13TSB_TIBio: Genomes and synthetic biology for development of novel pest control technologies

13TSB_TIBio：用于开发新型害虫防治技术的基因组和合成生物学

• 批准号: BB/M001512/1
• 负责人: Alistair Darby
• 金额: $ 11.79万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM001512%2F1
• 关键词: 13TSB_TIBio; Genomes; synthetic; biology; development

Oxitec is the world leader in application of synthetic biology to control of pest insects. Oxitec's RIDL(R) technology relies on release of mass-reared genetically sterile (transgenic) male insects that mate with their wild pest counterparts, thereby causing a drop in population. RIDL efficacy is proven against Ae. aegypti, reducing wild populations in the Caribbean and Brazil by 80-96%. A costly aspect of current technology is the means of selecting males only for release, important as the females blood-feed and transmit disease. This project aims to develop a novel method of producing male-only cohorts of RIDL mosquitoes ('genetic sexing') that would both markedly reduce costs and cement Oxitec's leadership in the field. Next-generation sequencing datasets will be analysed to help develop new RIDL traits in Ae. aegypti mosquitoes, to increase the efficiency of applying our technology in the field. Development of targeted bioinformatics methodology, and successful engineering of a novel genetic sexing technique, will provide a pipeline for development of new RIDL traits in other pests important for public health and agriculture.

Oxitec是将合成生物学应用于害虫控制的世界领导者。Oxitec的RIDL（R）技术依赖于释放大量饲养的遗传不育（转基因）雄性昆虫，这些昆虫与野生害虫交配，从而导致种群数量下降。RIDL对Ae的疗效已得到证实。埃及伊蚊，使加勒比海和巴西的野生种群减少了80- 96%。目前技术的一个昂贵方面是只选择雄性进行释放的手段，这一点很重要，因为雌性吸血并传播疾病。该项目旨在开发一种新的方法来生产RIDL蚊子的雄性群体（“遗传性别鉴定”），这将显着降低成本并巩固Oxitec在该领域的领导地位。将分析下一代测序数据集，以帮助在Ae中开发新的RIDL性状。埃及蚊子，以提高我们在实地应用技术的效率。有针对性的生物信息学方法的发展，以及一种新的遗传性别鉴定技术的成功工程，将为在其他对公共卫生和农业重要的害虫中开发新的RIDL性状提供一个管道。

项目成果

The sequence of a male-specific genome region containing the sex determination switch in Aedes aegypti

埃及伊蚊中含有性别决定开关的雄性特异性基因组区域的序列

• DOI: 10.1101/122804
• 发表时间: 2017
• 作者: Turner J

Additional file 1: of The sequence of a male-specific genome region containing the sex determination switch in Aedes aegypti

附加文件 1：包含埃及伊蚊性别决定开关的雄性特异性基因组区域的序列

• DOI: 10.6084/m9.figshare.7233563
• 发表时间: 2018
• 作者: Turner J

The sequence of a male-specific genome region containing the sex determination switch in Aedes aegypti.

• DOI: 10.1186/s13071-018-3090-3
• 发表时间: 2018-10-20
• 期刊: Parasites & vectors
• 影响因子: 3.2
• 作者: Turner J;Krishna R;Van't Hof AE;Sutton ER;Matzen K;Darby AC
• 通讯作者: Darby AC

Recovering individual haplotypes and a contiguous genome assembly from pooled long-read sequencing of the diamondback moth (Lepidoptera: Plutellidae).

• DOI: 10.1093/g3journal/jkac210
• 发表时间: 2022-09-30
• 期刊: G3 (Bethesda, Md.)