13 ERA-CAPS The role of the N-end rule pathway in controlling plant response to the environment

13 ERA-CAPS N端规则途径在控制植物对环境反应中的作用

• 批准号: BB/M002268/1
• 负责人: Michael Holdsworth
• 金额: $ 54.89万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM002268%2F1
• 关键词: 13; ERA; CAPS; role; end

Manipulation of plants to provide stability of yield under unpredictable growth conditions will beessential to respond to the effect of climate change in increasing the uncertainty of the agriculturalenvironment. Selective and conditional removal of regulator proteins by proteolysis is emerging as amajor regulatory principle in plants. The aim of the N-vironment project is to provide a completemechanistic understanding of the role of the N-end rule pathway of targeted proteolysis incontrolling plant responses to the environment. To achieve this goal consortium partners have beenselected that represent internationally leading European teams with experience in the pathwaysmultiple facets, including the biochemical basis of the pathway, hypoxia and plant development,transcriptional regulation of stress and metabolic signalling. The project will bring together sixresearch groups with complementary expertise in fundamental molecular plant science,biochemistry and chemistry, in four institutions. The research programme of the N-vironmentconsortium will be achieved through six inter-related Work Packages carried out by the fourconsortium partners (three funded by ERA-CAPS, one associated laboratory). The objectives of thesework packages are related to three fundamental questions that arose after integration of the recentdiscoveries by consortium members of the role of the N-end rule pathway of targeted proteolysis as amajor regulator of plant development and response to the environment. Question 1: What are theprotein substrates and enzymatic components of the N-end rule pathway? Question 2: How is the Nendrule pathway integrated into cellular signaling pathways? Question 3: What is the extent of therole of the N-end rule pathway in plant response to the biotic and abiotic environment? The proposedresearch has highly innovative measurable outcomes that address this newly discovered area of plantbiology, and will uncover: New mechanisms regulating protein stability, new mechanisms ofenvironmental stress sensing, new functions for proteins in stress sensing, the importance of theN-end rule in a key EU crop, tomato. This fast-developing area of plant molecular science is led byN-vironment members (including discovery of substrates, methods of entry into the N-end rulepathway, biochemical components of the pathway), and together with the availability of a largenumber of unique resources within the consortium, makes the N-vironment proposal highly novel andtimely. The synergistic value of this collaborative programme will be the development andexploitation of a highly novel area of plant biology of key importance to agriculture, in which Europehas the capacity to take a world lead through ERA-CAPS funding.

操纵植物以在不可预测的生长条件下提供产量的稳定性，以应对气候变化对增加农业环境不确定性的影响。通过蛋白水解对调节蛋白的选择性去除和有条件去除是在植物中作为Amajor调节原理的出现。 N-Vorirnment项目的目的是对靶向蛋白水解对环境的反应的N端规则途径的作用提供完整的机理理解。为了实现这一目标联盟伙伴，已选择代表具有途径经验的国际领先的欧洲团队，包括途径，缺氧和植物发展的生化基础，压力和代谢信号的转录调节。该项目将在四个机构的基本分子植物科学，生物化学和化学方面汇集具有互补专业知识的SixResearch小组。 N-VironmentContortium的研究计划将通过FourConstortium Partners进行的六个相关工作包（由ERA-CAPS资助，一个是相关的实验室）来实现的。这些工作包的目标与财团成员在近期发现的N-End规则途径的作用后，作为植物开发和对环境的反应的Amajor调节剂的作用，这是与三个基本问题有关的三个基本问题。问题1：n端规则途径的蛋白质底物和酶促成分是什么？问题2：Nendrule途径如何整合到细胞信号通路中？问题3：植物对生物和非生物环境的植物反应中N端规则途径的质量范围是多少？拟议的研究具有高度创新的可测量结果，可以解决这一新发现的植物生物学领域，并将发现：调节蛋白质稳定性的新机制，环境应激感应的新机制，蛋白质在压力感应中的新功能，当时的EU eu作物中当时的末端规则的重要性，tomato。植物分子科学的这个快速发展领域是由N-Von-Virnment成员领导的（包括底物的发现，进入N-End Rulepathway的方法，途径的生化组成部分），以及在该协会中的可用性，以及在n-Vornigumber中的独特资源，使N-环境提案的高度新颖新颖的新颖新颖。该协作计划的协同价值将是开发和开发对农业关键重要性的植物生物学领域的发展和探索，在该领域中，欧洲人有能力通过ERA-CAPS资金来实现世界。

项目成果

A Yeast-Based Functional Assay to Study Plant N-Degron - N-Recognin Interactions.

• DOI: 10.3389/fpls.2021.806129
• 发表时间: 2021
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Kozlic A;Winter N;Telser T;Reimann J;Rose K;Nehlin L;Berckhan S;Sharma G;Dambire C;Boeckx T;Holdsworth MJ;Bachmair A
• 通讯作者: Bachmair A