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Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture

了解和利用生物固氮来改善巴西农业

基本信息

批准号：
BB/N013476/1
负责人：
Raymond Alan Dixon
金额：
$ 169.48万
依托单位：
John Innes Centre
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2016
资助国家：
英国
起止时间：
2016 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FN013476%2F1
关键词：
Understanding Exploiting Biological Nitrogen Fixation

项目摘要

After water, available nitrogen is the most limiting nutrient for plant growth. Although the demand for fixed nitrogen can be met by the use of chemical fertilisers, their use in agriculture has resulted in major environmental and economic impacts. At present less than half of the nitrogen used by farmers is assimilated by crops. Excess nitrogen leaks into the environment, leading to significant effects on soil and water quality, biodiversity, and atmospheric pollution. However, some bacteria can perform a process known as biological nitrogen fixation, which converts nitrogen gas from the atmosphere into ammonia, a source of nitrogen that can be assimilated by animals and plants. This provides a more sustainable alternative to the use of synthetic fertilisers.Although soil-dwelling nitrogen fixing bacteria can associate with food and energy crops, they do not readily release their fixed nitrogen for the benefit of the plant, although they can have beneficial effects on plant growth. In Brazil, significant increases in crop yield have been observed in response to inoculation with nitrogen fixing bacteria, some of which have an endophytic lifestyle and can gain access to intercellular spaces in plant roots and shoots. This provides an opportunity to engineer closer associations between nitrogen-fixing bacteria and food and energy crops, in which fixed nitrogen is delivered more effectively to the plant. This proposal for a Virtual Joint Centre (VJC) brings together a team of Brazilian and UK investigators focused towards understanding and exploiting plant-diazotroph interactions as a means to enhance agricultural productivity in Brazil. Using fundamental knowledge of nitrogen regulation in endophytic nitrogen fixing bacteria, we will engineer strains, which excrete ammonium to benefit crop growth, and examine the potential of these modified strains as inoculants. We will also design selection strategies to isolate associative diazotrophs that are competitive in the rhizosphere in relation to plant varieties that respond well and have a growth advantage in the presence of these endophytes. We will identify genes required for efficient plant-microbe interactions and quantitate the level of biological, nitrogen fixation in relation to crop yield. Finally, we will we examine the potential for the use of native and engineered strains as inoculants. To achieve these objectives, we will exploit strong synergies and world-leading expertise of UK and Brazilian researchers in biological nitrogen fixation, microbial population dynamics, bacterial and plant genetics, genomics and synthetic biology, in order to engineer efficient cultivar-endophyte combinations and develop improved inoculant technologies. Enhancing nitrogen fixation in our target crops promises substantial benefits for Brazilian agriculture, while decreasing the use and environmental impact of industrial nitrogen fertilisers.

除水外，有效氮是植物生长的最大限制性养分。虽然对固定氮的需求可以通过使用化学肥料来满足，但它们在农业中的使用导致了重大的环境和经济影响。目前，农民使用的氮中只有不到一半被作物吸收。过量的氮泄漏到环境中，对土壤和水质、生物多样性和大气污染产生重大影响。然而，一些细菌可以执行一个被称为生物固氮的过程，将大气中的氮气转化为氨，氨是一种可以被动物和植物吸收的氮源。这为使用合成肥料提供了一个更可持续的替代方案。虽然土壤固氮细菌可以与粮食和能源作物结合，但它们不容易释放其固定的氮对植物有益，尽管它们可以对植物生长产生有益影响。在巴西，接种固氮细菌后，作物产量显著增加，其中一些细菌具有内生生活方式，可以进入植物根和芽的细胞间隙。这为设计固氮细菌与粮食和能源作物之间更紧密的联系提供了机会，其中固定氮更有效地输送到植物中。这个虚拟联合中心（VJC）的提议汇集了一个巴西和英国的研究团队，专注于了解和利用植物-固氮生物的相互作用，作为提高巴西农业生产力的一种手段。利用内生固氮细菌氮调节的基础知识，我们将工程菌株，分泌铵，有利于作物生长，并检查这些修饰菌株作为接种剂的潜力。我们还将设计选择策略，以隔离相关的固氮菌，在根际竞争力的植物品种，反应良好，并在这些内生菌的存在下具有生长优势。我们将确定有效的植物-微生物相互作用所需的基因，并定量生物固氮水平与作物产量的关系。最后，我们将研究使用天然和工程菌株作为接种剂的潜力。为了实现这些目标，我们将利用英国和巴西研究人员在生物固氮，微生物种群动态，细菌和植物遗传学，基因组学和合成生物学方面的强大协同作用和世界领先的专业知识，以设计有效的栽培-内生菌组合并开发改进的接种剂技术。增强我们目标作物的固氮作用将为巴西农业带来巨大利益，同时减少工业氮肥的使用和对环境的影响。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Interactions between paralogous bacterial enhancer-binding proteins enable metal-dependent regulation of alternative nitrogenases in Azotobacter vinelandii.

DOI：
10.1111/mmi.14955
发表时间：
2022-07
期刊：
MOLECULAR MICROBIOLOGY
影响因子：
3.6
作者：
Appia-Ayme, Corinne;Little, Richard;Chandra, Govind;Martins, Carlo de Oliveira;Batista, Marcelo Bueno;Dixon, Ray
通讯作者：
Dixon, Ray

PHB Biosynthesis Counteracts Redox Stress in Herbaspirillum seropedicae.

DOI：
10.3389/fmicb.2018.00472
发表时间：
2018
期刊：
Frontiers in microbiology
影响因子：
5.2
作者：
Batista MB;Teixeira CS;Sfeir MZT;Alves LPS;Valdameri G;Pedrosa FO;Sassaki GL;Steffens MBR;de Souza EM;Dixon R;Müller-Santos M
通讯作者：
Müller-Santos M

Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system.

DOI：
10.1073/pnas.2302732120
发表时间：
2023-07-25
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Boyer, Nathaniel R.;Tokmina-Lukaszewska, Monika;Batista, Marcelo Bueno;Mus, Florence;Dixon, Ray;Bothner, Brian;Peters, John W.
通讯作者：
Peters, John W.

Disrupting hierarchical control of nitrogen fixation enables carbon-dependent regulation of ammonia excretion in soil diazotrophs

破坏固氮的层次控制使得土壤固氮生物中氨排泄的碳依赖性调节成为可能