DIETARY FAT AND HUMAN BREAST CARCINOMA GROWTH

尽管大量的研究活动都是针对 饲料脂肪在癌性啮齿动物乳腺生长中的作用 现在重要的是确定人类乳房是否致癌 组织对饮食脂肪的反应已经在啮齿动物身上得到了充分的证明。 尽管在人类群体中进行了大量的流行病学研究 提供的证据表明，富含脂肪的饮食可能会增加 关于乳腺癌的发展，最近的一些研究已经 严重质疑这段关系。因此，到目前为止，我们只有 将饮食脂肪与人类乳腺癌发生联系起来的流行病学数据。 在这项研究提案中，我们打算检查这一潜在的重要 通过确定膳食脂肪是否会影响生长来确定两者之间的关系 人乳腺癌变上皮细胞在细胞内的突起 雌性裸鼠。更具体地说，以下四个目标 将会被追查。 1.膳食脂肪的数量和类型是否会影响癌症的生长 人乳腺细胞(激素反应性MCF-7、T47-D、ZR75-1细胞系、 激素不敏感的Hs578T、MX-1细胞株)？ 2.高脂饮食对肿瘤生长有刺激作用吗 人类乳腺细胞取决于一种随意类型的喂养模式？ 3.鱼油饮食是否对癌症的生长有抑制作用 人乳腺细胞癌脂蓄积增加的功能 过氧物？ 4.膳食脂肪的数量和类型是否影响儿童的生长 通过改变癌细胞增殖而致癌的人乳腺细胞 和/或通过改变癌细胞丢失(细胞溶解)？ 总而言之，我们认为这项研究中概述的研究项目 建议是我们过去研究的合乎逻辑和重要的扩展；这些 研究不仅具有概念性和机械性的重要性，而且在 此外，解决当前一个重要的实际问题。

Although a vast amount of research activities have been directed toward the role of dietary fat in growth of the carcinomatous rodent mammary gland, it is now important to determine whether or not carcinomatous human breast tissues respond to dietary fat as has been amply demonstrated in rodents. Although numerous epidemiological studies in human populations have provided evidence that diets rich in fat may increase the risk for the development of breast cancer, a number of more recent studies have seriously questioned this relationship. Thus, to date, we have only epidemiological data to link dietary fat and human breast cancerigenesis. In this research proposal, we intend to examine this potentially important relationship by determining whether or not dietary fat can affect growth processes of carcinomatous human breast epithelial cells maintained in female athymic nude mice. More specifically, the following four objectives will be pursued. 1.Can the amount and type of dietary fat affect the growth of carcinomatous human breast cells (hormone responsiveness MCF-7, T47-D, ZR75-1 cell lines, hormone nonresponsive Hs578T, MX-1 cell lines)? 2.Is the stimulatory effect of a high fat diet on growth of carcinomatous human breast cells contingent upon an ad libitum type of feeding pattern? 3.Is the inhibitory effect of a fish oil diet on growth of carcinomatous human breast cells a function of increased accumulation of carcinoma lipid peroxides? 4.Does the amount and type of dietary fat effect the growth of carcinomatous human breast cells by altering carcinoma cell proliferation and/or by altering carcinoma cell loss (cytolysis)? In summary, we feel that the research projects outlined in this research proposal are a logical and important extension of our past studies; these studies are not only of conceptual and of mechanistic importance but, in addition, address an important current practical issue.