13TSB_SynBio A synthetic biology-based approach to engineering triterpenoid saponins and optimisation for industrial applications

13TSB_SynBio 一种基于合成生物学的三萜皂苷工程方法和工业应用优化

• 批准号: BB/L004372/1
• 负责人: Anne Osbourn
• 金额: $ 24.3万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FL004372%2F1
• 关键词: 13TSB_SynBio; synthetic; biology; based; approach

Saponins have already found industrial application as foaming agents in the beverage, food and cosmetics industries. However, the breadth of triterpene saponin structures has not been fully explored or exploited, and their application limited by accessibility and availability from plant extracts, depite broad-ranging physical and bioactive properties documented in the literature. This project will demonstrate the optimisation of triterpene saponin structures for efficacy and functionality for applications in the chemical industries. Saponin derivatives will be engineered using a synthetic biology tool kit and metabolic pathway expression in a Nicotiana benthamiana host. Extracted saponins will be evaluated for physical properties and in application screens. Structure-function rules will be built to iteratively optimise the saponin structures for functionality. The options for a biotechnology platform, scale-up and commercial production of the lead saponins will be assessed.

皂苷已经在饮料、食品和化妆品工业中发现作为发泡剂的工业应用。然而，三萜皂苷结构的广度尚未被充分探索或开发，并且它们的应用受限于来自植物提取物的可及性和可用性，依赖于文献中记载的广泛的物理和生物活性性质。该项目将展示三萜皂苷结构的优化，用于化学工业的功效和功能。皂苷衍生物将使用合成生物学工具包和代谢途径在本氏烟草宿主中表达来工程化。将评价提取的皂苷的物理性质和应用筛选。将建立结构-功能规则，以迭代优化皂苷结构的功能。将评估生物技术平台、扩大规模和商业化生产先导皂苷的备选方案。

项目成果

Building foundations for an open perspective on synthetic biology research and innovation.

为合成生物学研究和创新的开放视角奠定基础。

• DOI: 10.1111/nph.12361
• 发表时间: 2013
• 期刊: The New phytologist
• 作者: Rant J