13 ERA-CAPS FLOWPLAST

基本信息

批准号：
BB/M000338/1
负责人：
Brendan Davies
金额：
$ 57.57万
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2014
资助国家：
英国
起止时间：
2014 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FM000338%2F1
关键词：
13 ERA CAPS FLOWPLAST

项目摘要

Flowering is precisely controlled by diverse environmental cues. These responses contribute to the adaptation of plants to different environments and to the optimisation of crop yields. Genetic and molecular analyses performed mainly in Arabidopsis thaliana have identified regulatory pathways that confer different responses to the environment and integrate endogenous and environmental cues to control the floral transition. These pathways act in different tissues of the plant but ultimately influence flowering at the shoot meristem. A complete understanding of these pathways and how they are integrated will decipher how plasticity in flowering response is conferred among plants of the same genotype and how genetic variation between varieties impacts on this plasticity. In turn such knowledge will increase our capacity to breed crops for changing environmental conditions. We propose to investigate the molecular basis of plasticity in flowering-time control in A. thaliana, with a focus on two crucial environmental cues, increases in ambient temperature and different day lengths. As the pathways mediating these responses ultimately converge on the shoot meristem to initiate transcriptional reprogramming we will develop the INTACT system to describe the transcriptional changes that occur in defined spatial zones of the meristem through a temporal series in response to changes in day length. Then, we will use the data from the INTACT profiling to address specific issues related to signal integration at the meristem. We will investigate the chromatin landscape at the shoot apical meristem (SAM) during the transition to flowering and correlate this to transcriptional activity and splicing patterns in response to high ambient temperatures. Such analyses will allow comparison of these data with those obtained during the day-length series identifying common and distinct processes. In addition, we will investigate the integration of different signalling pathways in the rib meristem, where photoperiod and gibberellic acid (GA) signalling converge. Thus, this project will employ flowering and advances in genomic technologies as a platform for dissecting basic mechanisms that govern developmental plasticity on a whole-genome scale. Our results will be of interest to a wide audience, and will help to answer the evolutionarily important questions of how environmental signalling pathways are prioritized and integrated at the shoot meristem as well as how much epigenetic regulation and alternative splicing contributes to the floral transition. Ultimately such knowledge will help predict the mechanisms available to plants to manipulate the timing of the floral transition in response to environmental changes and will contribute to sustainable agriculture.

开花精确控制着各种环境线索。这些反应有助于植物适应不同的环境和作物产量的优化。主要在拟南芥中进行的遗传和分子分析已经确定了对环境的不同反应并整合内源性和环境提示以控制花卉过渡的调节途径。这些途径在植物的不同组织中起作用，但最终会影响芽分生组织的开花。对这些途径的完整理解以及它们的整合方式将破译在相同基因型植物之间如何赋予开花反应中的可塑性，以及品种之间的遗传变异如何影响这种可塑性。反过来，这种知识将增加我们繁殖农作物以改变环境条件的能力。我们建议研究塔利亚纳（A. thaliana）开花时间对照中可塑性的分子基础，重点是两个关键的环境提示，升高环境温度和不同的白天长度。随着介导这些响应的途径最终会在芽分生组织上汇聚以启动转录重编程，我们将开发完整的系统，以通过时间序列来描述分生组织中定义的空间区域中发生的转录变化，以响应日长度的变化而响应。然后，我们将使用完整分析中的数据来解决分生组织处与信号集成有关的特定问题。我们将在向开花过渡期间研究芽顶分生组织（SAM）处的染色质景观，并将其与转录活性和剪接模式相关联，以响应高环境温度。这样的分析将允许将这些数据与在日长度序列中获得的数据进行比较，从而识别常见和不同的过程。此外，我们将研究在肋骨分生组织中不同信号通路的整合，其中光周期和gaberellic Acid（GA）信号收敛。因此，该项目将采用开花和基因组技术的进步，作为剖析基本机制以全基因组规模控制发展可塑性的平台。我们的结果将引起广泛的受众群体，并将有助于回答有关环境信号通路如何优先和整合在拍摄分生组织中以及有多少表观遗传调节和替代剪接有助于花卉过渡的进化重要问题。最终，这种知识将有助于预测植物可根据环境变化来操纵花卉过渡时机的机制，并有助于可持续农业。