High quality long read sequencing from low input DNA

点击翻译按钮获取中文摘要

Genomics is a fast-moving field that is constantly been driven forward by developments in sequencing technology. To stay at the leading edge of this field it is important that we invest periodically in the latest equipment. In this application the Centre for Genomic Research (CGR) at the University of Liverpool proposes to purchase a Pacific Biosciences Sequel II and a FemtoPulse system to extend its current sequencing provision. These instruments will provide new, unique capability-- generation of long-read DNA sequence data from limited amounts of DNA, enabling new applications in de novo genome assembly, microbiome analysis, AMR and gene regulation. The CGR has a long-track record of successfully running PacBio long-read sequencing platforms and of supporting the research community by providing access to genomic technologies. The Sequel II will be placed in an established laboratory, with 20 staff and ~£5m of equipment. Our experience with PacBio platforms specifically means that the Sequel II can be deployed immediately on arrival, replacing our Sequel I platform, which will soon be uneconomical, unable to meet researchers' expectations, and hence functionally becoming obsolete.