FATS IN RODENT DIETS AND MITOGENIC HYDROXYLATED METABOLITES IN MAMMARY TISSUE

啮齿动物饮食中的脂肪和乳腺组织中的有丝分裂羟基化代谢物

• 批准号: 6258873
• 负责人: CLIFFORD W WELSCH
• 金额: $ 0.01万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6258873
• 关键词: biological products; biomedical resource; proteins; skeletal system; technology /technique development

We have developed GC-MS methodology by which to quantitate 9-HODE (hydroxyoctadienoic acid) and 13-HODE in the rodent mammary gland and found high levels of 9-HODE and 13-HODE in the mammary glands of mice consuming a diet high in corn oil (20% by weight) (a diet that is highly mammary tumorigenic), low levels in mice consuming a diet low in corn oil (5%) (a diet that is poorly mammary tumorigenic) and low levels in glands of mice consuming beef tallow (20%) (a diet that is also poorly mammary tumorigenic). The increased levels of 9-HODE and 13-HODE in the mammary glands of these animals could be a result of enzymatic processes (lipoxygenase) and/or non-enzymatic (free radical) processes. How these oxidative products of linoleic acid might stimulate cell proliferation processes is not known.

我们开发了GC-MS方法，通过该方法定量9-HODE （羟基辛二烯酸）和13-HODE在啮齿动物乳腺和 在小鼠乳腺中发现高水平的9-HODE和13-HODE 食用高玉米油（20%重量）的饮食（ 高乳腺致瘤性），在低剂量饮食的小鼠中水平较低， 玉米油（5%）（一种不易引起乳腺肿瘤的饮食）和低 食用牛脂（20%）的小鼠腺体中的水平（ 也是乳房致瘤性差的）。 9-HODE和 13-这些动物乳腺中的HODE可能是 酶促过程（脂氧合酶）和/或非酶促过程（自由基） 流程. 这些亚油酸的氧化产物 刺激细胞增殖过程是未知。