STUDIES ON THE DEXTRANSUCRASE OF STREPTOCOCCUS SANGUIS

血链球菌葡聚糖蔗糖酶的研究

• 批准号: 3218905
• 负责人: ROBERT MAYER
• 金额: $ 9.3万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-01-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3218905
• 关键词: Streptococcus sanguis; carbohydrate biosynthesis; chemical reaction; chromatography; dental caries; dental plaque; detergents; dextrans; enzyme structure; glycosyltransferase; oral bacteria; polymers; radiotracer; scintillation counter; tissue /cell culture

Dextransucrase catalyzes the formation of a glucan containing Alpha 1-6 and Alpha 1-3 bonds. The glucan, dextran, is a major component of dental plaque and is formed by the action of the enzyme on sucrose. Studies are proposed that will extend work that is currently in progress. Elements of the reaction pathway will be studied to evaluate the physiological importance of two alternate pathways of polymer formation. Similarly, the significance of the reversibility of these reactions will be assessed. The mechanism involved in the formation of the two bond types is to be studied along with the question of whether this is a regulated process. The active site of the enzyme is to be probed by use of chemical modifying agents. In addition, compounds that may serve as active-site directed inactivators will be synthesized and studied. The enzyme has been shown to have unique behavior with detergents. In the presence of SDS it is completely inactivated, and upon the addition of Triton X-100 it is completely reactivated. Studies are planned that will explore the molecular events associated with this behavior.

右旋蔗糖酶催化形成含有α 1-6和的葡聚糖

项目成果

Dextransucrase: donor substrate reactions.

葡聚糖蔗糖酶：供体底物反应。

• DOI: 10.1016/0003-9861(81)90151-x
• 发表时间: 1981
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Jung,SM;Mayer,RM
• 通讯作者: Mayer,RM

Recovery of octyl beta-glucoside from detergent/protein mixtures.

从洗涤剂/蛋白质混合物中回收辛基 β-葡萄糖苷。

• DOI: 10.1016/0003-2697(90)90385-m
• 发表时间: 1990
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: Kobs,SF

A D-glucosylated form of dextransucrase: preparation and characteristics.

D-葡萄糖基化形式的葡聚糖蔗糖酶：制备和特性。

• DOI: 10.1016/0008-6215(83)84022-1
• 发表时间: 1983
• 期刊: Carbohydrate research
• 影响因子: 3.1
• 作者: Parnaik,VK;Luzio,GA;Grahame,DA;Ditson,SL;Mayer,RM
• 通讯作者: Mayer,RM

Formation of alpha-(1-->6), alpha-(1-->3), and alpha-(1-->2) glycosidic linkages by dextransucrase from Streptococcus sanguis in acceptor-dependent reactions.

在受体依赖性反应中，血链球菌的葡聚糖蔗糖酶形成 α-(1-->6)、α-(1-->3) 和 α-(1-->2) 糖苷键。

• DOI: 10.1016/0008-6215(93)80034-c
• 发表时间: 1993
• 期刊: Carbohydrate research
• 影响因子: 3.1
• 作者: Bhattacharjee,MK;Mayer,RM
• 通讯作者: Mayer,RM

Affinity purification of dextransucrase from Streptococcus sanguis ATCC 10558.

血链球菌 ATCC 10558 中葡聚糖蔗糖酶的亲和纯化。

• DOI: 10.1016/0008-6215(90)80055-8
• 发表时间: 1990
• 期刊: Carbohydrate research
• 影响因子: 3.1
• 作者: Kobs,SF;Husman,DW;Cawthern,KM;Mayer,RM
• 通讯作者: Mayer,RM