14TSB_ESAP Improved risk prediction for precision agriculture: automated monitoring of pathogen movement

14TSB_ESAP 改进精准农业的风险预测：自动监测病原体运动

• 批准号: BB/M005453/1
• 负责人: Daniel McCluskey
• 金额: $ 31.63万
• 依托单位: University of Hertfordshire
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM005453%2F1
• 关键词: 14TSB_ESAP; Improved; risk; prediction; precision

The project seeks to develop an innovative automated in-field instrument for integrated spore-sampling and testing for airborne fungal pathogens in wheat which currently cost the UK in excess of £120 million annually to control. The instrument will form the basis of a distributed detection network, providing real-time information on inoculum movement, allowing more effective timing and targeting of fungicide control. This will aid the crop science industry to further research risk prediction and development/refinement of decision support systems for fungal pathogens, benefitting the cereal industry in the first instance, but also the UK society in general by helping to secure the long term future of the food industry. The work involves integration of cyclone air sampling, automated fluidic handling and DNA analysis using Loop mediated isothermal AMPlification (LAMP) methods for direct detection and identification of fungal species. The University of Hertfordshire's (UoH) primary involvement, and thus our core objectives, are the development of the particulate capture and transport mechanisms within this unit.In particular the primary objective is the development of a cyclonic separator for spore collection (Septoria tritici, Puccinia triticina and P striiformis) with subsequent fluid handling and sample processing platform development. The cyclone separator forms the initial stage of a collection unit which will also include a particle retention chamber and mechanical/fluidic interface. The latter, comprising both milli and microfluidic systems, transfers collected analyte to the processing module to prepare the sample for the detection chamber, wherein detection will be based on sensitive real-time fluorescence detection of the target DNA. An engineering solution will be developed to integrate the air sampling, fluid handling and detection platforms.The system will sample periodically with the collected analyte analysed in batches. The approach that we propose here will offer rapid and accurate analysis of samples collected by remotely distributed in-field instrumentation. In order to achieve our objectives the work plan for UoH, is as follows:Objective 1: Development of in-field cyclonic fungal spore collector (Lead UoH)Milestone (MS) 1.1 Design & development of cyclonic collector and intake assemblyMS 1.2 Design & development of fluidic handling and processing moduleMS 1.3 Manufacture & evaluation of prototype cyclonic collector and intake assemblyMS 1.4 Cyclone aerosol collection characterisation and performance tests with dry spore simulantsMS 1.5 Manufacture & evaluation of prototype fluidic handling and processing moduleMS 1.6 Manufacture & evaluation of fluidic handling and processing moduleObjective 2: Evaluation of sample processing of fungal spores (Lead Fera)MS 2.2 Integration of sample preparation procedure with fluidic handling and processing moduleThe aim is to contribute to the core development of a precision agriculture tool to enhance efficient use of resources in the crop, maximise yield and minimise potentially negative environmental impacts associated with food production in line with the scope of the competition. The proposal brings together the knowledge and skills of a cross-disciplinary team from designers and engineers of scientific instruments (Optisense and University of Hertfordshire), representatives of the crop protection industry (Bayer), and plant pathology/detection experts (Fera). The resultant tool will therefore have input from a broad spectrum of crop science stakeholders.However, developing these new technologies is only part of the challenge. It is also necessary to make sure these new methods are fit-for-purpose and that they work in a way that meets the needs of end users including research scientists, food producers and agri-chemical specialists. To achieve this, core end users are represented in the development work within this consortium.

该项目旨在开发一种创新的自动化现场仪器，用于集成孢子采样和测试小麦中的空气传播真菌病原体，目前英国每年的控制成本超过1.2亿英镑。该仪器将构成分布式检测网络的基础，提供关于接种物移动的实时信息，从而能够更有效地控制杀真菌剂的时间和目标。这将有助于作物科学行业进一步研究真菌病原体的风险预测和决策支持系统的开发/完善，首先使谷物行业受益，但也有助于确保食品行业的长期未来，从而使英国社会普遍受益。该工作涉及旋风空气采样、自动流体处理和DNA分析的集成，使用环介导的等温AMP液化（LAMP）方法直接检测和鉴定真菌物种。赫特福德大学（UoH）的主要参与，因此我们的核心目标，是在这个单位的颗粒捕获和运输机制的发展，特别是主要目标是一个旋风分离器的孢子收集（壳针孢，小麦柄锈菌和P striiformis）的发展，随后的流体处理和样品处理平台的发展。旋风分离器形成收集单元的初始级，该收集单元还将包括颗粒保留室和机械/流体接口。后者包括毫微系统和微流体系统，将收集的分析物转移到处理模块以制备用于检测室的样品，其中检测将基于靶DNA的灵敏实时荧光检测。将开发一个工程解决方案，以整合空气采样、流体处理和检测平台。该系统将定期采样，并分批分析收集的分析物。我们在这里提出的方法将提供快速和准确的分析远程分布式现场仪器收集的样品。为了实现我们的目标，卫生组织的工作计划如下：田间旋风式真菌孢子收集器的研制（领导UoH）里程碑（MS）1.1旋风收集器和进气组件的设计和开发MS 1.2流体处理和处理模块的设计和开发MS 1.3制造和生产原型旋风收集器和进气组件的评价MS 1.4旋风气溶胶收集特性和干孢子模拟物的性能试验MS 1.5制造和原型流体处理和处理模块的评估MS1.6流体处理和处理模块的制造和评估目标2：真菌孢子样品处理的评价（Lead Fera）MS 2.2样品制备程序与流体处理和处理模块的集成目的是为精准农业工具的核心开发做出贡献提高作物资源的有效利用，最大限度地提高产量，并根据竞争范围将与粮食生产相关的潜在负面环境影响降至最低。该提案汇集了来自科学仪器设计师和工程师（Optisense和赫特福德大学），作物保护行业代表（拜耳）和植物病理学/检测专家（Fera）的跨学科团队的知识和技能。因此，由此产生的工具将得到广泛的作物科学利益相关者的投入。然而，开发这些新技术只是挑战的一部分。还必须确保这些新方法适用于目的，并以满足最终用户（包括研究科学家，食品生产商和农业化学专家）需求的方式工作。为了实现这一目标，核心最终用户代表参与了该联盟的开发工作。

项目成果

Design of a high efficiency cyclone for collection of rare and low concentration airborne pathogens

一种用于收集稀有低浓度空气传播病原体的高效旋风分离器的设计

• 发表时间: 2017
• 作者: Baxter R