ERA-CAPS 13 Delineating the crossover control networks in plants (DeCOP)

ERA-CAPS 13 描绘工厂中的交叉控制网络 (DeCOP)

基本信息

批准号：
BB/M004902/1
负责人：
Chris Franklin
金额：
$ 53.84万
依托单位：
University of Birmingham
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2014
资助国家：
英国
起止时间：
2014 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FM004902%2F1
关键词：
ERA CAPS 13 Delineating crossover

项目摘要

Meiosis is a specialized type of cell division required for sexual reproduction. It ensures the reduction of the genome and the recombination of maternal and paternal chromosomal segments prior to the formation of generative cells. Understanding the factors that control the initiation of recombination and processing recombination intermediates to form crossovers is of fundamental scientific interest, moreover this knowledge will have important implications for manipulating meiotic recombination in crop plants. In recent years meiosis research in plants has largely focussed on the identification of meiotic genes/proteins involved in recombination pathways or the organization of the chromosome axes and the synaptonemal complex, a specialized proteinaceous structure transiently formed during meiosis between the pairs of homologous chromosomes. Although these studies clearly demonstrate the importance of these proteins, it remains largely enigmatic how their activities are coordinated to ensure the controlled formation of crossovers. Key questions relate to how recombination is controlled such that each pair of homologous chromosomes receives at least one genetic crossover and how any additional crossovers are prevented from occurring in an adjacent chromosomal region; a phenomenon termed crossover interference.This collaborative project seeks to shift emphasis to focus on how recombination, chromosome organisation and remodelling are orchestrated to control the frequency and distribution of crossover events. Specifically, we seek to identify the protein networks that determine the fate of the individual DNA double-strand breaks that initiate recombination and establish when CO interference is established. We aim to identify novel factors that modulate crossover formation and interference; to investigate the role of chromosome axis-associated proteins in CO maturation and interference, to determine the role of protein phosphorylation in coordinating meiotic DNA repair and crossover formation; to identify proteins involved in the final step of crossover formation. We anticipate the factors and processes studied in the project will significantly enhance our understanding of the networks that govern crossover formation in plants. We therefore believe that our findings will strongly stimulate future crop breeding programmes.

减数分裂是有性繁殖所需的一种专门类型的细胞分裂类型。它确保在形成生成细胞之前的基因组还原和母体和父亲染色体片段的重组。了解控制重组和加工重组中间体以形成跨界的因素具有基本的科学意义，此外，这些知识将对处理农作物植物的减数分裂重组具有重要意义。近年来，植物中的减数分裂研究主要集中在鉴定参与重组途径的减数分裂基因/蛋白质，或组织染色体轴的组织和突发型复合物，这是一种特殊的蛋白质结构，这是一种在同源性染色体对之间的减数分裂过程中在减学过程中暂时形成的。尽管这些研究清楚地表明了这些蛋白质的重要性，但在很大程度上仍然是神秘的活动方式，以确保交叉的受控形成。关键问题与如何控制重组有关，使每对同源染色体至少接收一个遗传跨界，以及如何阻止在相邻的染色体区域中发生任何其他交叉。这种合作项目试图将重点放在重组，重组，染色体组织和重塑如何精心策划以控制交叉事件的频率和分布方面，将重点放在重点上。具体而言，我们试图确定确定启动重组并确定CO干扰的单个DNA双链断裂的命运的蛋白质网络。我们旨在确定调节跨界形成和干扰的新因素。为了研究染色体轴相关蛋白在成熟和干扰中的作用，以确定蛋白质磷酸化在协调减数分裂DNA修复和交叉形成中的作用；确定涉及交叉形成最后一步的蛋白质。我们预计项目中研究的因素和过程将显着增强我们对控制植物交叉形成的网络的理解。因此，我们认为我们的发现将强烈刺激未来的作物育种计划。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Meiotic chromosome axis remodelling is critical for meiotic recombination in Brassica rapa.

DOI：
10.1093/jxb/erab035
发表时间：
2021-04-02
期刊：
Journal of experimental botany
影响因子：
6.9
作者：
Cuacos M;Lambing C;Pachon-Penalba M;Osman K;Armstrong SJ;Henderson IR;Sanchez-Moran E;Franklin FCH;Heckmann S
通讯作者：
Heckmann S

Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers

拟南芥 PCH2 介导减数分裂染色体重塑和交叉成熟

DOI：
10.17615/pp5r-xg77
发表时间：
2015
期刊：
影响因子：
0
作者：
Armstrong, Susan J.
通讯作者：
Armstrong, Susan J.

The challenge of evolving stable polyploidy: could an increase in "crossover interference distance" play a central role?

DOI：
10.1007/s00412-015-0571-4
发表时间：
2016-06
期刊：
Chromosoma
影响因子：
1.6
作者：
Bomblies K;Jones G;Franklin C;Zickler D;Kleckner N
通讯作者：
Kleckner N

Atypical centromeres in plants-what they can tell us.

DOI：
10.3389/fpls.2015.00913
发表时间：
2015
期刊：
Frontiers in plant science
影响因子：
5.6
作者：
Cuacos M;H Franklin FC;Heckmann S
通讯作者：
Heckmann S

Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers.

DOI：
10.1371/journal.pgen.1005372
发表时间：
2015-07
期刊：
PLoS genetics
影响因子：
4.5
作者：
Lambing C;Osman K;Nuntasoontorn K;West A;Higgins JD;Copenhaver GP;Yang J;Armstrong SJ;Mechtler K;Roitinger E;Franklin FC
通讯作者：
Franklin FC