MECHANISMS OF HEXOSE EPIMERASES AND OXIDOREDUCTASES

己糖差向异构酶和氧化还原酶的机制

• 批准号: 3285277
• 负责人: ROBERT MAYER
• 金额: $ 9.91万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-17 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3285277
• 关键词: Salmonella; Streptomyces; carbohydrate biosynthesis; carbohydrate metabolism; enzyme mechanism; enzyme structure; enzyme substrate complex; fucose; hydrogen transport; isomerase; mannose; oxidoreductase; stereochemistry; sugar nucleotide

A series of enzymological, stereochemical and mechanistic studies are proposed on reactions in hexose metabolism, which lead to the reductive replacement of hydroxyl groups by hydrogen and to the epimerization of chiral centers in the carbohydrate chain. These studies will deal with the GDP-mannose oxidoreductase reaction involved in fucose biosynthesis, with the CDP-4-keto-6-deoxyglucose 3-reductase system from Salmonella, with the formation of 2,6-dideoxyhexoses in several streptomyces, with the epimerization of GDP-mannose to GDP-L-galactose (and GDP-L-fucose) by plant enzymes, and with the epimerization of D mannuronic to L guluronic acid residues in the course of alginic acid formation.

进行了一系列的酶学、立体化学和机理研究

项目成果

Purification and characterization of TDP-D-glucose 4,6-dehydratase from anthracycline-producing streptomycetes.

产蒽环类链霉菌 TDP-D-葡萄糖 4,6-脱水酶的纯化和表征。

• DOI: 10.1099/00221287-138-4-779
• 发表时间: 1992
• 期刊: Journal of general microbiology
• 作者: Thompson,MW;Strohl,WR;Floss,HG
• 通讯作者: Floss,HG