13 ERA-CAPS The role of the N-end rule pathway in controlling plant response to the environment

13 ERA-CAPS N端规则途径在控制植物对环境反应中的作用

• 批准号: BB/M002268/1
• 负责人: Michael Holdsworth
• 金额: $ 54.89万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM002268%2F1
• 关键词: 13; ERA; CAPS; role; end

Manipulation of plants to provide stability of yield under unpredictable growth conditions will beessential to respond to the effect of climate change in increasing the uncertainty of the agriculturalenvironment. Selective and conditional removal of regulator proteins by proteolysis is emerging as amajor regulatory principle in plants. The aim of the N-vironment project is to provide a completemechanistic understanding of the role of the N-end rule pathway of targeted proteolysis incontrolling plant responses to the environment. To achieve this goal consortium partners have beenselected that represent internationally leading European teams with experience in the pathwaysmultiple facets, including the biochemical basis of the pathway, hypoxia and plant development,transcriptional regulation of stress and metabolic signalling. The project will bring together sixresearch groups with complementary expertise in fundamental molecular plant science,biochemistry and chemistry, in four institutions. The research programme of the N-vironmentconsortium will be achieved through six inter-related Work Packages carried out by the fourconsortium partners (three funded by ERA-CAPS, one associated laboratory). The objectives of thesework packages are related to three fundamental questions that arose after integration of the recentdiscoveries by consortium members of the role of the N-end rule pathway of targeted proteolysis as amajor regulator of plant development and response to the environment. Question 1: What are theprotein substrates and enzymatic components of the N-end rule pathway? Question 2: How is the Nendrule pathway integrated into cellular signaling pathways? Question 3: What is the extent of therole of the N-end rule pathway in plant response to the biotic and abiotic environment? The proposedresearch has highly innovative measurable outcomes that address this newly discovered area of plantbiology, and will uncover: New mechanisms regulating protein stability, new mechanisms ofenvironmental stress sensing, new functions for proteins in stress sensing, the importance of theN-end rule in a key EU crop, tomato. This fast-developing area of plant molecular science is led byN-vironment members (including discovery of substrates, methods of entry into the N-end rulepathway, biochemical components of the pathway), and together with the availability of a largenumber of unique resources within the consortium, makes the N-vironment proposal highly novel andtimely. The synergistic value of this collaborative programme will be the development andexploitation of a highly novel area of plant biology of key importance to agriculture, in which Europehas the capacity to take a world lead through ERA-CAPS funding.

在不可预测的生长条件下，操纵植物以提供稳定的产量对于应对气候变化增加农业环境不确定性的影响至关重要。通过蛋白质水解选择性地和有条件地去除调节蛋白是植物中一个重要的调节原理。N末端酶解项目的目的是提供一个完整的机制理解的作用，N末端规则途径的目标蛋白质水解在控制植物对环境的反应。为了实现这一目标，选择了代表国际领先的欧洲团队的联盟合作伙伴，这些团队在途径多个方面都有经验，包括途径的生化基础，缺氧和植物发育，应激的转录调节和代谢信号。该项目将汇集四个机构的六个研究小组，这些研究小组在基础分子植物科学、生物化学和化学方面具有互补的专门知识。N-CAPS联合体的研究方案将通过四个联合体伙伴（三个由ERA-CAPS资助，一个是相关实验室）开展的六个相互关联的工作包来实现。这些工作包的目标涉及到三个基本问题，这些问题是在整合了联盟成员最近的发现之后产生的，这些发现是关于N端规则途径作为植物发育和对环境响应的主要调节剂的作用。问题1：N端规则途径的蛋白质底物和酶组分是什么？问题2：Nendrule通路如何整合到细胞信号通路中？问题3：N端规则途径在植物对生物和非生物环境的反应中发挥了多大的作用？该研究具有高度创新性的可测量结果，解决了植物生物学的这一新发现的领域，并将揭示：调节蛋白质稳定性的新机制，环境胁迫传感的新机制，蛋白质在胁迫传感中的新功能，N端规则在欧盟关键作物番茄中的重要性。这一快速发展的植物分子科学领域是由N-Envelopment成员领导的（包括底物的发现、进入N-末端规则途径的方法、途径的生化成分），加上该联盟内大量独特资源的可用性，使得N-Envelopment的提议非常新颖和及时。这一合作计划的协同价值将是开发和利用一个对农业至关重要的高度新颖的植物生物学领域，欧洲有能力通过ERA-CAPS资助在该领域发挥世界领先作用。

项目成果

A Yeast-Based Functional Assay to Study Plant N-Degron - N-Recognin Interactions.

• DOI: 10.3389/fpls.2021.806129
• 发表时间: 2021
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Kozlic A;Winter N;Telser T;Reimann J;Rose K;Nehlin L;Berckhan S;Sharma G;Dambire C;Boeckx T;Holdsworth MJ;Bachmair A
• 通讯作者: Bachmair A