DIETARY FAT AND HUMAN BREAST CARCINOMA GROWTH

虽然大量的研究活动已经指向 膳食脂肪在致癌啮齿类动物乳腺生长中的作用， 现在重要是确定人类乳腺癌是否 组织对膳食脂肪有反应，这在啮齿动物中已得到充分证实。 尽管在人群中进行的许多流行病学研究 提供的证据表明，富含脂肪的饮食可能会增加 乳腺癌的发展，一些最近的研究已经 严重质疑这种关系。 到目前为止，我们只有 流行病学数据将膳食脂肪与人类乳腺癌发生联系起来。 在这项研究计划中，我们打算研究这一潜在的重要问题， 通过确定膳食脂肪是否会影响生长 癌性人乳腺上皮细胞的过程维持在 雌性无胸腺裸鼠。 具体而言，以下四个目标 将被追捕。 1.膳食脂肪的数量和类型会影响癌细胞的生长吗 人乳腺细胞（激素反应性MCF-7，T47-D，ZR 75 -1细胞系， Hs 578 T，MX-1细胞系）？ 2.高脂饮食对肿瘤生长的刺激作用 人类乳腺细胞取决于自由进食模式？ 3.鱼油饮食对癌细胞生长的抑制作用 人乳腺细胞癌脂质积累增加的功能 过氧化物？ 4.饮食中脂肪的数量和类型是否会影响 通过改变癌细胞增殖来诱导人乳腺癌细胞 和/或通过改变癌细胞损失（细胞溶解）？ 总之，我们认为，本研究中概述的研究项目 建议是我们过去研究的逻辑和重要扩展;这些 研究不仅具有概念和机械的重要性， 此外，解决当前一个重要的实际问题。

Although a vast amount of research activities have been directed toward the role of dietary fat in growth of the carcinomatous rodent mammary gland, it is now important to determine whether or not carcinomatous human breast tissues respond to dietary fat as has been amply demonstrated in rodents. Although numerous epidemiological studies in human populations have provided evidence that diets rich in fat may increase the risk for the development of breast cancer, a number of more recent studies have seriously questioned this relationship. Thus, to date, we have only epidemiological data to link dietary fat and human breast cancerigenesis. In this research proposal, we intend to examine this potentially important relationship by determining whether or not dietary fat can affect growth processes of carcinomatous human breast epithelial cells maintained in female athymic nude mice. More specifically, the following four objectives will be pursued. 1.Can the amount and type of dietary fat affect the growth of carcinomatous human breast cells (hormone responsiveness MCF-7, T47-D, ZR75-1 cell lines, hormone nonresponsive Hs578T, MX-1 cell lines)? 2.Is the stimulatory effect of a high fat diet on growth of carcinomatous human breast cells contingent upon an ad libitum type of feeding pattern? 3.Is the inhibitory effect of a fish oil diet on growth of carcinomatous human breast cells a function of increased accumulation of carcinoma lipid peroxides? 4.Does the amount and type of dietary fat effect the growth of carcinomatous human breast cells by altering carcinoma cell proliferation and/or by altering carcinoma cell loss (cytolysis)? In summary, we feel that the research projects outlined in this research proposal are a logical and important extension of our past studies; these studies are not only of conceptual and of mechanistic importance but, in addition, address an important current practical issue.