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CELL BIOLOGY OF PERIODONTAL LIGAMENT FIBROBLASTS

牙周韧带成纤维细胞的细胞生物学

基本信息

批准号：
3219902
负责人：
MOON IL CHO
金额：
$ 14.95万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-02-01 至 1989-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3219902
关键词：
autoradiography cell cell interaction cell differentiation collagen cytoplasm electron microscopy fibroblasts freeze etching immunochemistry intracellular membranes membrane activity osteoblasts periodontium phagocytosis secretion

项目摘要

Fibroblasts are the predominant cells and collagen is the most abundant extracellular component in the periodontal ligament (PDL). The relationship between fibroblast polarity and orientation and the genesis of the 3-D architecture of the extracellular matrix is of increasing interest to cell biologists. Furthermore, the interrelationship between cytostructure and function during collagen secretion and fibrillogenesis contains information of significance not only to cell biology but also to a better understanding of the biology of the PDL. Greater understanding of PDL fibroblasts is of general importance to research in periodontal health. We propose to conduct research into six areas of fibroblast activity: 1) Studies of the cytological and synthetic aspects of fibroblast differentiation in relation to the development of the PDL fiber bundles. 2) Ultrastructural immunolocalization of C- and N- propeptides of collagen in fibroblasts and fibrils so as to learn more about their role in fibrillogenesis. 3) Immunocytochemical studies of collagen types I, III, V and fibronectin during fibroblast differentiation and PDL development. 4)\Studies of the relationship between structure and function of the Golgiapparatus of PDL fibroblasts. 5) The localization of receptors for insulin, growth hormone and fibroblast growth factor on PDL fibroblast membranes during cytodifferentiation and PDL development. 6) Immunocytochemical localization of cathepsin D in periodontal ligament fibroblasts at the ultrastructural level.

成纤维细胞是主要的细胞，胶原蛋白是最丰富的牙周膜（PDL）中的细胞外成分。的成纤维细胞极性和方向与成纤维细胞发生的关系细胞外基质的三维结构越来越受到关注细胞生物学家。此外，胶原蛋白分泌和原纤维形成期间的细胞结构和功能包含的信息不仅对细胞生物学，更好地了解PDL的生物学。更好地了解牙周膜成纤维细胞在牙周健康研究中具有重要意义。我们建议对成纤维细胞活性的六个方面进行研究：1）成纤维细胞的细胞学和合成研究分化与PDL纤维束的发育有关。 2)胶原蛋白C、N前肽的超微结构免疫定位在成纤维细胞和原纤维中，以便更多地了解它们在纤维形成 3)Ⅰ、Ⅲ、Ⅴ型胶原的免疫细胞化学研究和纤维连接蛋白。（4）研究了细胞的结构与功能的关系。 PDL成纤维细胞的高尔基体。 5)受体的定位胰岛素、生长激素和成纤维细胞生长因子对牙周膜成纤维细胞影响细胞分化和PDL发育过程中的膜。六、牙周膜组织蛋白酶D的免疫细胞化学定位成纤维细胞在超微结构水平。