21EBTA Engineering specialised metabolism and new cellular architectures in plants

21EBTA Engineering 植物中的专业代谢和新细胞结构

• 批准号: BB/W014173/1
• 负责人: Anne Osbourn
• 金额: $ 193.36万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW014173%2F1
• 关键词: 21EBTA; Engineering; specialised; metabolism; new

Plants have a rich endogenous metabolism that can be reprogrammed by genetic transformation. Transient expression techniques have allowed their development as photosynthetic chassis for low-cost high-yield production of vaccines, therapeutic proteins and metabolites. In recent years, several commercial large-scale facilities for transient plant-based bioproduction have been constructed, with products now reaching the market. However, it has proved difficult to engineer high yields of engineered products in true-breeding plant lines, which would pave the way for low-cost bioproduction and new generations of crop varieties.In this project, we will use fast transient expression systems to design and assemble an artificial synthetic pathway for the production of beta-amyrin, a precursor for many different types of triterpene. The triterpenoids are a large, chemically diverse group of natural products (over 20,000 reported to date), with a wide range of applications in the agricultural, food, cosmetic and pharmaceutical sectors. We will transfer this DNA-encoded pathway into the genome of a new model plant system, the liverwort Marchantia polymorpha. Marchantia is arguably the simplest and easiest land plant to work with at this time. We have constructed many new tools for work with Marchantia, and will use these to trigger beta-amyrin production to specialised oil body containing cells. Oil bodies are natural safe containers for accumulation of high concentrations of compounds in cells. We will then use known developmental regulators to engineer oil cell proliferation in Marchantia tissues to create high-yield harvestable plant organs. We believe the project will provide a prototype for whole organism engineering, and crop improvement.

植物具有丰富的内源代谢，可以通过遗传转化重新编程。瞬时表达技术已经允许它们发展为用于疫苗、治疗性蛋白质和代谢物的低成本高产量生产的光合底盘。近年来，已经建造了几个商业化的大规模设施，用于短暂的植物基生物生产，产品现已进入市场。然而，在真正育种的植物株系中设计高产量的工程产品已经被证明是困难的，这将为低成本生物生产和新一代作物品种铺平道路。在本项目中，我们将使用快速瞬时表达系统设计和组装一条人工合成途径，用于生产β-香树脂醇，这是许多不同类型三萜的前体。三萜类化合物是一个庞大的、化学上多样化的天然产物组（迄今为止报道的超过20，000种），在农业、食品、化妆品和制药部门具有广泛的应用。我们将把这种DNA编码的途径转移到一种新的模式植物系统地钱的基因组中。地钱可以说是最简单和最容易的土地植物工作在这个时候。我们已经构建了许多新的工具来处理地钱，并将使用这些工具来触发β-香树脂素生产到专门的油体细胞。油体是细胞中高浓度化合物积累的天然安全容器。然后，我们将使用已知的发育调节剂来工程化地钱组织中的油细胞增殖，以创造高产可收获的植物器官。我们相信该项目将为整个生物工程和作物改良提供原型。

项目成果

Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana.

• DOI: 10.1111/pbi.14048
• 发表时间: 2023-07
• 期刊: PLANT BIOTECHNOLOGY JOURNAL
• 影响因子: 13.8
• 作者: Kallam, Kalyani;Moreno-Gimenez, Elena;Mateos-Fernandez, Ruben;Tansley, Connor;Gianoglio, Silvia;Orzaez, Diego;Patron, Nicola
• 通讯作者: Patron, Nicola

Cas9-Mediated Targeted Mutagenesis in Plants.

• DOI: 10.1007/978-1-0716-1791-5_1
• 发表时间: 2022-01-01
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Dudley, Quentin M;Raitskin, Oleg;Patron, Nicola J
• 通讯作者: Patron, Nicola J

An improved Nicotiana benthamiana bioproduction chassis provides novel insights into nicotine biosynthesis

改进的本塞姆氏烟草生物生产底盘为尼古丁生物合成提供了新的见解

• DOI: 10.1101/2023.03.06.531326
• 发表时间: 2023
• 作者: Vollheyde K

New and Emerging Concepts in the Evolution and Function of Plant Biosynthetic Gene Clusters

• DOI: 10.1016/j.cogsc.2021.100568
• 发表时间: 2021-11
• 期刊: Current Opinion in Green and Sustainable Chemistry
• 影响因子: 9.3
• 作者: G. Polturak;Zhenhua Liu;A. Osbourn