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BBSRC Embrapa - Developing mitochondrial mediated resilience to abiotic stress during plant reproduction.

BBSRC Embrapa - 开发植物繁殖过程中线粒体介导的非生物胁迫恢复能力。

基本信息

批准号：
BB/N004523/1
负责人：
Zoe Wilson
金额：
$ 7.45万
依托单位：
University of Nottingham
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2015
资助国家：
英国
起止时间：
2015 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FN004523%2F1
关键词：
BBSRC Embrapa Developing mitochondrial mediated

项目摘要

This project will bring together groups from the UK and Brazil who are working on different aspects of heat and drought stress, pollen development and mitochondrial function in wheat to address the molecular genetic and physiological events occurring during reproductive heat and drought stress. This will enable development of germplasm with enhanced resilience to environmental stress during flowering.University of Nottingham(UoN)-Prof ZA Wilson: Reproductive development and male fertility in wheat. Expertise in the molecular regulation of pollen development and translation of gene networks from models, to crops. This has identified conserved transcription factors, which are being explored as tools to control crop fertility for hybrid breeding. Investigation of flowering adaptations in wheat associated with flowering time, stamen structure and anther dehiscence. The impact of temperature stress of pollen development, with the target of identifying traits for resilience during temperature stress.-Prof I King & Dr J King: Ancestral Introgression Programme to transfer genetic variation into wheat from its distant relatives, by transferring the entire genome of a range of species, e.g. Secale cereale, Aegilops speltoides, Ambylopyrum muticum, Triticum urartu, Thinopyrum bessarabicum, Th. elongatum, Th. intermedium, Th. ponticum, T. timopheevii, into wheat in small overlapping chromosome segments. While still at a preliminary stage derivatives carrying introgressions from this programme are already under study for a wide range of traits, e.g. tolerance to abiotic stress, chemical composition, morphological traits.-Assoc Prof J Foulkes: Resource-use efficiency in wheat: Expertise in wheat physiology- focusing on ear fertility and resource-use efficiency. Includes (i) physiological and genetic basis of ear fertility; thresholds for assimilate partitioning to the ear and alternative sinks. Novel ear-fertility genes related to increased efficiency of tillering, spikelet primordia number and floret fertility (ii) physiological and genetic basis of N-use efficiency and (iii) water-use efficiency.Brazilian Researchers - Universidade Estadual de CampinasExpertise in Molecular Genetic manipulation of Mitochondria.Paulo Arruda - (PI) Molecular biology, genetics, genomics,Natalia Verza - (post-doc) Molecular biology, metabolic engeneeringPedro Barreto - (pos-doc) Molecular Biology, genomics, mitochondrial metabolismJuliana Yassitepe - (Embrapa researcher) GeneticsGeraldo Cançado - (Embrapa researcher) Transgenic approaches.Infrastructure:-Biosciences, UoN has excellent research facilities, including purpose built molecular labs, imaging facilities with state-of-the art Micro-CT Imaging Facility, Controlled Environment facilities; the research environment is conducive to cutting-edge research, in a collaborative, interdisciplinary environment. ZAW is part of the Centre for Plant Integrative Biology (CPIB), a major international centre to develop dynamic and multiscale models of plant development, involving data capture on dynamic hormone fluxes; cell & organ growth; gene expression, protein & metabolite profiles and tissue mechanics. CPIB will provide added support for data analysis, modeling and provide a platform for data dissemination.

该项目将汇集来自英国和巴西的研究小组，他们正在研究高温和干旱胁迫、小麦花粉发育和线粒体功能的不同方面，以解决生殖高温和干旱胁迫期间发生的分子遗传和生理事件。这将使种质资源在开花期间对环境胁迫的适应能力增强。诺丁汉大学- ZA Wilson教授：小麦的生殖发育和雄性生育力。专门研究花粉发育的分子调控和基因网络从模型到作物的翻译。这已经确定了保守的转录因子，这些转录因子正在被探索作为控制杂交育种作物肥力的工具。小麦开花适应性与开花时间、雄蕊结构和花药开裂的关系研究。研究温度胁迫对花粉发育的影响，以确定在温度胁迫下花粉的抗逆性。- I King教授和J King博士：祖先渗透计划，通过转移一系列物种的整个基因组，将遗传变异从其远亲转移到小麦中，例如：黑麦、鹅毛豆、Ambylopyrum muticum、乌拉尔小麦、Thinopyrum bessarabicum、Th。elongatum Th。媒介,Th。在小的重叠染色体片段中转化成小麦。虽然仍处于初步阶段，从该计划携带渗入的衍生物已经在广泛的性状研究中，例如对非生物胁迫的耐受性，化学成分，形态性状。- J Foulkes副教授：小麦资源利用效率：小麦生理学专业知识-关注穗肥力和资源利用效率。包括(i)耳部生育的生理和遗传基础；同化物分配到耳朵和替代汇的阈值。与分蘖效率、小穗原基数量和小花育性提高有关的穗育性新基因（ii）氮利用效率的生理和遗传基础（iii）水分利用效率。巴西研究人员——坎皮纳大学的线粒体分子遗传操作专家。Paulo Arruda - (PI)分子生物学，遗传学，基因组学，Natalia Verza -（博士后）分子生物学，代谢工程pedro Barreto -（博士后）分子生物学，基因组学，线粒体代谢juliana Yassitepe - （Embrapa研究员）遗传学geraldo canado - （Embrapa研究员）转基因方法。基础设施：生物科学：联合国大学拥有一流的研究设施，包括专门建造的分子实验室、配备最先进的微型ct成像设备的成像设施、受控环境设施；研究环境有利于前沿研究，在一个协作的，跨学科的环境。ZAW是植物综合生物学中心（CPIB）的一部分，该中心是一个主要的国际中心，开发植物发育的动态和多尺度模型，涉及动态激素通量的数据捕获；细胞和器官生长；基因表达，蛋白质和代谢物谱和组织力学。CPIB将为数据分析、建模提供额外支持，并为数据传播提供平台。