IMMUNOCYTOCHEMISTRY OF PROSTATIC ACID PHOSPHATASE

先前的研究已经产生了一种针对前列腺酸的特异性抗体 磷酸酶带 2a。 该提案包括努力生产 酸性磷酸酶带 4 的单克隆抗体。抗体将是 用于这些酶的电子显微镜免疫细胞化学研究 在前列腺和非前列腺细胞中，以了解正常情况 前列腺上皮细胞的分泌机制，并寻找 前列腺癌细胞的改变。 使用特异性抗体来证明同工酶 2 的位置 增生性和前列腺腺癌。 在增生性前列腺中 在高尔基体和分泌池中观察到同工酶 2 柱状上皮细胞的颗粒，很少见于 RER。 一些 同工酶 2 出现在质膜中并排出到 腺腔。 在前列腺癌中发现同工酶2 柱状上皮细胞和基底细胞。 同工酶 2 是 见于粗糙内质网池、高尔基体和分泌颗粒。 一些同工酶 2 可见于间隙空间，偶尔也可见于 毛细血管的管腔。 这些发现表明，在 增生状态下，同工酶2在RER中合成并转运 到高尔基体，并转移到分泌颗粒 正如正常前列腺所预期的，胞吐作用进入管腔。 在前列腺 腺癌额外的同工酶2也被分泌到基底细胞中 椎板，从那里转运到血管系统。 (5)

Previous studies have produced an antibody specific to prostatic acid phosphatase band 2a. This proposal includes an effort to produce monoclonal antibody to acid phosphatase band 4. The antibodies will be used for electron microscopic-immunocytochemical studies of these enzymes in prostatic as well as non-prostatic cells in order to learn the normal secretory mechanism of the prostatic epithelial cells, and look for alterations in prostatic cancer cells. Specific antibody was used to demonstrate the location of isoenzyme 2 in hyperplastic and prostatic adenocarcinoma. In hyperplastic prostate isoenzyme 2 was observed in the cisternae of Golgi apparatus and secretory granules of the columnar epithelia cells, and rarely in RER. Some isoenzyme 2 was seen in the plasma membrane and discharged into the glandular lumen. In the prostatic adenocarcinoma isoenzymne 2 was found in both columnar epithelial cells and in the basal cells. Isoenzyme 2 was seen in the cisternae of rough ER, Golgi appartus and secretory granules. Some isoenzyme 2 was seen in the interstitial spaces and occasionally in the lumen of the capillary. These findings suggest that in the hyperplastic state, isoenzyme 2 is synthesized in the RER and transported to the Golgi apparatus, and tranferred to the secretory granules for exocytosis into thelumen as expected for normal prostate. In prostatic adenocarcinoma additional isoenzyme 2 was secreted also into the basal lamina, from where it is transported to the vascular system. (5)