CELL BIOLOGY OF PERIODONTAL LIGAMENT FIBROBLASTS

牙周韧带成纤维细胞的细胞生物学

• 批准号: 3219898
• 负责人: MOON IL CHO
• 金额: $ 13.96万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-02-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219898
• 关键词: autoradiography; cell cell interaction; cell differentiation; cytoplasm; electron microscopy; fibroblasts; freeze etching; immunochemistry; intracellular membranes; membrane activity; osteoblasts; periodontium; phagocytosis; secretion

Fibroblasts are the predominant cells and collagen is the most abundant extracellular component in the periodontal ligament (PDL). The relationship between fibroblast polarity and orientation and the genesis of the 3-D architecture of the extracellular matrix is of increasing interest to cell biologists. Furthermore, the interrelationship between cytostructure and function during collagen secretion and fibrillogenesis contains information of significance not only to cell biology but also to a better understanding of the biology of the PDL. Greater understanding of PDL fibroblasts is of general importance to research in periodontal health. We propose to conduct research into six areas of fibroblast activity: 1) Studies of the cytological and synthetic aspects of fibroblast differentiation in relation to the development of the PDL fiber bundles. 2) Ultrastructural immunolocalization of C- and N- propeptides of collagen in fibroblasts and fibrils so as to learn more about their role in fibrillogenesis. 3) Immunocytochemical studies of collagen types I, III, V and fibronectin during fibroblast differentiation and PDL development. 4)\Studies of the relationship between structure and function of the Golgiapparatus of PDL fibroblasts. 5) The localization of receptors for insulin, growth hormone and fibroblast growth factor on PDL fibroblast membranes during cytodifferentiation and PDL development. 6) Immunocytochemical localization of cathepsin D in periodontal ligament fibroblasts at the ultrastructural level.

成纤维细胞是最主要的细胞，胶原蛋白最丰富 牙周膜细胞外成分(PDL)。这个 成纤维细胞的极性和取向与牙周炎发生的关系 细胞外基质的三维结构越来越引起人们的兴趣 对细胞生物学家来说。此外，两国之间的相互关系 胶原分泌和纤维形成过程中的细胞结构和功能 包含的信息不仅对细胞生物学有重要意义，而且对 对PDL的生物学有更好的了解。更好地理解 牙周膜成纤维细胞对牙周健康的研究具有普遍意义。 我们建议对成纤维细胞活性的六个方面进行研究：1) 成纤维细胞的细胞学和合成方面的研究 分化与PDL纤维束发育的关系。 2)胶原C-和N-前肽的超微结构免疫定位 从而更多地了解它们在细胞中的作用 纤维形成。3)I、III、V型胶原的免疫细胞化学研究 纤维连接蛋白在成纤维细胞分化和PDL发育中的作用。 4)结构与功能关系的研究 PDL成纤维细胞的高尔基体。5)受体的定位 胰岛素、生长激素和成纤维细胞生长因子对PDL成纤维细胞的影响 细胞分化和PDL发育过程中的细胞膜。6) 组织蛋白酶D在牙周膜中的免疫细胞化学定位 超微结构水平的成纤维细胞。