CELL BIOLOGY OF PERIODONTAL LIGAMENT FIBROBLAST

要追求的总目标将是获得一个更完整的 了解牙周膜的结构关系 (PDL)成纤维细胞及其在维持 牙周器 重点将进一步 PDL成纤维细胞表面的表征 冷冻断裂技术 特别强调将致力于确定 细胞与基质和细胞与细胞接触的性质和分布。 亚表面细胞质丝与细胞膜的关系 颗粒和细胞与基质的接触将通过使用深 蚀刻技术 细胞质膜的颗粒分布 将被建立为表征各种细胞质的手段， 与分泌过程有关的结构域。 此前， 详细的电子显微镜放射自显影（EM-ARG）研究 体内PDL成纤维细胞的胶原蛋白分泌， 非胶原基质蛋白合成的类似EM-ARG研究 执行。 Con A和阳离子铁蛋白与PDL成纤维细胞的结合 将被研究和相关的冻结断裂IMP分布和 糖蛋白分泌的EM-ARG结果。 此外，PDL的活性 将检查骨吸收部位附近的成纤维细胞， 胶原蛋白的吞噬、合成和分泌以及分化能力 转化成新的成骨细胞 最后，研究了其超微结构特征和合成方法。 PDL成纤维细胞的能力将在早期，建立和 牙周病晚期病变，并与正常PDL相比 成纤维细胞

The over all objective to be pursued will be to gain a more complete understanding of the structure relationships of the periodontal ligament (PDL) fibroblasts and their totipotential role in maintaining the periodontal apparatus. Emphasis will be directed to further characterization of the PDL fibroblast cell surface using the freeze-fracture technique. Special emphasis will be devoted to determining the nature, and distribution of cell-to-matrix and cell-to-cell contacts. The relationship of the subsurface cytoplasmic filaments to membrane particles and cell-to-matrix contacts will be investigated by using deep etching techniques. The particle distribution of cytoplasmic membranes will be established as a means of characterizing various cytoplasmic domains in relation to the secretory process. Having previously carried out detailed electron microscopic autoradiographic (EM-ARG) studies of collagen secretion by PDL fibroblasts in vivo, it is appropriate that similar EM-ARG studies of the synthesis of non-collagenous matrix proteins be carried out. Con A and cationic ferritin binding to PDL fibroblasts will be studied and correlated to freeze-fracture IMP distributions and the EM-ARG results of glycoprotein secretion. Moreover, the activity of PDL fibroblasts adjacent to sites of bone resorption will be examined for collagen phagocytosis, synthesis and secretion and ability to differentiate into new osteoblasts. Finally, the ultrastructural features and synthetic capacity of PDL fibroblasts will be studied in early, established and advanced lesions of periodontal disease and compared to normal PDL fibroblasts.