MARIHUANA AND MALE NEUROENDOCRINE AND SEXUAL FUNCTIONS

大麻与男性神经内分泌和性功能

• 批准号: 3208660
• 负责人: Andrzej Bartke
• 金额: $ 12.86万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-09-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3208660
• 关键词: Cannabis; androgens; animal age group; animal developmental psychology; cannabinoids; cholesterol; dosage; drug addiction antagonist; drug interactions; drug metabolism; electrostimulus; endocrine pharmacology; experimental brain lesion; fertility; gonadotropin releasing factor; hamsters; hormone receptor; hypophysectomy; hypothalamus; immature animal; laboratory mouse; laboratory rabbit; laboratory rat; male; naloxone; neuroendocrine system; neuropharmacology; neurotransmitter metabolism; pituitary gonadal axis; psychotropic drugs; radioimmunoassay; radiotracer; reproduction; sex behavior; testis; testosterone

We are studying the effects of marihuana on male endocrine, reproductive and sexual functions. Our objective is to define the effects of psychoactive and non-psychoactive constituents of marihuana on the pituitary, the testes and on male sexual behavior and to determine the mechanisms of these effects. During the next four years, we will address the mechanism of action of delta-9-tetrahydrocannabinol (THC) on male copulatory behavior and study the effects of perinatal or adult administration of THC, cannabinol (CBN) and cannabidiol (CBD) on hypothalamic and pituitary function. More specifically, we will test our hypothesis that the neurotransmitter-mediated alterations in the release of gonadotropin releasing hormone (GnRH) are responsible for the effects of THC on sexual behavior. We will also examine the effects of THC, CBN and CBD on the function of hypothalamic catecholaminergic neurons which are known to control the release of gonadotropins and prolactin from the anterior pituitary. These studies will be conducted in male rats and mice and will involve oral administration of different components of marihuana, measurements of levels and turnover rates of hypothalamic neurotransmitters, incubations of hypothalami and anterior pituitaries, determinations of GnRH receptors in the pituitary and radioimmunoassay measurements of blood levels of pituitary and testicular hormones. Copulatory behavior will be tested in the presence of ovariectomized females brought into sexual receptivity by estrogen and progesterone injections. Further studies will involve administration of blockers and precursors of hypothalamic neurotransmitters in an attempt to mimic or prevent cannabinoid effects on behavior and pituitary function as well as cuts, lesions and stimulations of specific sites in the brain aimed at localizing the sites of cannabinoid action and the anatomical pathways of their effects.

我们正在研究大麻对男性内分泌、生殖 性功能。 我们的目标是确定 大麻的精神活性和非精神活性成分 垂体，睾丸和男性性行为，并确定 这些影响的机制。 在接下来的四年里，我们将 δ-9-四氢大麻酚（THC）对男性作用机制 交配行为和研究围产期或成人的影响 THC，大麻酚（CBN）和大麻二酚（CBD）的施用 下丘脑和垂体功能。 更具体地说，我们将测试 假设神经递质介导的释放的改变 促性腺激素释放激素（GnRH）是负责的影响， 四氢大麻酚对性行为的影响。 我们亦会研究THC、CBN及 CBD对下丘脑儿茶酚胺能神经元功能的影响 已知其控制促性腺激素和催乳素从 前叶 这些研究将在雄性大鼠和小鼠中进行 并且将包括口服施用大麻的不同组分， 下丘脑的水平和周转率的测量 神经递质，下丘脑和前垂体的孵化， 垂体促性腺激素释放激素受体测定及放射免疫分析 测量垂体和睾丸激素的血液水平。 交配行为将在卵巢切除的 雌激素和孕激素使雌性进入性接受状态 注射剂 进一步的研究将涉及给予阻滞剂， 下丘脑神经递质的前体，试图模仿或 防止大麻素对行为和垂体功能的影响， 脑内特定部位的切割、损伤和刺激， 定位大麻素作用的位点和 他们的影响。

项目成果

Evidence for a direct anterior pituitary site of delta-9-tetrahydrocannabinol action.

δ-9-四氢大麻酚直接作用于垂体前叶的证据。

• DOI: 10.1016/0091-3057(91)90370-h
• 发表时间: 1991
• 期刊: Pharmacology, biochemistry, and behavior
• 作者: Murphy,LL;Newton,SC;Dhali,J;Chávez,D
• 通讯作者: Chávez,D

Metabolomic diferences between COVID-19 and H1N1 influenza induced ARDS.

• DOI: 10.1186/s13054-021-03810-3
• 发表时间: 2021-11-15
• 期刊: Critical care (London, England)
• 作者: Lorente JA;Nin N;Villa P;Vasco D;Miguel-Coello AB;Rodriguez I;Herrero R;Peñuelas O;Ruiz-Cabello J;Izquierdo-Garcia JL
• 通讯作者: Izquierdo-Garcia JL

Effects of delta-9-tetrahydrocannabinol, cannabinol and cannabidiol, alone and in combinations, on luteinizing hormone and prolactin release and on hypothalamic neurotransmitters in the male rat.

δ-9-四氢大麻酚、大麻酚和大麻二酚单独和组合对雄性大鼠黄体生成素和催乳素释放以及下丘脑神经递质的影响。

• DOI: 10.1159/000125604
• 发表时间: 1990
• 期刊: Neuroendocrinology
• 影响因子: 4.1
• 作者: Murphy,LL;Steger,RW;Smith,MS;Bartke,A
• 通讯作者: Bartke,A

Benchtop nuclear magnetic resonance-based metabolomic approach for the diagnosis of bovine tuberculosis.

基于台式核磁共振的代谢组学方法用于诊断牛结核病。

• DOI: 10.1111/tbed.14365
• 发表时间: 2022
• 期刊: Transboundary and emerging diseases
• 影响因子: 4.3
• 作者: Ruiz-Cabello,Jesús;Sevilla,IkerA;Olaizola,Ekine;Bezos,Javier;Miguel-Coello,AnaB;Muñoz-Mendoza,Marta;Beraza,Marta;Garrido,JosebaM;Izquierdo-García,JoseL
• 通讯作者: Izquierdo-García,JoseL

In vitro effects of psychoactive and non-psychoactive cannabinoids on immature rat Sertoli cell function.

精神活性和非精神活性大麻素对未成熟大鼠支持细胞功能的体外影响。

• DOI: 10.1016/0024-3205(93)90585-q
• 发表时间: 1993
• 期刊: Life sciences
• 影响因子: 6.1
• 作者: Newton,SC;Murphy,LL;Bartke,A
• 通讯作者: Bartke,A