CAFFEINE & EXPERIMENTAL MAMMARY GLAND TUMORIGENESIS

咖啡因(1，3，7-三甲基黄嘌呤)具有可疑的区别 是北美使用最广泛的精神药物。 甲基化的嘌呤最常被消费在可乐、茶、 巧克力和许多处方药和非处方药。 尽管这种药物的消费无处不在，但我们仍然没有 知道药物是否是重要的贡献 “生活方式”因素在恶性疾病发生中的作用。就是这样 人类乳腺肿瘤(良性和恶性)病例； 关于是否摄入咖啡因仍存在争议 对人类乳房肿瘤的发生有贡献。 在过去的三年里，我们的研究提供了证据表明咖啡因 饮用(通过饮用水)确实会产生影响(抑制和 增强)实验动物的乳腺肿瘤发生 依赖于咖啡因的时间跨度的现象 治疗，使用的咖啡因剂量和动物模型检查。 在这项研究提案中，我们打算进一步研究 咖啡因在实验动物(大鼠和小鼠)乳腺中的含量 肿瘤发生学。 具体来说，我们打算： 1)进一步评估咖啡因在DMBA诱导的大鼠中的作用 乳腺肿瘤的发生和，此外，检查的作用 咖啡因在MNU诱导的大鼠乳腺癌模型中的作用 妊娠和激素诱导的小鼠乳腺癌模型。 此外，我们打算： 2)检测咖啡因对正常乳腺的影响 未成熟和成熟小鼠的发育生长过程和 3)测定咖啡因对促乳素的影响 幼年和成熟大鼠的分泌物。 这些研究旨在扩大我们目前对 咖啡因对正常和肿瘤性乳腺的影响 发展增长过程，并提供对 药物影响这些过程的机制。

Caffeine (1,3,7-trimethylxanthine) holds the dubious distinction of being the most widely used psychotropic drug in North America. The methylated purine is most often consumed in colas, teas, chocolates and numerous prescription and non-prescription drugs. Despite the ubiquitous consumption of the drug, we still do not know whether or not the drug is an important contributing "lifestyle" factor in the genesis of malignant disease. Such is the case with human breast tumors (benign and malignant); controversy still exists as to whether or not caffeine consumption contributes to human breast tumorigenesis. During the past 3 years, our studies provide evidence that caffeine consumption (via the drinking water) can indeed effect (inhibit and enhance) mammary gland tumorigenesis in experimental animals, a phenomenon that is dependent upon the time-span of caffeine treatment, the dose of caffeine used and the animal model examined. In this research proposal, we intend to further examine the role of caffeine in experimental animal (rat and mouse) mammary gland tumorigenesis. Specifically, we intend to: 1) further evaluate the role of caffeine in DMBA-induced rat mammary gland tumorigenesis and, in addition, examine the role of caffeine in the MNU-induced rat mammary carcinoma model and in the pregnancy- and hormone-induced mouse mammary carcinoma model. In addition, we intend to: 2) examine the influence of caffeine on normal mammary gland developmental growth processes in immature and mature mice and 3) determine the influence of caffeine on mammotropic hormone secretion in immature and mature rats. These studies are designed to extend our current understanding of the influence of caffeine on normal and neoplastic mammary gland developmental growth processes and provide insight into the mechanism by which the drug influences these processes.