ERA-CAPS 13 Delineating the crossover control networks in plants (DeCOP)

ERA-CAPS 13 描绘工厂中的交叉控制网络 (DeCOP)

• 批准号: BB/M004902/1
• 负责人: Chris Franklin
• 金额: $ 53.84万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM004902%2F1
• 关键词: ERA; CAPS; 13; Delineating; crossover

Meiosis is a specialized type of cell division required for sexual reproduction. It ensures the reduction of the genome and the recombination of maternal and paternal chromosomal segments prior to the formation of generative cells. Understanding the factors that control the initiation of recombination and processing recombination intermediates to form crossovers is of fundamental scientific interest, moreover this knowledge will have important implications for manipulating meiotic recombination in crop plants. In recent years meiosis research in plants has largely focussed on the identification of meiotic genes/proteins involved in recombination pathways or the organization of the chromosome axes and the synaptonemal complex, a specialized proteinaceous structure transiently formed during meiosis between the pairs of homologous chromosomes. Although these studies clearly demonstrate the importance of these proteins, it remains largely enigmatic how their activities are coordinated to ensure the controlled formation of crossovers. Key questions relate to how recombination is controlled such that each pair of homologous chromosomes receives at least one genetic crossover and how any additional crossovers are prevented from occurring in an adjacent chromosomal region; a phenomenon termed crossover interference.This collaborative project seeks to shift emphasis to focus on how recombination, chromosome organisation and remodelling are orchestrated to control the frequency and distribution of crossover events. Specifically, we seek to identify the protein networks that determine the fate of the individual DNA double-strand breaks that initiate recombination and establish when CO interference is established. We aim to identify novel factors that modulate crossover formation and interference; to investigate the role of chromosome axis-associated proteins in CO maturation and interference, to determine the role of protein phosphorylation in coordinating meiotic DNA repair and crossover formation; to identify proteins involved in the final step of crossover formation. We anticipate the factors and processes studied in the project will significantly enhance our understanding of the networks that govern crossover formation in plants. We therefore believe that our findings will strongly stimulate future crop breeding programmes.

减数分裂是有性生殖所需的一种特殊类型的细胞分裂。它确保在生殖细胞形成之前基因组的减少以及母本和父本染色体片段的重组。了解控制重组起始和处理重组中间体以形成交叉的因素具有基本的科学意义，此外，这些知识将对操纵作物减数分裂重组具有重要意义。近年来，植物减数分裂研究主要集中于鉴定参与重组途径或染色体轴组织和联会复合体的减数分裂基因/蛋白质，联会复合体是减数分裂过程中同源染色体对之间瞬时形成的特殊蛋白质结构。尽管这些研究清楚地证明了这些蛋白质的重要​​性，但如何协调它们的活性以确保交叉的受控形成仍然很大程度上是个谜。关键问题涉及如何控制重组，使每对同源染色体至少接受一次遗传交换，以及如何防止相邻染色体区域发生任何额外的交换；一种称为交叉干扰的现象。这个合作项目试图将重点转移到如何协调重组、染色体组织和重塑来控制交叉事件的频率和分布。具体来说，我们试图识别决定单个 DNA 双链断裂命运的蛋白质网络，这些断裂启动重组并在 CO 干扰建立时建立。我们的目标是找出调节交叉形成和干扰的新因素；研究染色体轴相关蛋白在 CO 成熟和干扰中的作用，确定蛋白磷酸化在协调减数分裂 DNA 修复和交换形成中的作用；鉴定参与交叉形成最后一步的蛋白质。我们预计该项目中研究的因素和过程将显着增强我们对植物中控制交叉形成的网络的理解。因此，我们相信我们的发现将强烈刺激未来的作物育种计划。

项目成果

Meiotic chromosome axis remodelling is critical for meiotic recombination in Brassica rapa.

• DOI: 10.1093/jxb/erab035
• 发表时间: 2021-04-02
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Cuacos M;Lambing C;Pachon-Penalba M;Osman K;Armstrong SJ;Henderson IR;Sanchez-Moran E;Franklin FCH;Heckmann S
• 通讯作者: Heckmann S

Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers

拟南芥 PCH2 介导减数分裂染色体重塑和交叉成熟

• DOI: 10.17615/pp5r-xg77
• 发表时间: 2015
• 作者: Armstrong, Susan J.

The challenge of evolving stable polyploidy: could an increase in "crossover interference distance" play a central role?

• DOI: 10.1007/s00412-015-0571-4
• 发表时间: 2016-06
• 期刊: Chromosoma
• 影响因子: 1.6
• 作者: Bomblies K;Jones G;Franklin C;Zickler D;Kleckner N
• 通讯作者: Kleckner N

Atypical centromeres in plants-what they can tell us.

• DOI: 10.3389/fpls.2015.00913
• 发表时间: 2015
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Cuacos M;H Franklin FC;Heckmann S
• 通讯作者: Heckmann S

Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers.

• DOI: 10.1371/journal.pgen.1005372
• 发表时间: 2015-07
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Lambing C;Osman K;Nuntasoontorn K;West A;Higgins JD;Copenhaver GP;Yang J;Armstrong SJ;Mechtler K;Roitinger E;Franklin FC
• 通讯作者: Franklin FC