PLACENTAL TRANSFER OF FLUORIDE

氟化物的胎盘转移

• 批准号: 3221476
• 负责人: JAMES W BAWDEN
• 金额: $ 14.44万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3221476
• 关键词: biological models; dental caries inhibitor; dosage; drug adverse effect; drug metabolism; gestational age; guinea pigs; ingested fluoride therapy; mathematical model; placental transfer; plasma; preventive dentistry; tooth enamel

The clinical and biological effects of prenatal administration of fluorides in an effort to prevent dental caries in the offspring are incompletely defined. Although numerous studies have explored maternal-fetal fluoride metabolism and uptake of fluoride in fetal hard tissues, the data were of limited value because of difficulties in accurately assaying for the low plasma [F], especially in fetal plasma where levels are extremely low. Also, a practical, relevant and relatively inexpensive animal model system had not been established. We have refined the analytical method to provide accurate assay of F at the low levels involved. And, we have established the guinea pig as useful, relevant model system for controlled experiments. This project is designed to extend our studies of the parameters and mechanisms concerning the placental transfer of fluorides, using the guinea pig as the model system. The proposed work is a logical extension of our previous studies, especially the findings resulting from experiments conducted with the support of our currently funded project grant (grant period: 8/1/86-7/31/89). Certain of the basic parameters of maternal- fetal fluoride metabolism have been established. Our data provide the basis for a hypothesis concerning the mathematical relationship between maternal plasma [F], fetal plasma [F], and enamel plasma [F]. This hypothesis should be tested. The effects of prenatal fluoride exposure on F uptake in enamel postnatally need to be explored. And, the basic mechanisms in placental transfer of F and F clearance from fetal plasma can now be studied. The project includes three Specific Aims: (1) To test the hypothesis that [F] in fetal plasma and developing enamel bear definable mathematical relationships to maternal plasma [F] when variations in chronic F exposure occur during gestation. The relationship between variations in maternal dose and maternal plasma [F] also will be established. (2) To determine the effect of prenatal fluoride status on F uptake in developing enamel postnatally. (3) To explore the mechanisms involved in the placental transfer of F and clearance from the fetal plasma.

产前应用氟化物的临床和生物学效应 在努力预防龋齿的后代是不完全的 已定义。尽管许多研究探索了母体-胎儿氟化物 胎儿硬组织中氟的代谢和摄取，数据为 价值有限，因为难以准确地进行低值检测 血浆[F]，特别是在水平极低的胎儿血浆中。 另外，一个实用的，相关的，相对便宜的动物模型系统 还没有得到证实。我们改进了分析方法，以提供 在涉及的低水平下准确地测定氟。而且，我们已经建立了 将豚鼠作为有用的、相关的模型系统进行对照实验。 这个项目的目的是扩大我们对参数和 使用豚鼠的胎盘转移氟化物的机制 以猪为模型系统。拟议的工作是我们的 先前的研究，尤指由实验得出的结果 在我们目前资助的项目赠款(赠款)的支持下进行 期间：8/1/86-7/31/)。母体的某些基本参数- 胎儿的氟化物代谢已经建立起来。我们的数据提供了 关于数学关系的一个假设的基础 母体血浆[F]、胎儿血浆[F]和釉质血浆[F]。这 假设应该得到检验。胎儿期氟暴露对围产儿健康的影响 出生后牙釉质对氟的摄取需要进一步研究。而且，基本的 胎盘转移胎儿血浆中F和F清除的机制 现在要研究一下。 该项目包括三个具体目标： (1)检验胎儿血浆和发育中牙釉质中[F]的假设 与母体血浆之间存在可定义的数学关系[F] 慢性氟暴露的变化发生在怀孕期间。两国关系 母体剂量和母体血浆[F]的变化也将是 已经成立了。(2)探讨产前氟化物状况对F的影响 出生后牙釉质发育中的摄取。(三)探索机制。 参与胎盘F的转移和从胎儿体内的清除 血浆。