[16-FAPESP-BE] Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals

[16-FAPESP-BE] 纤维素乙醇工厂中的木质素增值：通过阿魏酸生物催化转化为高价值化学品

• 批准号: BB/P01738X/1
• 负责人: Timothy Bugg
• 金额: $ 265.34万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FP01738X%2F1
• 关键词: 16; FAPESP; Lignin; valorization; cellulosic

Lignin is a polymer found in plant cell walls, and is the most abundant source of renewable aromatic material on Earth. Lignin therefore represents a valuable raw material for generation of renewable chemicals, which will help society to reduce its dependance on crude oil for production of chemicals and materials such as plastics. Converting lignin into renewable chemicals is a very difficult challenge, because it is very hard to break down, and it is very heterogeneous (mixture of different structural units). Researchers at Warwick University have recently discovered several soil bacteria that can break down lignin, and specific enzyme biocatalysts that can oxidise lignin, and through a BBSRC/FAPESP FAPPA award have collaborated with CTBE in Brazil to identify new lignin-degrading enzymes through genome sequencing, and to develop new "biosensors" that could be used to engineer recombinant lignin-degrading micro-organisms that could break down lignin to high-value chemicals.The proposal brings together expertise in cellulosic ethanol production and metagenomic DNA sequencing (CTBE) with expertise in biocatalytic lignin valorisation (Warwick) and biocatalysis for high value chemicals production (Manchester, UCL). The overall aim is to use synthetic biology to break down lignin to intermediate ferulic acid, which has been generated from lignin via bacterial fermentation in previous work, and then to convert ferulic acid via biocatalysis into high-value chemicals. The project will : 1) optimise a lignin stream for the project from cellulosic bioethanol production at the CTBE pilot plant; 2) convert lignin into ferulic acid from lignin using synthetic biology; 3) enzymatically convert ferulic acid into a high-value pharmaceutical chemical, L-Dopa; 4) generate high value fragrance chemicals (coniferyl acetate, isoeugenol) from ferulic acid; 5) scale up chemicals production from renewable feedstocks; 6) assess the technical and sustainability impact of the methods developed in the project.

木质素是在植物细胞壁中发现的聚合物，是地球上最丰富的可再生芳族材料的来源。因此，木质素代表了生成可再生化学物质的宝贵原材料，这将有助于社会减少对原油生产化学物质和材料（例如塑料）的依赖。将木质素转化为可再生化学物质是一个非常困难的挑战，因为它很难分解，并且非常异质（不同结构单元的混合物）。 Researchers at Warwick University have recently discovered several soil bacteria that can break down lignin, and specific enzyme biocatalysts that can oxidise lignin, and through a BBSRC/FAPESP FAPPA award have collaborated with CTBE in Brazil to identify new lignin-degrading enzymes through genome sequencing, and to develop new "biosensors" that could be used to engineer recombinant可能将木质素分解为高价值化学物质的木质素降解的微生物。该提案汇集了纤维素乙醇生产和元基因组DNA测序（CTBE）的专业知识，并在生物催化木质素量化（Warwick）中具有专业知识，并为高价值化学物质生产（Manchesters）生产（WARWICK）和生物价值化学物质的生产（Manchester）。总体目的是利用合成生物学将木质素分解为中间阿魏酸，这是在先前的工作中通过细菌发酵从木质素产生的，然后通过生物催化转化为高价值化学物质的阿魏酸。该项目将：1）优化CTBE试验厂纤维素生物乙醇的项目的木质素流； 2）使用合成生物学将木质素从木质素中转化为阿魏酸； 3）酶促将阿魏酸转化为高价值药物l-dopa； 4）从阿魏酸产生高价值的香料化学物质（乙酸乙酸盐酯，isougenol）； 5）从可再生原料中扩大化学品的生产； 6）评估项目中开发的方法的技术和可持续性影响。

项目成果

Microbial hosts for metabolic engineering of lignin bioconversion to renewable chemicals

• DOI: 10.1016/j.rser.2021.111674
• 发表时间: 2021-10-06
• 期刊: RENEWABLE & SUSTAINABLE ENERGY REVIEWS
• 影响因子: 15.9
• 作者: Bugg, Timothy D. H.;Williamson, James J.;Alberti, Fabrizio
• 通讯作者: Alberti, Fabrizio

A biocatalytic cascade for the conversion of fatty acids to fatty amines

• DOI: 10.1039/c9gc02260k
• 发表时间: 2019-09-21
• 期刊: GREEN CHEMISTRY
• 影响因子: 9.8
• 作者: Citoler, Joan;Derrington, Sasha R.;Turner, Nicholas J.
• 通讯作者: Turner, Nicholas J.

Engineered co-culture for consolidated production of phenylpropanoids directly from aromatic-rich biomass.

• DOI: 10.1016/j.biortech.2023.129935
• 发表时间: 2023-11
• 期刊: Bioresource technology
• 影响因子: 11.4
• 作者: Micaela Chacόn;Ellen Percival;T. Bugg;N. Dixon

Elucidation of microbial lignin degradation pathways using synthetic isotope-labelled lignin.

• DOI: 10.1039/d2cb00173j
• 发表时间: 2023-01-04
• 期刊: RSC CHEMICAL BIOLOGY
• 影响因子: 4.1
• 作者: Alruwaili, Awatif;Rashid, Goran M. M.;Sodre, Victoria;Mason, James;Rehman, Zainab;Menakath, Anjali K.;Cheung, David;Brown, Steven P.;Bugg, Timothy D. H.
• 通讯作者: Bugg, Timothy D. H.