BBSRC Embrapa - Understanding the importance of the wheat root microbiome for sustainable crop production.

BBSRC Embrapa - 了解小麦根微生物组对可持续作物生产的重要性。

• 批准号: BB/N004418/1
• 负责人: Tim Mauchline
• 金额: $ 3.67万
• 依托单位: Rothamsted Research
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN004418%2F1
• 关键词: BBSRC; Embrapa; Understanding; importance; wheat

The UK team will be comprised of 3 Rothamsted based scientists: Dr. Tim Mauchline (UK Principal Investigator), Prof. Penny Hirsch and Mr. Ian Clark. The Brazilian team from Embrapa environment is composed of three scientists: Dr. Rodrigo Mendes (Brazil Principal Investigator), Dr. Vanessa Nessner and Dr. Maike Rossmann. The complete team have complementary and overlapping skill sets necessary to perform rhizosphere metagenomics and wheat microcosm experiments.UK teamDr. Mauchline is a microbiologist with expertise in rhizosphere microbiology and microcosms of various crops including wheat. Prof. Hirsch is a soil microbiologist with a wealth of experience in soil nutrient cycling. Mr. Ian Clark is a microbiologist with bioinformatics skills. Brazil teamDr. Mendes is a molecular microbiologist with skills in metagenomics of agricultural systems. Dr. Nessner is a molecular biologist with bioinformatics skills. Dr. Maike Rossman a soil microbiologist with experience of wheat cultivation.This pump-priming proposal aims to continue the collaborative links established by the visit of Dr. Tim Mauchline to Dr. Rodrigo Mendes' laboratory at Embrapa Environment for three weeks in May 2014, funded by an ISIS award. Dr. Mauchline's visit proved very useful as it allowed him to become embedded within the Embrapa group. Many discussions were held between Dr. Mauchline and Dr. Mendes, as well with other members of the unit, to determine future directions of microbiome research in the rhizosphere with respect to wheat yields. As a direct result of the visit, a post-doctoral scientist based at Embrapa Environment, Dr. Vanessa Nessner, has joined the Rothamsted team for a period of five months (Dec- May 2015) to work on the wheat microbiome in healthy and diseased plants, and her work will provide a platform for the pump-priming research. Additionally, Dr. Maike Rossmann, who spent a one year post-doctoral position with the Rothamsted team, is now based in Dr. Mendes' laboratory at Embrapa environment working on the metagenomics of the wheat rhizosphere in different soil types. As such, we have developed a functioning team unit between the Rothamsted and Embrapa environment microbiology labs, with complementary skills, which this proposal hopes to build on.The main field experiment has already been setup at Rothamsted, and will require no extra costs as permission has been granted to sample it. The research costs are for soil DNA extractions kits, amplicon sequencing and a comparative pot experiment. Please see the JoR for more information. Wheat sampling, soil DNA extractions and pot study analyses will be a collaborative effort as the visiting Embrapa Brazilian scientists and the Rothamsted team will conduct these together in the Rothamsted field trial and labs during their one month visit to the UK. The main costs requested will be for partners to travel between Brazil and the UK. Dr. Mendes, Dr. Rossmann and Dr. Nessner will travel to the UK to discuss and write the full grant proposal, in addition to performing the collaborative field and lab work described above. We will also invite a further three scientists to provide advice (Prof. Phil Poole and Dr. Gary Bending from the UK, and Dr. Fernando Andreote from Brazil) in a small-scale workshop. It is proposed that Dr. Mauchline, Prof. Penny Hirsch and Mr. Ian Clark from the Rothamsted team will also travel to Embrapa environment in order to further discussions, and to draft a manuscript from the preliminary data with the Brazil team.

英国团队将由3位洛桑研究所的科学家组成：Tim Mauchline博士（英国首席研究员）、Penny Hirsch教授和Ian Clark先生。来自Embrapa环境的巴西团队由三位科学家组成：Rodrigo Mendes博士（巴西首席研究员），Vanessa Nessner博士和Maike Rossmann博士。整个团队具有进行根际宏基因组学和小麦微观世界实验所需的互补和重叠技能。英国teamDr。Mauchline是一位微生物学家，在包括小麦在内的各种作物的根际微生物学和微观世界方面具有专业知识。赫希教授是一位土壤微生物学家，在土壤养分循环方面有着丰富的经验。Ian Clark先生是一位具有生物信息学技能的微生物学家。巴西teamDr。门德斯是一名分子微生物学家，精通农业系统宏基因组学。他是一位具有生物信息学技能的分子生物学家。Maike Rossman博士是一位具有小麦种植经验的土壤微生物学家。2014年5月，Tim Mauchline博士访问了Embrapa Environment的Rodrigo Mendes博士的实验室，该项目由ISIS资助，旨在继续建立合作关系。Mauchline博士的访问被证明非常有用，因为这让他融入了Embrapa团队。Mauchline博士和Mendes博士以及该小组的其他成员进行了多次讨论，以确定根际微生物组研究与小麦产量有关的未来方向。此次访问的直接结果是，Embrapa Environment的博士后科学家Vanessa Nessner博士加入了Rothamsted团队，为期五个月（2015年12月至5月），研究健康和患病植物的小麦微生物组，她的工作将为泵启动研究提供一个平台。此外，Maike Rossmann博士在Rothamsted团队度过了一年的博士后职位，现在在Embrapa环境的Mendes博士的实验室工作，研究不同土壤类型下小麦根际的宏基因组学。因此，我们在洛桑研究所和Embrapa环境微生物实验室之间建立了一个功能齐全的团队单位，具有互补的技能，本提案希望以此为基础。主要的田间试验已经在洛桑研究所完成，并且由于已经获得取样许可，将不需要额外的费用。研究费用包括土壤DNA提取试剂盒、扩增子测序和比较盆栽实验。请参阅JoR了解更多信息。小麦取样、土壤DNA提取和盆栽研究分析将是一项合作工作，来访的巴西Embrapa公司的科学家和洛桑研究所的团队将在他们对英国为期一个月的访问期间，在洛桑研究所的田间试验和实验室中共同进行这些工作。所需的主要费用将用于合作伙伴在巴西和英国之间的旅行。Mendes博士、Rossmann博士和nesner博士将前往英国讨论并撰写完整的资助提案，此外还将进行上述领域和实验室的合作工作。我们还将邀请另外三位科学家（来自英国的Phil Poole教授和Gary bender博士以及来自巴西的Fernando Andreote博士）在一个小型研讨会上提供建议。建议来自Rothamsted团队的Mauchline博士，Penny Hirsch教授和Ian Clark先生也将前往Embrapa环境进行进一步讨论，并根据巴西团队的初步数据起草一份手稿。

项目成果

Inorganic Nitrogen Application Affects Both Taxonomical and Predicted Functional Structure of Wheat Rhizosphere Bacterial Communities.

• DOI: 10.3389/fmicb.2018.01074
• 发表时间: 2018
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Kavamura VN;Hayat R;Clark IM;Rossmann M;Mendes R;Hirsch PR;Mauchline TH
• 通讯作者: Mauchline TH