CELL BIOLOGY OF PERIODONTAL LIGAMENT FIBROBLAST

牙周韧带成纤维细胞的细胞生物学

• 批准号: 3219900
• 负责人: MOON IL CHO
• 金额: $ 11.38万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-02-01 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219900
• 关键词: autoradiography; cell cell interaction; cytoplasm; electron microscopy; fibroblasts; freeze etching; intracellular membranes; membrane activity; osteoblasts; periodontium; phagocytosis; secretion

The over all objective to be pursued will be to gain a more complete understanding of the structure relationships of the periodontal ligament (PDL) fibroblasts and their totipotential role in maintaining the periodontal apparatus. Emphasis will be directed to further characterization of the PDL fibroblast cell surface using the freeze-fracture technique. Special emphasis will be devoted to determining the nature, and distribution of cell-to-matrix and cell-to-cell contacts. The relationship of the subsurface cytoplasmic filaments to membrane particles and cell-to-matrix contacts will be investigated by using deep etching techniques. The particle distribution of cytoplasmic membranes will be established as a means of characterizing various cytoplasmic domains in relation to the secretory process. Having previously carried out detailed electron microscopic autoradiographic (EM-ARG) studies of collagen secretion by PDL fibroblasts in vivo, it is appropriate that similar EM-ARG studies of the synthesis of non-collagenous matrix proteins be carried out. Con A and cationic ferritin binding to PDL fibroblasts will be studied and correlated to freeze-fracture IMP distributions and the EM-ARG results of glycoprotein secretion. Moreover, the activity of PDL fibroblasts adjacent to sites of bone resorption will be examined for collagen phagocytosis, synthesis and secretion and ability to differentiate into new osteoblasts. Finally, the ultrastructural features and synthetic capacity of PDL fibroblasts will be studied in early, established and advanced lesions of periodontal disease and compared to normal PDL fibroblasts.

要追求的总体目标将是获得更完整的 对牙周膜结构关系的认识 (PDL)成纤维细胞及其在维持动脉粥样硬化中的潜在作用 牙周器。重点将指向进一步 人牙周膜成纤维细胞表面的光镜观察 冷冻断裂技术。特别强调将致力于确定 细胞-基质和细胞-细胞接触的性质和分布。 亚表层细胞质细丝与膜的关系 粒子和细胞与基质的接触将通过使用深度分析进行研究 蚀刻技术。细胞质膜的颗粒分布 将被确立为一种表征各种细胞质的手段 与分泌过程有关的结构域。之前曾携带 详细的电子显微镜放射自显影(EM-ARG)研究 体内PDL成纤维细胞分泌胶原蛋白的研究 非胶原基质蛋白合成的类似EM-ARG研究 被执行。ConA和阳离子铁蛋白与PDL成纤维细胞的结合 将被研究并与冻裂IMP分布和 EM-ARG结果显示糖蛋白分泌。此外，PDL的活性 骨吸收部位附近的成纤维细胞将被检查 胶原蛋白的吞噬、合成、分泌和分化能力 转化为新的成骨细胞。最后，超微结构特征和合成 PDL成纤维细胞的能力将在早期、建立和 牙周病晚期病变与正常牙周病的比较 成纤维细胞。