FUNCTION OF ASCORBATE PEROXIDASE IN THE EYE

细胞氧化反应中产生的自由基的毒性是 人所共知。在眼睛组织中，频繁暴露在光线下会造成 额外的自由基来源。过氧化物酶是主要的酶系统I。 保护视网膜色素上皮，防止过氧化氢积聚。细节 D 视网膜色素上皮中该酶的生化性质及其意义 其他组织的过氧化物酶的特性尚不清楚。现在 具体目的是从视网膜色素中提纯主要的过氧化物酶 上皮细胞，与红细胞、粒细胞、 并为纯化的过氧化物酶产生特异性抗体。反抗剂 是 将被用来可视化它在眼睛组织中的位置。

The toxicity of free radicals produced in cellular oxidative reactions is well known. In eye tissues, the frequent exposure to light poses an additional source of free radical. Peroxidase is the major enzyme system i the retinal pigment epithelium to prevent accumulation of H2O2. The detail d biochemical nature of this enzyme in the retinal pigment epithelium and its identity of the peroxidase of other tissues is not known. The present specific aim is to purify the major peroxidase from the retinal pigment epithelium, compare its properties with that of erythrocytes, granulocytes, and to produce a specific antibody for the purified peroxidase. The antibo y will be used to visualize its location in the eye tissues.