IMMUNOCYTOCHEMISTRY OF PROSTATIC ACID PHOSPHATASE

先前的研究已经产生了一种前列腺酸特异性抗体 磷酸酶带2a。 这项建议包括努力生产 抗酸性磷酸酶带4的单克隆抗体。 抗体会 用于这些酶的电子显微镜免疫细胞化学研究 在前列腺以及非前列腺细胞，以了解正常的 前列腺上皮细胞的分泌机制，并寻找 前列腺癌细胞的改变。 用特异性抗体显示同工酶2在 前列腺增生和腺癌。 前列腺增生 同工酶2主要分布在高尔基体池中， 粗面内质网中少见柱状上皮细胞颗粒。 一些 同工酶2可见于质膜，并被释放到细胞内。 腺腔 在前列腺腺癌中发现同工酶2， 柱状上皮细胞和基底细胞中。 同工酶2是 可见于粗面内质网池、高尔基体和分泌颗粒。 一些同功酶2见于间质间隙，偶尔见于 毛细血管的内腔。 这些发现表明， 在增生状态下，同工酶2在粗面内质网中合成并转运 到高尔基体，并转移到分泌颗粒， 如正常前列腺所预期的那样胞吐进入体腔。 前列腺 腺癌还分泌另外的同工酶2到基底膜 叶片，从那里它被运送到血管系统。 （五）

Previous studies have produced an antibody specific to prostatic acid phosphatase band 2a. This proposal includes an effort to produce monoclonal antibody to acid phosphatase band 4. The antibodies will be used for electron microscopic-immunocytochemical studies of these enzymes in prostatic as well as non-prostatic cells in order to learn the normal secretory mechanism of the prostatic epithelial cells, and look for alterations in prostatic cancer cells. Specific antibody was used to demonstrate the location of isoenzyme 2 in hyperplastic and prostatic adenocarcinoma. In hyperplastic prostate isoenzyme 2 was observed in the cisternae of Golgi apparatus and secretory granules of the columnar epithelia cells, and rarely in RER. Some isoenzyme 2 was seen in the plasma membrane and discharged into the glandular lumen. In the prostatic adenocarcinoma isoenzymne 2 was found in both columnar epithelial cells and in the basal cells. Isoenzyme 2 was seen in the cisternae of rough ER, Golgi appartus and secretory granules. Some isoenzyme 2 was seen in the interstitial spaces and occasionally in the lumen of the capillary. These findings suggest that in the hyperplastic state, isoenzyme 2 is synthesized in the RER and transported to the Golgi apparatus, and tranferred to the secretory granules for exocytosis into thelumen as expected for normal prostate. In prostatic adenocarcinoma additional isoenzyme 2 was secreted also into the basal lamina, from where it is transported to the vascular system. (5)