GROWTH AND DIFFERENTIATION OF HUMAN BREAST

简而言之，这项研究计划的具体目标和方法是 以下：i)定义最大生长所需的(荷尔蒙) 在裸鼠体内保存的正常人类乳房组织。a) 人体正常乳腺组织的切片将被移植到S.C.至和 在激素治疗的裸鼠中维持(1-9个月)。B)增长 人乳房组织移植物中的表观分子的含量将由：1) ~3H-胸腺嘧啶核苷掺入DNA的放射自显影分析(标记 指标)和2)整架形态分析。Ii)界定 正常乳汁分化(哺乳)所需(激素) 裸鼠体内保存的人乳房组织。A)切片 人体正常的乳房组织将被移植到S.C.至并维护 荷尔蒙处理的裸鼠(1-9个月)。B)分泌物 人类乳腺组织移植物的(哺乳期)将通过以下方式进行评估：1) α-乳铁蛋白放射免疫法和2)组织学分析。在 提出研究建议，我们将努力证明人类正常的乳房 组织，当移植到激素治疗的裸鼠身上时，可以 精确地发挥作用，就像它在人类中所做的那样，即生长和 乳酸盐。如果能够证明这一点，那么我们将第一次 时间，一个体外的体内模型来研究发育，生长， 人类乳房的分化和最终的肿瘤发生。

Concisely, the specific aims and methodology of this research proposal are the following: I) To define the requirements (hormonal) for maximum growth of normal human breast tissues maintained in the athymic nude mouse. a) Slices of normal human breast tissues will be transplanted s.c. to and maintained (1-9 months) in hormone treated athymnic nude mice. b) Growth of the epitehlium in the human breast tissue grafts will be asessed by: 1) autoradiographic analysis of 3H-thymidine incorporation into DNA (labelling index) and 2) whole-mount morphological analysis. II) To define the requirements (hormonal) for macimum differentiation (lactation) of normal human breast tissues maintained in the athymic nude mouse. a) Slices of normal human breast tissues will be transplanted s.c. to and maintained (1-9 months) in hormone treated athymic nude mice. b) Secretion (lactation) of the human breast tissue grafts will be assessed by: 1) Alpha-lactalblmin radioimmunoassay and 2) histological analysis. In the proposed reasearch, we will strive to demonstrate that normal human breast tissues, when transplanted to hormone-treated athymic nude mouse, can function precisely, as it does in the human being, i.e., to grow and lactate. If this can be demonstrated, we will then have for the first time, an ex vivo in vivo model to study development, growth, differentiation and ultimately tumorigenesis of the human breast.