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13 ERA-CAPS FLOWPLAST

基本信息

批准号：
BB/M000338/1
负责人：
Brendan Davies
金额：
$ 57.57万
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2014
资助国家：
英国
起止时间：
2014 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FM000338%2F1
关键词：
13 ERA CAPS FLOWPLAST

项目摘要

Flowering is precisely controlled by diverse environmental cues. These responses contribute to the adaptation of plants to different environments and to the optimisation of crop yields. Genetic and molecular analyses performed mainly in Arabidopsis thaliana have identified regulatory pathways that confer different responses to the environment and integrate endogenous and environmental cues to control the floral transition. These pathways act in different tissues of the plant but ultimately influence flowering at the shoot meristem. A complete understanding of these pathways and how they are integrated will decipher how plasticity in flowering response is conferred among plants of the same genotype and how genetic variation between varieties impacts on this plasticity. In turn such knowledge will increase our capacity to breed crops for changing environmental conditions. We propose to investigate the molecular basis of plasticity in flowering-time control in A. thaliana, with a focus on two crucial environmental cues, increases in ambient temperature and different day lengths. As the pathways mediating these responses ultimately converge on the shoot meristem to initiate transcriptional reprogramming we will develop the INTACT system to describe the transcriptional changes that occur in defined spatial zones of the meristem through a temporal series in response to changes in day length. Then, we will use the data from the INTACT profiling to address specific issues related to signal integration at the meristem. We will investigate the chromatin landscape at the shoot apical meristem (SAM) during the transition to flowering and correlate this to transcriptional activity and splicing patterns in response to high ambient temperatures. Such analyses will allow comparison of these data with those obtained during the day-length series identifying common and distinct processes. In addition, we will investigate the integration of different signalling pathways in the rib meristem, where photoperiod and gibberellic acid (GA) signalling converge. Thus, this project will employ flowering and advances in genomic technologies as a platform for dissecting basic mechanisms that govern developmental plasticity on a whole-genome scale. Our results will be of interest to a wide audience, and will help to answer the evolutionarily important questions of how environmental signalling pathways are prioritized and integrated at the shoot meristem as well as how much epigenetic regulation and alternative splicing contributes to the floral transition. Ultimately such knowledge will help predict the mechanisms available to plants to manipulate the timing of the floral transition in response to environmental changes and will contribute to sustainable agriculture.

开花是由各种环境因素精确控制的。这些反应有助于植物适应不同的环境和优化作物产量。主要在拟南芥中进行的遗传和分子分析已经确定了对环境产生不同反应的调控途径，并整合了内源和环境线索来控制花的转变。这些途径作用于植物的不同组织，但最终影响茎分生组织的开花。对这些通路的完整理解以及它们是如何整合的，将有助于解释相同基因型植物开花反应的可塑性是如何被赋予的，以及品种间的遗传变异是如何影响这种可塑性的。反过来，这些知识将提高我们培育适应不断变化的环境条件的作物的能力。研究拟南芥（a.thaliana）开花时间调控可塑性的分子基础，重点关注两个关键的环境因素，即环境温度的升高和不同的日照长度。由于介导这些反应的途径最终集中在茎分生组织上，从而启动转录重编程，我们将开发一个完整的系统，通过时间序列来描述分生组织中特定空间区域发生的转录变化，以响应白昼长度的变化。然后，我们将使用来自完整分析的数据来解决与分生系统信号集成相关的具体问题。我们将研究开花过渡期间茎尖分生组织（SAM）的染色质景观，并将其与高温下的转录活性和剪接模式联系起来。这种分析将允许将这些数据与在确定共同和独特过程的日间序列中获得的数据进行比较。此外，我们将研究肋骨分生组织中不同信号通路的整合，其中光周期和赤霉素（GA）信号汇聚。因此，该项目将利用开花和基因组技术的进步作为一个平台，在全基因组规模上剖析控制发育可塑性的基本机制。我们的研究结果将引起广泛的兴趣，并将有助于回答环境信号通路如何在茎分生组织中被优先考虑和整合，以及表观遗传调控和选择性剪接在多大程度上促进了花的转变等进化上的重要问题。最终，这些知识将有助于预测植物在应对环境变化时操纵花卉转变时间的可用机制，并将有助于可持续农业。