FATS IN RODENT DIETS AND MITOGENIC HYDROXYLATED METABOLITES IN MAMMARY TISSUE

我们开发了GC-MS方法，通过该方法定量9-HODE （羟基辛二烯酸）和13-HODE在啮齿动物乳腺和 在小鼠乳腺中发现高水平的9-HODE和13-HODE 食用高玉米油（20%重量）的饮食（ 高乳腺致瘤性），在低剂量饮食的小鼠中水平较低， 玉米油（5%）（一种不易引起乳腺肿瘤的饮食）和低 食用牛脂（20%）的小鼠腺体中的水平（ 也是乳房致瘤性差的）。 9-HODE和 13-这些动物乳腺中的HODE可能是 酶促过程（脂氧合酶）和/或非酶促过程（自由基） 流程. 这些亚油酸的氧化产物 刺激细胞增殖过程是未知。

We have developed GC-MS methodology by which to quantitate 9-HODE (hydroxyoctadienoic acid) and 13-HODE in the rodent mammary gland and found high levels of 9-HODE and 13-HODE in the mammary glands of mice consuming a diet high in corn oil (20% by weight) (a diet that is highly mammary tumorigenic), low levels in mice consuming a diet low in corn oil (5%) (a diet that is poorly mammary tumorigenic) and low levels in glands of mice consuming beef tallow (20%) (a diet that is also poorly mammary tumorigenic). The increased levels of 9-HODE and 13-HODE in the mammary glands of these animals could be a result of enzymatic processes (lipoxygenase) and/or non-enzymatic (free radical) processes. How these oxidative products of linoleic acid might stimulate cell proliferation processes is not known.