CORE--RESPIRATORY BIOLOGY AND INHALATION TOXICOLOGY
-
批准号:6270891
-
项目类别:
-
资助金额:$11.84万
-
财政年份:1998
-
负责人:Joseph D Brain
-
依托单位:
HSPH NIEHS Center for Environmental Health
-
批准号:7217409
-
项目类别:
-
资助金额:$167.39万
-
财政年份:1997
-
负责人:Joseph D Brain
-
依托单位:
HSPH NIEHS Center for Environmental Health
-
批准号:6891904
-
项目类别:
-
资助金额:$171.75万
-
财政年份:1997
-
负责人:Joseph D Brain
-
依托单位:
RESPIRATORY BIOLOGY AND INHALATION TOXICOLOGY STUDIES
-
批准号:6239319
-
项目类别:
-
资助金额:$21.69万
-
财政年份:1997
-
负责人:Joseph D Brain
-
依托单位:
HSPH NIEHS Center for Environmental Health
-
批准号:7048578
-
项目类别:
-
资助金额:$165.65万
-
财政年份:1997
-
负责人:Joseph D Brain
-
依托单位:
HSPH NIEHS Center for Environmental Health
-
批准号:6720817
-
项目类别:
-
资助金额:$171.25万
-
财政年份:1997
-
负责人:Joseph D Brain
-
依托单位:
SHORT-TERM RESEARCH TRAINING FOR MINORITY STUDENTS
-
批准号:3545922
-
项目类别:
-
资助金额:$1.54万
-
财政年份:1991
-
负责人:Joseph D Brain
-
依托单位:
Short-Term Research Training for Minority Students
-
批准号:7037613
-
项目类别:
-
资助金额:$5.08万
-
财政年份:1991
-
负责人:Joseph D Brain
-
依托单位:
海外基金