BBSRC Embrapa: Temperature resilience of flowering in UK and Brazilian wheat (TempRe)

该项目涉及诺丁汉大学Rothamsted Research和EmbRapa Trigo。这两个团队在非生物胁迫耐受性的遗传控制、花形成的发育遗传学和代谢信号系统方面拥有专业知识。它们带来了一系列互补的技术，从系统生物学和生物信息学，到遗传学和生理学，再到小麦转化。这三个机构都装备精良，设施齐全，可用于研究的各个方面。其目的是利用分子、全株和转基因方法研究英国和巴西小麦在孕穗期和开花期对温度胁迫的反应。英国团队：Nigel Halford教授，RRes：作物代谢调节的遗传学专业知识，以及代谢如何受到非生物胁迫的影响，使用的技术范围从代谢网络的数学模型、信号因子的分子和生化分析到RNAseq和GC-MS分析。2013年访问EmbRapa Trigo。Matthew Paul博士，RRes：海藻糖6-磷酸信号转导，糖信号在非生物胁迫反应中的作用，以及在胁迫条件下提高产量的操作方面的专业知识。使用了一系列分子和生理技术，以及创新的化学。RRES的Huw Jones教授：领导RRes的小麦转化，利用基因上调、RNAi沉默和基因组编辑，并验证了30种不同的基因启动子。2011年参加了BBSRC/EmbRapa小麦改良研讨会，并将于2015年在第十五届巴西植物生理学大会上发表全体演讲。拥有转基因风险评估和转基因田间管理经验。诺丁汉大学佐伊·威尔逊教授：植物发育遗传学方面的专业知识，特别是花粉和花药发育的调控。目前的研究涉及将花粉基因网络从模型转移到小麦和大麦，以及拟南芥、小麦和班巴拉坚果等作物花粉发育过程中的温度胁迫，这些作物表现出对极端温度的耐受性。诺丁汉大学约翰·福克斯教授：决定小麦抗逆性的性状专业知识。目前的研究包括高通量遥感表型的发展、穗育性的生理和遗传基础以及控制分蘖、小穗原基数量和小花育性的新基因的鉴定。2013年访问恩布拉帕·特里戈。巴西队：埃迪娜·莫雷斯科博士。EmbRapa Trigo：小麦育种方面的专业知识，特别是数量遗传学、经典育种技术、GxE和植物/病原菌互作，重点是评估和选择耐非生物逆境的植物。生物信息学专业知识应用于鉴定分子标记和分析与生物和非生物胁迫相关的小麦转录组数据。Elene Yamazaki Lau博士：分子生物学和生物技术专业知识；目前正在开发根癌农杆菌介导的小麦遗传转化协议，以便培育出对非生物胁迫(包括高温)具有更强耐受性的品系。豪尔赫·费尔南多·佩雷拉博士：在小麦和大麦非生物胁迫反应的分子基础方面的专业知识；还对开发小麦和大麦的转化技术感兴趣。卢西亚诺·康索利博士：分子遗传学和小麦预育种方面的专门知识；利用联合遗传学和QTL研究来研究非生物胁迫反应和收获前发芽。毛罗·塞萨尔·塞拉罗·特谢拉博士：在恶劣环境下支撑小麦品种抗逆性和产量潜力的机制方面的专门知识。奥斯玛·罗德里格斯博士：植物生理学方面的专门知识；目前正在研究巴西南部冬季生长期小麦产量形成和籽粒形成的生理基础。Aretha Arcen P.Correa，博士生

The project involves Rothamsted Research, University of Nottingham, and Embrapa Trigo. Between them the teams have expertise in the genetic control of abiotic stress tolerance, developmental genetics of flower formation, and metabolic signalling systems. They bring a range of complementary techniques, from systems biology and bioinformatics through genetics and physiology to wheat transformation. All three institutions are well-equipped, with facilities for all aspects of the study in place. The aim is to study the response of UK and Brazilian wheat to temperature stress between booting and flowering using molecular, whole-plant and transgenic approaches.UK team:Prof. Nigel Halford, RRes: Expertise in the genetics of metabolic regulation in crop plants and how metabolism is affected by abiotic stress, using techniques ranging from mathematical modelling of metabolic networks through molecular and biochemical analyses of signalling factors to RNAseq and GC-MS analyses. Visited Embrapa Trigo in 2013.Dr Matthew Paul, RRes: Expertise in trehalose 6-phosphate signalling, the role of sugar signalling in responses to abiotic stresses, and manipulation to improve yield under stress conditions. Uses a range of molecular and physiological techniques, as well as innovative chemistry. Prof Huw Jones, RRes: Leads wheat transformation at RRes, exploiting gene up-regulation, RNAi silencing and genome editing and validating 30 different gene promoters. Participated in the BBSRC/Embrapa workshop on wheat improvement in 2011 and will deliver a plenary lecture in the XV Brazilian Congress of Plant Physiology in 2015. Has experience of GMO risk assessment and GM field management.Professor Zoe Wilson, University of Nottingham: Expertise in plant developmental genetics, specifically the regulation of pollen and anther development. Current research involves the translation of pollen gene networks from models to wheat and barley, and temperature stress during pollen development in Arabidopsis, wheat and crops such as Bambara Ground Nut, which show tolerance to extreme temperatures.Prof John Foulkes, University of Nottingham: Expertise in the traits determining stress tolerance in wheat. Current research includes the development of high-throughput remote sensing phenotyping, the physiological and genetic basis of ear fertility and the identification of novel genes controlling tillering, spikelet primordia number and floret fertility. Visited Embrapa Trigo in 2013.Brazil team: Dr Edina Moresco. Embrapa Trigo: Expertise in wheat breeding, specifically quantitative genetics, classical breeding techniques, G x E and plant/pathogen interactions, with a focus on evaluation and selection of plants for abiotic stress tolerance. Dr Antonio Nhani Jr, Embrapa Trigo: Expertise in bioinformatics applied to the identification of molecular markers and the analysis of wheat transcriptome data related to biotic and abiotic stresses.Dr Elene Yamazaki Lau: Expertise in molecular biology and biotechnology; currently developing A. tumefaciens-mediated genetic transformation protocols for wheat in order to generate lines with increased tolerance to abiotic stresses, including heat. Dr Jorge Fernando Pereira: Expertise in the molecular basis of abiotic stress responses in wheat and barley; also has interests in developing wheat and barley transformation techniques. Dr Luciano Consoli: Expertise in molecular genetics and wheat pre-breeding; investigating abiotic stress responses and pre-harvest sprouting using association genetics and QTL studies.Dr Mauro Cesar Celaro Teixeira: Expertise in the mechanisms underpinning stress tolerance and yield potential of wheat cultivars in harsh environments.Dr Osmar Rodrigues: Expertise in plant physiology; currently studying the physiological bases for yield formation and grain set on wheat during the winter growing season in the Southern part of Brazil.Aretha Arcen P. Correa, PhD student