FUNCTION OF ASCORBATE PEROXIDASE IN THE EYE

细胞氧化反应产生的自由基的毒性是 众所周知。 在眼组织中，频繁暴露在光线下会造成 自由基的额外来源。 过氧化物酶是主要的酶系统 视网膜色素上皮细胞，防止 H2O2 积聚。 细节 d 视网膜色素上皮中这种酶的生化性质及其 其他组织的过氧化物酶的身份尚不清楚。 现在的 具体目的是从视网膜色素中纯化主要的过氧化物酶 上皮细胞，将其特性与红细胞、粒细胞、 并产生针对纯化的过氧化物酶的特异性抗体。 抗体 y 将用于可视化其在眼组织中的位置。

The toxicity of free radicals produced in cellular oxidative reactions is well known. In eye tissues, the frequent exposure to light poses an additional source of free radical. Peroxidase is the major enzyme system i the retinal pigment epithelium to prevent accumulation of H2O2. The detail d biochemical nature of this enzyme in the retinal pigment epithelium and its identity of the peroxidase of other tissues is not known. The present specific aim is to purify the major peroxidase from the retinal pigment epithelium, compare its properties with that of erythrocytes, granulocytes, and to produce a specific antibody for the purified peroxidase. The antibo y will be used to visualize its location in the eye tissues.