Enhanced productivity and functionality of Modified Ribosomally Produced Peptides (M-RIPPs)

增强修饰核糖体产生的肽 (M-RIPP) 的生产力和功能

• 批准号: BB/M028526/1
• 负责人: Marcel Jaspars
• 金额: $ 58.98万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM028526%2F1
• 关键词: Enhanced; productivity; functionality; Modified; Ribosomally

Ribosomally Produced Peptides (RIPPs) are widely recognised as one of the most promising classes of compounds with the potential to treat many diseases including infection, cancer & inflammation. They are of great interest to the pharma industry, but are extremely costly to produce/modify - even in milligram amounts. Through the utilisation of cutting-edge techniques in combinatorial synthetic biology, this project sets out to achieve a world first; namely, to produce bespoke libraries of Modified RIPPs (M-RIPPs) in vivo and in sufficient quantities to permit drug discovery screening. The project combines the fundamental knowledge of the natural processes involved in RIPP biosynthesis of the two premier UK academic groups active in the field with the applied expertise in industrial biosynthesis of a leading UK IB company. It will deliver a versatile yet robust technology platform for the production of M-RIPPs in vivo that will be transferred to a spinoff company to be formed around 18 months after project start.

核糖体产生的多肽(RIPP)被广泛认为是最有前途的化合物之一，具有治疗包括感染、癌症和炎症在内的许多疾病的潜力。制药业对它们非常感兴趣，但生产/修改它们的成本极其高昂--即使是毫克量。通过利用组合合成生物学中的尖端技术，该项目致力于实现世界第一；即在体内生产定制的修饰Ripps文库(M-Ripps)，并提供足够的数量来进行药物发现筛选。该项目结合了活跃在该领域的两个英国顶尖学术团体对RIPP生物合成所涉及的自然过程的基本知识，以及英国一家领先的IB公司在工业生物合成方面的应用专业知识。它将为在体内生产M-Ripps提供一个多功能但强大的技术平台，该平台将被转移到一个剥离公司，该公司将在项目开始约18个月后成立。

项目成果

N-Prenylation of Tryptophan by an Aromatic Prenyltransferase from the Cyanobactin Biosynthetic Pathway

• DOI: 10.1021/acs.biochem.8b00879
• 发表时间: 2018-12-18
• 期刊: BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Dalponte, Luca;Parajuli, Anirudra;Fewer, David P.
• 通讯作者: Fewer, David P.