NeuroChip: A microfluidic sensor for electrophysiological recording of a nematode neural network

NeuroChip：一种用于线虫神经网络电生理记录的微流体传感器

• 批准号: BB/L02439X/1
• 负责人: Lindy Holden-Dye
• 金额: $ 19.54万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL02439X%2F1
• 关键词: NeuroChip; microfluidic; sensor; electrophysiological; recording

Pharmaceutical and agrochemical industries have an increasing need for cost effective experimental platforms for compound screening. We have invented a device to screen compounds for effects on the nervous system using the microscopic nematode worm C. elegans. We liken it to a worm 'EEG'. It exploits the cost effectiveness of using an invertebrate model whilst providing an exquisite level of detail and resolution on drug action. The device is a microfluidic chamber, 'NeuroChip'. It traps the worm, records the activity of a neural circuit in its head and applies compounds through a system of microvalves. This has particular relevance for the Crop Protection and Animal Health industries as the nervous system is a proven target for the majority of compounds that are currently deployed as nematicides and antiparasitics. Moreover, it provides the potential to deliver a neurotoxicological and biological activity profile for compounds that are of interest to the pharmaceutical industry.

制药和农化行业越来越需要具有成本效益的化合物筛选实验平台。我们发明了一种装置来筛选化合物对神经系统的影响，使用的是微小的线虫线虫。我们把它比作蠕虫的脑电图。它利用了使用无脊椎动物模型的成本效益，同时提供了药物作用的精细细节和分辨率。该设备是一个微流体室，“神经芯片”。它捕获蠕虫，记录其头部神经回路的活动，并通过微阀系统施用化合物。这与作物保护和动物保健行业特别相关，因为神经系统是目前作为杀线虫剂和抗寄生虫剂使用的大多数化合物的已证实目标。此外，它还为制药行业感兴趣的化合物提供了神经毒理学和生物活性概况的潜力。

项目成果

STYLETCHIP: A MICROFLUIDIC DEVICE FOR RECORDING HOST INVASION BEHAVIOR AND FEEDING OF PLANT PARASITIC NEMATODES.

STYLETCHIP：一种用于记录宿主入侵行为和植物寄生线虫喂养的微流体装置。

• 发表时间: 2014
• 期刊: JOURNAL OF NEMATOLOGY
• 影响因子: 1.3
• 作者: Hu C.

Chemical activation of a food deprivation signal extends lifespan.

• DOI: 10.1111/acel.12492
• 发表时间: 2016-10
• 期刊: Aging cell
• 影响因子: 7.8
• 作者: Lucanic M;Garrett T;Yu I;Calahorro F;Asadi Shahmirzadi A;Miller A;Gill MS;Hughes RE;Holden-Dye L;Lithgow GJ
• 通讯作者: Lithgow GJ